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Mammalian Plasmids


 pEXP1-DEST_(Expressway_in_vitro) to pEYFP-N1 | pFB-Luc_(ViraPort) to pGene_V5-His_C_(GeneSwitch) | pGeneClip_Basic to pGeneClip_Puromycin | pGlow_TOPO to phrG-B | phRG-TK to phRL-TK | phRL-TK(Int-) to pIRES2-EGFP | pIRESbleo3 to pIRESpuro3 | 

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Plasmids 0 - 4Descriptions
small image of pEXP1-DEST_(Expressway_in_vitro)pEXP1-DEST (Expressway in vitro) - Vendor:Invitrogen; Vector Type: In vitro; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, Xpress (Nterm); Bacteria Resistance: N/a; Mammalian Selection: Zeocin; Catalog Number: V96001
small image of pEXP2-DEST_(Expressway_in_vitro)pEXP2-DEST (Expressway in vitro) - Vendor:Invitrogen; Vector Type: In vitro; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, Xpress (Cterm); Bacteria Resistance: N/a; Mammalian Selection: Zeocin; Catalog Number: V96002
small image of pEYFP-1pEYFP-1 - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: No promoter; Promoter: none; Sequencing Primer: EGFP-N; Sequencing Primer Sequence: 5'd[CGTCGCCGTCCAGCTCGACCAG]3'; Bacteria Resistance: Kanamycin; Mammalian Selection: Neomycin; Catalog Number: 6007-1; Comments:Yellow variant of GFP reporter. Used to monitor transcriptio...
small image of pEYFP-C1pEYFP-C1 - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: Constitutive; Promoter: CMV; Sequencing Primer: EGFP-C; Sequencing Primer Sequence: 5'd[CATGGTCCTGCTGGAGTTCGTG]; Tag: EYFP (Nterm); Bacteria Resistance: Kanamycin; Mammalian Selection: Neomycin; Catalog Number: 6006-1; Comments:Yellow variant of GFP tag
small image of pEYFP-N1pEYFP-N1 - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: Constitutive; Promoter: CMV; Sequencing Primer: EGFP-N; Sequencing Primer Sequence: 5'd[CGTCGCCGTCCAGCTCGACCAG]3'; Tag: EYFP (Cterm); Bacteria Resistance: Kanamycin; Mammalian Selection: Neomycin; Catalog Number: 6006-1; Comments:Yellow variant of GFP tag

Plasmids 5 - 9Descriptions
small image of pFB-Luc_(ViraPort)pFB-Luc (ViraPort) - Vendor:Stratagene; Vector Type: Mammalian; Viral/Non-viral: Viral; Stable/Transient: Stable (retroviral); Constitutive/Inducible: Constitutive; Bacteria Resistance: Ampicillin; Catalog Number: 240030; Comments:Retroviral vector containing luciferase, useful for optimizing transfection efficiency.
small image of pFB-Neo_(ViraPort)pFB-Neo (ViraPort) - Vendor:Stratagene; Vector Type: Mammalian; Viral/Non-viral: Viral; Stable/Transient: Stable (retroviral); Constitutive/Inducible: Constitutive; Bacteria Resistance: Ampicillin; Mammalian Selection: neo; Catalog Number: 217561; Comments:Retroviral expression vector that is selectable; must co-transfect with gag-pol & env protein
small image of pGene_V5-His_A_(GeneSwitch)pGene/V5-His A (GeneSwitch) - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Inducible; Promoter: Gal4-E1b; Expression Level: Tightly controlled (activate with mifepristone); Sequencing Primer: pGene Fwd; Sequencing Primer Sequence: 5'd[CTGCTATTCTGCTCAACCT]3'; Tag: 6X His, V5; Bacteria Resistance: Ampicillin; Mammal...
small image of pGene_V5-His_B_(GeneSwitch)pGene/V5-His B (GeneSwitch) - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Inducible; Promoter: Gal4-E1b; Expression Level: Tightly controlled (activate with mifepristone); Sequencing Primer: pGene Fwd; Sequencing Primer Sequence: 5'd[CTGCTATTCTGCTCAACCT]3'; Tag: 6X His, V5; Bacteria Resistance: Ampicillin; Mammal...
small image of pGene_V5-His_C_(GeneSwitch)pGene/V5-His C (GeneSwitch) - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Inducible; Promoter: Gal4-E1b; Expression Level: Tightly controlled (activate with mifepristone); Sequencing Primer: pGene Fwd; Sequencing Primer Sequence: 5'd[CTGCTATTCTGCTCAACCT]3'; Tag: 6X His, V5; Bacteria Resistance: Ampicillin; Mammal...

Plasmids 10 - 14Descriptions
small image of pGeneClip_BasicpGeneClip Basic - Vendor:Promega; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Expression Level: High; Sequencing Primer: T7 Fwd; Bacteria Resistance: Ampicillin; Catalog Number: C8750; GenBank Accession Number:AY744385
small image of pGeneClip_hMGFPpGeneClip hMGFP - Vendor:Promega; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Expression Level: High; Sequencing Primer: T7 Fwd; Tag: GFP; Bacteria Resistance: Ampicillin; Catalog Number: C8790; GenBank Accession Number:AY744386; Comments:Hairpin cloning system
small image of pGeneClip_HygromycinpGeneClip Hygromycin - Vendor:Promega; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Expression Level: High; Sequencing Primer: T7 Fwd; Bacteria Resistance: Ampicillin; Mammalian Selection: Hygromycin; Catalog Number: C8770; GenBank Accession Number:AY745745; Comments:Hairpin cloning system; unique drug resistance
small image of pGeneClip_NeomycinpGeneClip Neomycin - Vendor:Promega; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Expression Level: High; Sequencing Primer: T7 Fwd; Bacteria Resistance: Ampicillin; Mammalian Selection: G418; Catalog Number: C8780; GenBank Accession Number:AY745746; Comments:Hairpin cloning system; unique drug resistance
small image of pGeneClip_PuromycinpGeneClip Puromycin - Vendor:Promega; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Expression Level: High; Sequencing Primer: T7 Fwd; Bacteria Resistance: Ampicillin; Mammalian Selection: Puromycin; Catalog Number: C8760; GenBank Accession Number:AY745747; Comments:Hairpin cloning system; unique drug resistance

Plasmids 15 - 19Descriptions
small image of pGlow_TOPOpGlow TOPO - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Bacteria Resistance: Ampicillin; Mammalian Selection: G418; Catalog Number: K483001; Comments:Drives GFP expression
small image of pHcRed1-1pHcRed1-1 - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: No promoter; Promoter: none; Bacteria Resistance: Kanamycin; Mammalian Selection: Neomycin; Catalog Number: 632411; Comments:Far-red fluorescent reporter. Used to monitor transcription on cis-regulatory elements.
small image of pHcRed1-C1pHcRed1-C1 - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: Constitutive; Promoter: CMV; Tag: HcRed1 (Nterm); Bacteria Resistance: Kanamycin; Mammalian Selection: Neomycin; Catalog Number: 632415; Comments:HcRed1 tag, far-red fluorescent protein
small image of pHcRed1-N1_1pHcRed1-N1/1 - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: Constitutive; Promoter: CMV; Tag: HcRed1 (Cterm); Bacteria Resistance: Kanamycin; Mammalian Selection: Neomycin; Catalog Number: 632424; Comments:HcRed1 tag, far-red fluorescent protein
small image of phrG-BphrG-B - Vendor:Promega; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Expression Level: High; Sequencing Primer: n/a; Bacteria Resistance: Ampicillin; Catalog Number: E6281; GenBank Accession Number:AF362550; Comments:Renilla luciferase reporter

Plasmids 20 - 24Descriptions
small image of phRG-TKphRG-TK - Vendor:Promega; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Expression Level: High; Sequencing Primer: n/a; Bacteria Resistance: Ampicillin; Catalog Number: E6291; GenBank Accession Number:AF362551; Comments:Renilla luciferase reporter
small image of phRL-CMVphRL-CMV - Vendor:Promega; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: n/a; Bacteria Resistance: Ampicillin; Catalog Number: E6271; GenBank Accession Number:AF362549; Comments:Renilla luciferase reporter
small image of phRL-nullphRL-null - Vendor:Promega; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Expression Level: High; Sequencing Primer: n/a; Bacteria Resistance: Ampicillin; Catalog Number: E6231; GenBank Accession Number:AF362546; Comments:Renilla luciferase reporter
small image of phRL-SV40phRL-SV40 - Vendor:Promega; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: SV40; Expression Level: High; Sequencing Primer: n/a; Bacteria Resistance: Ampicillin; Catalog Number: E6261; GenBank Accession Number:AF362548; Comments:Renilla luciferase reporter
small image of phRL-TKphRL-TK - Vendor:Promega; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: HSV-TK; Expression Level: High; Sequencing Primer: n/a; Bacteria Resistance: Ampicillin; Catalog Number: E6241; GenBank Accession Number:AF362545; Comments:Renilla luciferase reporter

Plasmids 25 - 29Descriptions
small image of phRL-TK(Int-)phRL-TK(Int-) - Vendor:Promega; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: HSV-TK; Expression Level: High; Sequencing Primer: n/a; Bacteria Resistance: Ampicillin; Catalog Number: E6251; GenBank Accession Number:AF362547; Comments:Renilla luciferase reporter
small image of pIRESpIRES - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: Constitutive; Promoter: CMV; Tag: IRES; Bacteria Resistance: Ampicillin; Mammalian Selection: Neomycin; Catalog Number: 631605; Comments:Express 2 genes on one transcript with IRES
small image of pIRES2-DsRed2pIRES2-DsRed2 - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: Constitutive; Promoter: CMV; Tag: IRES-DsRed2; Bacteria Resistance: Kanamycin; Mammalian Selection: Neomycin; Catalog Number: 632420; Comments:Express a gene and DsRed2 on one transcript
small image of pIRES2-DsRed-ExpresspIRES2-DsRed-Express - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: Constitutive; Promoter: CMV; Tag: IRES-DsRed; Bacteria Resistance: Kanamycin; Mammalian Selection: Neomycin; Catalog Number: 632463; Comments:Express a gene and DsRed on one transcript
small image of pIRES2-EGFPpIRES2-EGFP - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: Constitutive; Promoter: CMV; Tag: IRES-EGFP; Bacteria Resistance: Kanamycin; Mammalian Selection: Neomycin; Catalog Number: 6029-1; Comments:Express a gene and EGFP on one transcript

Plasmids 30 - 34Descriptions
small image of pIRESbleo3pIRESbleo3 - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: Constitutive; Promoter: CMV; Tag: IRES-Bleo; Bacteria Resistance: Ampicillin; Mammalian Selection: Zeocin; Catalog Number: 631622; Comments:Stably select with bleomycin, phleomycin, or zeocin
small image of pIREShyg3pIREShyg3 - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: Constitutive; Promoter: CMV; Tag: IRES-hygromycin; Bacteria Resistance: Ampicillin; Mammalian Selection: Hygromycin; Catalog Number: 631620; Comments:Stably select with hygromycin
small image of pIRESpuro3pIRESpuro3 - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: Constitutive; Promoter: CMV; Tag: IRES-puromycin; Bacteria Resistance: Ampicillin; Mammalian Selection: Puromycin; Catalog Number: 631619; Comments:Stably select with puromycin



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