Sequence of MPG

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Sequence of MPG

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Sequence of MDH1-PGK-GFP_2.0 : 6963 BP; 1638 A; 1960 C; 1796 G; 1569 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat 60

1 actttctggg gtggacatcc aaaccgttcg atcgaattca ttgcggtaaa acgttccgta 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca 120

61 ccttttatgt attgactctt atctcttcaa gtctagttcc aatccttgtc tctctgtcgt 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga 180

121 cttatacccg gtttgtccta tagacaccat tcgtcaagga cggggccgag tcccggttct 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt 240

181 tgtctaccag gggtctacgc cagggcggga gtcgtcaaag atctcttggt agtctacaaa 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ccagggtgcc ccaaggacct gaaaatgacc ctgtgcctta tttgaactaa ccaatcagtt 300

241 ggtcccacgg ggttcctgga cttttactgg gacacggaat aaacttgatt ggttagtcaa 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc 360

301 gcgaagagcg aagacaagcg cgcgaagacg aggggctcga gttattttct cgggtgttgg 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 cctcactcgg cgcgccagtc ctccgataga ctgcgtcgcc cgggtacccg tattcccaat 420

361 ggagtgagcc gcgcggtcag gaggctatct gacgcagcgg gcccatgggc ataagggtta 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 aaagcctctt gctgtttgca tccgaatcgt ggactcgctg atccttggga gggtctcctc 480

421 tttcggagaa cgacaaacgt aggcttagca cctgagcgac taggaaccct cccagaggag 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 agattgattg actgcccacc tcgggggtct ttcatttgga ggttccaccg agatttggag 540

481 tctaactaac tgacgggtgg agcccccaga aagtaaacct ccaaggtggc tctaaacctc 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 acccctgcct agggaccacc gacccccccg ccgggaggta agctggccag cggtcgtttc 600

541 tggggacgga tccctggtgg ctgggggggc ggccctccat tcgaccggtc gccagcaaag 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gtgtctgtct ctgtctttgt gcgtgtttgt gccggcatct aatgtttgcg cctgcgtctg 660

601 cacagacaga gacagaaaca cgcacaaaca cggccgtaga ttacaaacgc ggacgcagac 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tactagttag ctaactagct ctgtatctgg cggacccgtg gtggaactga cgagttctga 720

661 atgatcaatc gattgatcga gacatagacc gcctgggcac caccttgact gctcaagact 720

.......... .......... psi_pl.. .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 acacccggcc gcaaccctgg gagacgtccc agggactttg ggggccgttt ttgtggcccg 780

721 tgtgggccgg cgttgggacc ctctgcaggg tccctgaaac ccccggcaaa aacaccgggc 780

.......... .......... psi_pl.. .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 acctgaggaa gggagtcgat gtggaatccg accccgtcag gatatgtggt tctggtagga 840

781 tggactcctt ccctcagcta caccttaggc tggggcagtc ctatacacca agaccatcct 840

.......... .......... psi_pl.. .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gacgagaacc taaaacagtt cccgcctccg tctgaatttt tgctttcggt ttggaaccga 900

841 ctgctcttgg attttgtcaa gggcggaggc agacttaaaa acgaaagcca aaccttggct 900

.......... .......... psi_pl.. .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 agccgcgcgt cttgtctgct gcagcgctgc agcatcgttc tgtgttgtct ctgtctgact 960

901 tcggcgcgca gaacagacga cgtcgcgacg tcgtagcaag acacaacaga gacagactga 960

.......... .......... psi_pl.. .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gtgtttctgt atttgtctga aaattagggc cagactgtta ccactccctt aa gtttgacc 1020

961 cacaaagaca taaacagact tttaatcccg gtctgacaat ggtgagggaa ttcaaactgg 1020

.......... .......... psi_pl.. .......... gag ..........

.......... .......... .......... .......... .......... ..........

1021 ttaggtcact ggaaagatgt cgagcggatc gctcacaacc agtcggtaga tgtcaagaag 1080

1021 aatccagtga cctttctaca gctcgcctag cgagtgttgg tcagccatct acagttcttc 1080

.......... .......... psi_pl gag .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 agacgttggg ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg atggccgcga 1140

1081 tctgcaaccc aatggaagac gagacgtctt accggttgga aattgcagcc taccggcgct 1140

.......... .......... psi_pl gag .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gacggcacct ttaaccgaga cctcatcacc caggttaaga tcaaggtctt ttcacctggc 1200

1141 ctgccgtgga aattggctct ggagtagtgg gtccaattct agttccagaa aagtggaccg 1200

.......... .......... psi_pl gag .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ccgcatggac acccagacca ggtcccctac atcgtgacct gggaagcctt ggcttttgac 1260

1201 ggcgtacctg tgggtctggt ccaggggatg tagcactgga cccttcggaa ccgaaaactg 1260

.......... .......... psi_pl gag .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ccccctccct gggtcaagcc ctttgtacac cctaagcctc cgcctcctct tcctccatcc 1320

1261 gggggaggga cccagttcgg gaaacatgtg ggattcggag gcggaggaga aggaggtagg 1320

.......... .......... psi_pl gag .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gccccgtctc tcccccttga acctcctcgt tcgaccccgc ctcgatcctc cctttatcca 1380

1321 cggggcagag agggggaact tggaggagca agctggggcg gagctaggag ggaaataggt 1380

......... psi_pl gag MSCV_pri pLXSN_5_pri ........ pBABE_5_pri

.......... .......... ......... BglII(1411) ......... ..........

1381 gccctcactc cttctctagg cgccggaatt agatcttata tggggcaccc ccgccccttg 1440

1381 cgggagtgag gaagagatcc gcggccttaa tctagaatat accccgtggg ggcggggaac 1440

.......... psi_pl.. gag .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 taaacttccc tgaccctgac atgacaagag ttactaacag cccctctctc caagctcact 1500

1441 atttgaaggg actgggactg tactgttctc aatgattgtc ggggagagag gttcgagtga 1500

.......... gag .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tacaggctct ctacttagtc cagcacgaag tctggagacc tctggcggca gcctaccaag 1560

1501 atgtccgaga gatgaatcag gtcgtgcttc agacctctgg agaccgccgt cggatggttc 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 aacaactgga ccgaccggtg gtacctcacc cttaccgagt cggcgacaca gtgtgggtcc 1620

1561 ttgttgacct ggctggccac catggagtgg gaatggctca gccgctgtgt cacacccagg 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gccgacacca gactaagaac ctagaacctc gctggaaagg accttacaca gtcctgctga 1680

1621 cggctgtggt ctgattcttg gatcttggag cgacctttcc tggaatgtgt caggacgact 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... PmlI(1736)

1681 ccacccccac cgccctcaaa gtagacggca tcgcagcttg gatacacgcc gcccacgtga 1740

1681 ggtgggggtg gcgggagttt catctgccgt agcgtcgaac ctatgtgcgg cgggtgcact 1740

.......... .......... .......... .......... pBMN_5_pri ..........

.......... .......... .......... .......... .......... ..........

1741 aggctgccga ccccgggggt ggaccatcct ctagactgcc ggatcaattc ctaccgggta 1800

1741 tccgacggct ggggccccca cctggtagga gatctgacgg cctagttaag gatggcccat 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcggcccc gctgggcact 1860

1801 cccctccgcg aaaagggttc cgtcagacct cgtacgcgaa atcgccgggg cgacccgtga 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa 1920

1861 accgcgatgt gttcaccgga gaccggagcg tgtgtaaggt gtaggtggcc atccgcggtt 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag 1980

1921 ggccgaggca agaaaccacc ggggaagcgc ggtggaagat gaggagggga tcagtccttc 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc 2040

1981 aagggggggc ggggcgtcga gcgcagcacg tcctgcactg tttaccttca tcgtgcagag 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... StuI(2090) ..........

2041 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc 2100

2041 tgatcagagc acgtctacct gtcgtggcga ctcgttacct tcgcccatcc ggaaaccccg 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 agcggccaat agcagctttg gctccttcgc tttctgggct cagaggctgg gaaggggtgg 2160

2101 tcgccggtta tcgtcgaaac cgaggaagcg aaagacccga gtctccgacc cttccccacc 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg 2220

2161 caggcccccg cccgagtccc cgcccgagtc cccgccccgc ccgcgggctt ccaggaggcc 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc 2280

2221 tccgggccgt aagacgtgcg aagttttcgc gtgcagacgg cgcgacaaga ggagaaggag 2280

.......... mPGK_F_pri .......... .......... .......... ..........

.......... .......... NcoI(2304) .......... .......... ..........

2281 atctccgggc ctttcgacct gcaccatggt gagcaagggc gaggagctgt tcaccggggt 2340

2281 tagaggcccg gaaagctgga cgtggtacca ctcgttcccg ctcctcgaca agtggcccca 2340

.......... .......... .......... .......... EGFP ORF2 ..........

.......... .......... .......... .......... .......... ..........

2341 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg 2400

2341 ccacgggtag gaccagctcg acctgccgct gcatttgccg gtgttcaagt cgcacaggcc 2400

.......... ..... EGFP ORF2 EGFP_N_pri ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg 2460

2401 gctcccgctc ccgctacggt ggatgccgtt cgactgggac ttcaagtaga cgtggtggcc 2460

.......... .......... EGFP ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 caagctgccc gtgccctggc ccaccctcgt gaccaccttc acctacggcg tgcagtgctt 2520

2461 gttcgacggg cacgggaccg ggtgggagca ctggtggaag tggatgccgc acgtcacgaa 2520

.......... .......... EGFP ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg 2580

2521 gtcggcgatg gggctggtgt acttcgtcgt gctgaagaag ttcaggcggt acgggcttcc 2580

.......... .......... EGFP ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga 2640

2581 gatgcaggtc ctcgcgtggt agaagaagtt cctgctgccg ttgatgttct gggcgcggct 2640

.......... .......... EGFP ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa 2700

2641 ccacttcaag ctcccgctgt gggaccactt ggcgtagctc gacttcccgt agctgaagtt 2700

.......... .......... EGFP ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta 2760

2701 cctcctgccg ttgtaggacc ccgtgttcga cctcatgttg atgttgtcgg tgttgcagat 2760

.......... .......... EGFP ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat 2820

2761 atagtaccgg ctgttcgtct tcttgccgta gttccacttg aagttctagg cggtgttgta 2820

.......... .......... EGFP ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg 2880

2821 gctcctgccg tcgcacgtcg agcggctggt gatggtcgtc ttgtgggggt agccgctgcc 2880

.......... .......... EGFP ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc 2940

2881 ggggcacgac gacgggctgt tggtgatgga ctcgtgggtc aggcgggact cgtttctggg 2940

.......... .......... EGFP ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct 3000

2941 gttgctcttc gcgctagtgt accaggacga cctcaagcac tggcggcggc cctagtgaga 3000

.......... ..... EGFP ORF2 EGFP_C_pri ..... .......... ..........

.......... .......... .......... .......... NotI(3044) SalI(3051)

3001 cggcatggac gagctgtaca agtaatgaat taattaagaa ttgcggccgc gtcgacctgc 3060

3001 gccgtacctg ctcgacatgt tcattactta attaattctt aacgccggcg cagctggacg 3060

.......... EGFP ORF2 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 agccaagctt atcgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga 3120

3061 tcggttcgaa tagctatttt attttctaaa ataaatcaga ggtctttttc cccccttact 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... XhoI(3163) ..........

3121 aagaccccac ctgtaggttt ggcaagaatt cgtttaaacg ggctcgagtg gtctcataca 3180

3121 ttctggggtg gacatccaaa ccgttcttaa gcaaatttgc ccgagctcac cagagtatgt 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gaacttataa gattcccaaa tccaaagaca tttcacgttt atggtgattt cccagaacac 3240

3181 cttgaatatt ctaagggttt aggtttctgt aaagtgcaaa taccactaaa gggtcttgtg 3240

.......... .......... .......... .......... .......... H1_pri

.......... ......... BamHI(3261) ......... .......... ..........

3241 atagcgacat gcaaatattg ggatccgcta gcttaagtaa cgccattttg caaggcatgg 3300

3241 tatcgctgta cgtttataac cctaggcgat cgaattcatt gcggtaaaac gttccgtacc 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 aaaatacata actgagaata gagaagttca gatcaaggtt aggaacagag agacagcaga 3360

3301 ttttatgtat tgactcttat ctcttcaagt ctagttccaa tccttgtctc tctgtcgtct 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac 3420

3361 tatacccggt ttgtcctata gacaccattc gtcaaggacg gggccgagtc ccggttcttg 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 agatggtccc cagatgcggt cccgccctca gcagtttcta gagaaccatc agatgtttcc 3480

3421 tctaccaggg gtctacgcca gggcgggagt cgtcaaagat ctcttggtag tctacaaagg 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 agggtgcccc aaggacctga aaatgaccct gtgccttatt tgaactaacc aatcagttcg 3540

3481 tcccacgggg ttcctggact tttactggga cacggaataa acttgattgg ttagtcaagc 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc 3600

3541 gaagagcgaa gacaagcgcg cgaagacgag gggctcgagt tattttctcg ggtgttgggg 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tcactcggcg cgccagtcct ccgatagact gcgtcgcccg ggtacccgtg tatccaataa 3660

3601 agtgagccgc gcggtcagga ggctatctga cgcagcgggc ccatgggcac ataggttatt 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga 3720

3661 tgggagaacg tcaacgtagg ctgaacacca gagcgacaag gaaccctccc agaggagact 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gtgattgact acccgtcagc gggggtcttt catgggtaac agtttcttga agttggagaa 3780

3721 cactaactga tgggcagtcg cccccagaaa gtacccattg tcaaagaact tcaacctctt 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 caacattctg agggtaggag tcgaatatta agtaatcctg actcaattag ccactgtttt 3840

3781 gttgtaagac tcccatcctc agcttataat tcattaggac tgagttaatc ggtgacaaaa 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gaatccacat actccaatac tcctgaaata gttcattatg gacagcgcag aagagctggg 3900

3841 cttaggtgta tgaggttatg aggactttat caagtaatac ctgtcgcgtc ttctcgaccc 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gagaattaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 3960

3901 ctcttaatta agcattagta ccagtatcga caaaggacac actttaacaa taggcgagtg 3960

.......... .......... M13_r_pri ........ M13_pUC_r_pri ........

.......... .......... .......... .......... .......... ..........

3961 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 4020

3961 ttaaggtgtg ttgtatgctc ggccttcgta tttcacattt cggaccccac ggattactca 4020

.......... .......... lac_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 4080

4021 ctcgattgag tgtaattaac gcaacgcgag tgacgggcga aaggtcagcc ctttggacag 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 4140

4081 cacggtcgac gtaattactt agccggttgc gcgcccctct ccgccaaacg cataacccgc 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 4200

4141 gagaaggcga aggagcgagt gactgagcga cgcgagccag caagccgacg ccgctcgcca 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 4260

4201 tagtcgagtg agtttccgcc attatgccaa taggtgtctt agtcccctat tgcgtccttt 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 4320

4261 cttgtacact cgttttccgg tcgttttccg gtccttggca tttttccggc gcaacgaccg 4320

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

4321 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 4380

4321 caaaaaggta tccgaggcgg ggggactgct cgtagtgttt ttagctgcga gttcagtctc 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 4440

4381 caccgctttg ggctgtcctg atatttctat ggtccgcaaa gggggacctt cgagggagca 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 4500

4441 cgcgagagga caaggctggg acggcgaatg gcctatggac aggcggaaag agggaagccc 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 4560

4501 ttcgcaccgc gaaagagtat cgagtgcgac atccatagag tcaagccaca tccagcaagc 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 4620

4561 gaggttcgac ccgacacacg tgcttggggg gcaagtcggg ctggcgacgc ggaataggcc 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 4680

4621 attgatagca gaactcaggt tgggccattc tgtgctgaat agcggtgacc gtcgtcggtg 4680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 4740

4681 accattgtcc taatcgtctc gctccataca tccgccacga tgtctcaaga acttcaccac 4740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 4800

4741 cggattgatg ccgatgtgat cttcctgtca taaaccatag acgcgagacg acttcggtca 4800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4801 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 4860

4801 atggaagcct ttttctcaac catcgagaac taggccgttt gtttggtggc gaccatcgcc 4860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 4920

4861 accaaaaaaa caaacgttcg tcgtctaatg cgcgtctttt tttcctagag ttcttctagg 4920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 4980

4921 aaactagaaa agatgcccca gactgcgagt caccttgctt ttgagtgcaa ttccctaaaa 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 5040

4981 ccagtactct aatagttttt cctagaagtg gatctaggaa aatttaattt ttacttcaaa 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 5100

5041 atttagttag atttcatata tactcatttg aaccagactg tcaatggtta cgaattagtc 5100

.......... .......... .......... .......... .......... Ampicillin

.......... .......... .......... .......... .......... ..........

5101 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 5160

5101 actccgtgga tagagtcgct agacagataa agcaagtagg tatcaacgga ctgaggggca 5160

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 5220

5161 gcacatctat tgatgctatg ccctcccgaa tggtagaccg gggtcacgac gttactatgg 5220

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 5280

5221 cgctctgggt gcgagtggcc gaggtctaaa tagtcgttat ttggtcggtc ggccttcccg 5280

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 5340

5281 gctcgcgtct tcaccaggac gttgaaatag gcggaggtag gtcagataat taacaacggc 5340

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 5400

5341 ccttcgatct cattcatcaa gcggtcaatt atcaaacgcg ttgcaacaac ggtaacgatg 5400

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 5460

5401 tccgtagcac cacagtgcga gcagcaaacc ataccgaagt aagtcgaggc caagggttgc 5460

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 5520

5461 tagttccgct caatgtacta gggggtacaa cacgtttttt cgccaatcga ggaagccagg 5520

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 5580

5521 aggctagcaa cagtcttcat tcaaccggcg tcacaatagt gagtaccaat accgtcgtga 5580

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 5640

5581 cgtattaaga gaatgacagt acggtaggca ttctacgaaa agacactgac cactcatgag 5640

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 5700

5641 ttggttcagt aagactctta tcacatacgc cgctggctca acgagaacgg gccgcagtta 5700

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 5760

5701 tgccctatta tggcgcggtg tatcgtcttg aaattttcac gagtagtaac cttttgcaag 5760

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 5820

5761 aagccccgct tttgagagtt cctagaatgg cgacaactct aggtcaagct acattgggtg 5820

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 5880

5821 agcacgtggg ttgactagaa gtcgtagaaa atgaaagtgg tcgcaaagac ccactcgttt 5880

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 5940

5881 ttgtccttcc gttttacggc gttttttccc ttattcccgc tgtgccttta caacttatga 5940

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 6000

5941 gtatgagaag gaaaaagtta taataacttc gtaaatagtc ccaataacag agtactcgcc 6000

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

6001 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 6060

6001 tatgtataaa cttacataaa tctttttatt tgtttatccc caaggcgcgt gtaaaggggc 6060

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 6120

6061 ttttcacggt ggactgcaga ttctttggta ataatagtac tgtaattgga tatttttatc 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 6180

6121 cgcatagtgc tccgggaaag cagagcgcgc aaagccacta ctgccacttt tggagactgt 6180

.......... .......... .......... .......... .......... pGEX_3_pri

.......... .......... .......... .......... .......... ..........

6181 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 6240

6181 gtacgtcgag ggcctctgcc agtgtcgaac agacattcgc ctacggccct cgtctgttcg 6240

pGEX_3_pri .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 6300

6241 ggcagtcccg cgcagtcgcc cacaaccgcc cacagccccg accgaattga tacgccgtag 6300

.......... .......... .......... .......... .......... ..........

.......... .......... NdeI(6327) .......... .......... ..........

6301 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 6360

6301 tctcgtctaa catgactctc acgtggtata cgccacactt tatggcgtgt ctacgcattc 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 6420

6361 ctcttttatg gcgtagtccg cggtaagcgg taagtccgac gcgttgacaa cccttcccgc 6420

.......... .......... .......... .......... lacZ_a ..........

.......... .......... .......... .......... .......... ..........

6421 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 6480

6421 tagccacgcc cggagaagcg ataatgcggt cgaccgcttt ccccctacac gacgttccgc 6480

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc 6540

6481 taattcaacc cattgcggtc ccaaaagggt cagtgctgca acattttgct gccggtcacg 6540

.......... .......... lacZ_a ..... M13_pUC_f M1_f20_pri .....

.......... .......... .......... .......... .......... ..........

6541 caagctcgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg 6600

6541 gttcgagcga gagggaatac gctgaggacg taatccttcg tcgggtcatc atccaactcc 6600

.......... .......... .......... tet .......... ..........

.......... .......... .......... .......... .......... ..........

6601 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc 6660

6601 ggcaactcgt ggcggcggcg ttccttacca cgtacgttcc tctaccgcgg gttgtcaggg 6660

.......... .......... tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg 6720

6661 ggccggtgcc ccggacggtg gtatgggtgc ggctttgttc gcgagtactc gggcttcacc 6720

.......... .......... tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg 6780

6721 gctcgggcta gaaggggtag ccactacagc cgctatatcc gcggtcgttg gcgtggacac 6780

.......... ...... tet pBRrevBam_pri ...... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgattagtc caatttgtta 6840

6781 cgcggccact acggccggtg ctacgcaggc cgcatctcct agctaatcag gttaaacaat 6840

.......... tet .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 aagacaggat atcagtggtc caggctctag ttttgactca acaatatcac cagctgaagc 6900

6841 ttctgtccta tagtcaccag gtccgagatc aaaactgagt tgttatagtg gtcgacttcg 6900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 ctatagagta cgagccatag ataaaataaa agattttatt tagtctccag aaaaaggggg 6960

6901 gatatctcat gctcggtatc tattttattt tctaaaataa atcagaggtc tttttccccc 6960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 gaa....... .......... .......... .......... .......... .......... 6963

6961 ctt....... .......... .......... .......... .......... .......... 6963

.......... .......... .......... .......... .......... ..........