Mammalian expression Plasmids
Friend MLV Env YFP to pAcGFP1-C1 | pAcGFP1-N1 to pAmCyan1-C1 | pAmCyan1-N1 to pBABE-neo | pbgal-Basic to pBigT | pBIND to pCAGIG | pCAT3-Basic to pcDNA_DEST40 | pcDNA3.1(-) to pcDNA3.1(+)_myc-His_C | pcDNA3.1_CT-GFP_Fusion to pcDNA3.1_Hygro(-) | pcDNA3.1_Hygro(+) to pcDNA3.1_Zeo(+) | pcDNA3.1D_V5-His-TOPO to pcDNA4_His_C | pcDNA4_His-Max_A to pcDNA4_myc-His_B | pcDNA4_myc-His_C to pcDNA5_FRT_TO | pcDNA5_FRT_TO-E to pcDNA6.2_V5-GW_Directional_TOPO | pcDNA6_BioEase-DEST to pcDNA6_myc-His_A | pcDNA6_myc-His_B to pCMV_myc_cyto | pCMV_myc_ER to pCMV-Myc | pCMV-Script to pCX4pur | pCXneo to pDNR-CMV | pDRIVE-CAG to pDsRed-Express-1 | pDsRed-Express-C1 to pECFP-C1 | pECFP-N1 to pEF1_myc-His_A | pEF1_myc-His_B to pEF1_V5_His_C | pEF4_His_A to pEF4_myc-His_B | pEF4_myc-His_C to pEF5_FRT_V5_DEST_Gateway | pEF5_FRT_V5_D-TOPO to pEF6_His_C | pEF6_myc-His_A to pEF-GFP | pEGFP-1 to pEGFP-N3 | pIRESneo3 to pST1374 |
|
|
All plasmid maps listed here are created using BVTech Plasmid.
If you do not have BVTech Plasmid, Click here to download the latest version of
BVTech Plasmid
BVTech Plasmid is DNA sequence analysis and plasmid drawing software for Windows PCs.You can
use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data,
align sequences, and much more.
Free to try, $39.98 to buy
Payments will be processed by PayPal. PayPal is the safer, easier way to pay.
|
|
| Plasmids 0 - 4 | Descriptions |
 | Friend MLV Env YFP - Gene/insert name: Envelope ;
Alternative names: Env, FrMLVgp3 ;
Gene/insert aliases: env ;
Species of gene(s): MuLV ;
Fusion proteins or tags: EYFP ;
Vector backbone: pcDNA3;
Cloning site 5': ? ;
Site destroyed during cloning: Don't Know ;
Cloning site 3': ? ;
Site destroyed during cloning: Don't Know ;
5' Sequencing primer: T7 ;
3' S... |
 | JP1520 - Vendor: Harvard Institute of Proteomics; Vector Type: Mammalian expression; Viral/Non-viral: Retroviral; Bacteria Resistance: Ampicillin; Mammalian Selection: Puromycin; Comments: Made by J. Pearlberg at HIP. Includes loxP site for recombinational cloning. |
 | Lamp1-RFP - Gene/insert name: lysosome associated membrane protein 1 ;
Alternative names: Lamp-1 ;
Gene/insert aliases: Lamp1, LGP120 ;
Species of gene(s): R. norvegicus (rat) ;
Fusion proteins or tags: RFP ;
Terminal: C terminal on backbone ;
Vector backbone: Modified Clontech Plasmid;
Cloning site 5': EcoRI ;
Site destroyed during cloning: No ;
Cloning site 3'... |
 | Gene/insert name: p70 alpha1 S6 kinase -
Alternative names: p70 S6 kinase ;
Gene/insert aliases: Rps6kb1 ;
Species of gene(s): R. norvegicus (rat);
Fusion proteins or tags: HA ;
Terminal: N terminal on insert ;
Vector backbone: PMT2 (Search Vectorpedia) ;
Cloning site 5': EcoRI ;
Site destroyed during cloning: No ;
Cloning site 3': EcoRI ;
Site destroye... |
 | pAcGFP1-C1 - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: Constitutive; Promoter: CMV; Tag: GFP (Nterm); Bacteria Resistance: Kanamycin; Mammalian Selection: Neomycin; Catalog Number: 632470, 632426; Comments:GFP fusion with selection for mammalian expression |
| Plasmids 5 - 9 | Descriptions |
 | pAcGFP1-N1 - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: Constitutive; Promoter: CMV; Tag: GFP (Cterm); Bacteria Resistance: Kanamycin; Mammalian Selection: Neomycin; Catalog Number: 632469, 632426; Comments:GFP fusion with selection for mammalian expression |
 | pACT - Vendor:Promega; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Bacteria Resistance: Ampicillin; Mammalian Selection: G418; Catalog Number: E2440; GenBank Accession Number:AF264723; Comments:Use for CheckMate mammalian 2-hybrid system |
_thu.jpg) | pAd/CMV/V5 DEST Gateway (ViraPower) - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Viral; Stable/Transient: Transient; Promoter: CMV; Sequencing Primer: CMVPro Fwd; Sequencing Primer Sequence: 5'd[CGCAAATGGGCGGTAGGCGTG]3'; Tag: V5; Bacteria Resistance: Ampicillin; Catalog Number: V49320; Comments:Easy to clone into other vectors |
 | pAd-RFP - Gene/insert name: RFP; Species of gene(s): Other; Vector backbone: pAdTrace-TO4 (Search Vector Database); 5' Sequencing primer: na (List of Sequencing Primers); Bacteria resistance: Kanamycin; High or low copy: High Copy; Grow in standard E. coli @ 37C: Yes; Selectable markers: Neomycin; If you did not originally clone this gene, from whom and where did you receive the plasmid used ... |
 | pAmCyan1-C1 - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: Constitutive; Promoter: CMV; Tag: AmCyan (Nterm); Bacteria Resistance: Kanamycin; Mammalian Selection: Neomycin; Catalog Number: 632441, 630050; Comments:AmCyan fluorescent protein fusion for mammalian expression |
| Plasmids 10 - 14 | Descriptions |
 | pAmCyan1-N1 - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: Constitutive; Promoter: CMV; Tag: AmCyan (Cterm); Bacteria Resistance: Kanamycin; Mammalian Selection: Neomycin; Catalog Number: 632442, 630050; Comments:AmCyan fluorescent protein fusion for mammalian expression |
 | pAsRed2-C1 - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: Constitutive; Promoter: CMV; Tag: AsRed2 (Nterm); Bacteria Resistance: Kanamycin; Mammalian Selection: Neomycin; Catalog Number: 632450, 630050; Comments:AsRed2 fluorescent protein fusion for mammalian expression |
 | pAsRed2-N1 - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: Constitutive; Promoter: CMV; Tag: AsRed2 (Cterm); Bacteria Resistance: Kanamycin; Mammalian Selection: Neomycin; Catalog Number: 632449, 630050; Comments:AsRed2 fluorescent protein fusion for mammalian expression |
 | pBABE-MN-IRES-E-GFP - Vendor:Nolan lab; Vector Type: Mammalian; Viral/Non-viral: Retroviral; Sequencing Primer: pBMN5'; Bacteria Resistance: Ampicillin |
 | Vector backbone: pBABE-neo -
5' Sequencing primer: pBABE 5' ;
3' Sequencing primer: pBABE 3' ;
Bacteria resistance: Ampicillin ;
High or low copy: High Copy ;
Grow in standard E. coli @ 37C: Yes ;
Selectable markers: Neomycin ;
Plasmid Provided In: DH5a ;
Principal Investigator: Bob Weinberg ;
Comments: Morgenstern JP, Land H., 1990, Nucleic Acids Research 18(12):3587-96. |
| Plasmids 15 - 19 | Descriptions |
 | pbgal-Basic - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: No promoter; Promoter: none; Bacteria Resistance: Ampicillin; Catalog Number: 631707; Comments:MCS upstream of b-gal reporter. Used to test cis-regulatory elements. |
 | pBI - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Tet responsive; Promoter: CMV; Bacteria Resistance: Ampicillin; Catalog Number: 6152-1; Comments:Allows 2 proteins to expressed off of one vector. Used in Tet-responsive system |
 | pBI-EGFP - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Tet responsive; Promoter: CMV; Bacteria Resistance: Ampicillin; Catalog Number: 6154-1; Comments:Express your gene and GFP from same vector. Tet-responsive. |
 | pBI-G - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Tet responsive; Promoter: CMV; Bacteria Resistance: Ampicillin; Catalog Number: 6150-1; Comments:Express your gene and b-galactosidase from same vector. Tet-responsive. |
 | pBigT - Vector Type: For making transgenic mice; Bacteria Resistance: Ampicillin; Mammalian Selection: Neomycin; Comments:Described in Srinivas et al. BMC Dev Biol (2001) vol 1, p.4. For use with pRosa26PA. See article for more information. |
| Plasmids 20 - 24 | Descriptions |
 | pBIND - Vendor:Promega; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Bacteria Resistance: Ampicillin; Catalog Number: E2440; Comments:Use for CheckMate mammalian 2-hybrid system |
 | pBlue TOPO - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His; Bacteria Resistance: Ampicillin; Mammalian Selection: Neomycin; Catalog Number: K483101; Comments:Drives lacZ expression |
 | pBudCE4.1 - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Constitutive/Inducible: Constitutive; Promoter: CMV or EF-1a; Sequencing Primer: CMVPro Fwd, EF1aPro Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3', 5'd[TCAAGCCTCAGACAGTGGTTC]3'; Bacteria Resistance: N/a; Mammalian Selection: Zeocin; Catalog Number: V53220; Comments:Dual expression in one plasmid |
 | pCAG-Cre_GFP - Gene/insert name: Cre-GFP fusion protein; Alternative names: Cre recombinase; Species of gene(s): Bacteriophage P1; Fusion proteins or tags: GFP; Terminal: C terminal on insert; Vector backbone: pCAGEN (Search Vector Database); Cloning site 5': EcoRI; Site destroyed during cloning: No; Cloning site 3': NotI; Site destroyed during cloning: No; 5' Sequencing primer: p... |
 | pCAGIG - Gene/insert name: IRES-EGFP ;
Alternative names: internal ribosomal entry site from Encephalomyocarditis virus
green fluorescent protein from Aequorea victoria ;
Vector backbone: pCAGEN;
Cloning site 5': NotI ;
Site destroyed during cloning: No ;
Cloning site 3': MscI ;
Site destroyed during cloning: Yes ;
5' Sequencing primer: pCAG-F ;
Bacteria resistance: ... |
| Plasmids 25 - 29 | Descriptions |
 | pCAT3-Basic - Vendor:Promega; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: no promoter; Expression Level: High; Sequencing Primer: T7 Fwd; Bacteria Resistance: Ampicillin; Catalog Number: E1871; GenBank Accession Number:U57024; Comments:CAT reporter; no eukaryotic promoter and enhancer sequences |
 | pCAT3-Control - Vendor:Promega; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: SV40; Expression Level: High; Sequencing Primer: T7 Fwd; Bacteria Resistance: Ampicillin; Catalog Number: E1851; GenBank Accession Number:U57025; Comments:CAT reporter; SV40 promoter and enhancer |
 | pCAT3-Enhancer - Vendor:Promega; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: no promoter; Expression Level: High; Sequencing Primer: T7 Fwd; Bacteria Resistance: Ampicillin; Catalog Number: E1881; GenBank Accession Number:U57026; Comments:CAT reporter; SV40 enhancer located downstream of CAT gene; polyA signal enables ve... |
 | pCAT3-Promoter - Vendor:Promega; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: SV40; Expression Level: High; Sequencing Primer: T7 Fwd; Bacteria Resistance: Ampicillin; Catalog Number: E1861; GenBank Accession Number:U57027; Comments:CAT reporter; SV40 promoter upstream of intron and CAT gene |
 | pcDNA DEST40 - Vendor:Invitrogen; Alternate Vector Names: pDEST40; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: V5; Bacteria Resistance: Ampicillin; Comments:Easy to clone into other vectors |
| Plasmids 30 - 34 | Descriptions |
_thu.jpg) | pcDNA3.1(-) - Vendor:Invitrogen; Alternate Vector Names: pcDNA3.1-, pcDNA3.1; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Bacteria Resistance: Ampicillin; Mammalian Selection: G418, neo; Catalog Number: V79020, V... |
_thu.jpg) | pcDNA3.1(+) - Vendor:Invitrogen; Alternate Vector Names: pcDNA3.1+, pcDNA3.1; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Bacteria Resistance: Ampicilin; Mammalian Selection: G418, neo; Catalog Number: V790-20; C... |
_myc-His_A_thu.jpg) | pcDNA3.1(+)/myc-His A - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, myc; Bacteria Resistance: Ampicillin; Mammalian Selection: G418; Catalog Number: V80020 |
_myc-His_B_thu.jpg) | pcDNA3.1(+)/myc-His B - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, myc; Bacteria Resistance: Ampicillin; Mammalian Selection: G418; Catalog Number: V80020 |
_myc-His_C_thu.jpg) | pcDNA3.1(+)/myc-His C - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, myc; Bacteria Resistance: Ampicillin; Mammalian Selection: G418; Catalog Number: V80020 |
| Plasmids 35 - 39 | Descriptions |
 | pcDNA3.1/CT-GFP Fusion - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: GFP (Cterm); Bacteria Resistance: Ampicillin; Mammalian Selection: G418 |
 | pcDNA3.1/His A - Vendor:Invitrogen; Alternate Vector Names: pcDNA 3.1 His A; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: His; Bacteria Resistance: Ampicillin; Mammalian Selection: neomycin; Catalog Number... |
 | pcDNA3.1/His B - Vendor:Invitrogen; Alternate Vector Names: pcDNA 3.1 His B; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: His; Bacteria Resistance: Ampicillin; Mammalian Selection: neomycin; Catalog Number... |
 | pcDNA3.1/His C - Vendor:Invitrogen; Alternate Vector Names: pcDNA 3.1 His C; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: His; Bacteria Resistance: Ampicillin; Mammalian Selection: neomycin; Catalog Number... |
_thu.jpg) | pcDNA3.1/Hygro(-) - Vendor:Invitrogen; Alternate Vector Names: pcDNA 3.1 Hygro(-); Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Bacteria Resistance: Ampicillin; Mammalian Selection: Hygromycin; Catalog Numbe... |
| Plasmids 40 - 44 | Descriptions |
_thu.jpg) | pcDNA3.1/Hygro(+) - Vendor:Invitrogen; Alternate Vector Names: pcDNA 3.1 Hygro (+); Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Bacteria Resistance: Ampicillin; Mammalian Selection: Hygromycin; Catalog Numb... |
 | pcDNA3.1/NT-GFP Fusion - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: GFP (Nterm); Bacteria Resistance: Ampicillin; Mammalian Selection: G418 |
 | pcDNA3.1/nV5-DEST - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: V5; Bacteria Resistance: Ampicillin; Mammalian Selection: G418; Catalog Number: 12290010; Comments:Easy to clone into other vectors |
_thu.jpg) | pcDNA3.1/Zeo(-) - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: constiutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Bacteria Resistance: Ampicillin; Mammalian Selection: Zeocin; Catalog Number: V86520; Comments:Differs from other pcDNA3.1 in dr... |
_thu.jpg) | pcDNA3.1/Zeo(+) - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Bacteria Resistance: Ampicillin; Mammalian Selection: Zeocin; Catalog Number: V86020; Comments:Differs from other pcDNA3.1 in d... |
| Plasmids 45 - 49 | Descriptions |
 | pcDNA3.1D V5-His-TOPO - Vendor:Invitrogen; Alternate Vector Names: pcDNA 3.1 D V5-His-TOPO; Vector Type: Mammalian Expression; Promoter: CMV; Sequencing Primer: T7; Sequencing Primer Sequence: BGH rev; Tag: C-terminal V5 and His tag; Bacteria Resistance: Ampicillin; Mammalian Selection: Neomycin; Catalog Number: K4900-01 |
 | Vector Backbone: pcDNA3.1/His A -
Vendor: Invitrogen ;
Alternate Vector Names: pcDNA 3.1 His A ;
Vector Type: Mammalian ;
Viral/Non-viral: Nonviral ;
Stable/Transient: Transient ;
Constitutive/Inducible: Constitutive ;
Promoter: CMV ;
Expression Level: High ;
Sequencing Primer: T7 Fwd ;
Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3' ;
Tag: His... |
 | pcDNA4/His A - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, Xpress; Bacteria Resistance: Ampicillin; Mammalian Selection: Zeocin; Catalog Number: V86220; Comments:Easy to clone int... |
 | pcDNA4/His B - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, Xpress; Bacteria Resistance: Ampicillin; Mammalian Selection: Zeocin; Catalog Number: V86220; Comments:Easy to clone int... |
 | pcDNA4/His C - Vendor:Invirtogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, Xpress; Bacteria Resistance: Ampicillin; Mammalian Selection: Zeocin; Catalog Number: V86220; Comments:Easy to clone int... |
| Plasmids 50 - 54 | Descriptions |
 | pcDNA4/His-Max A - Vendor:Invitrogen; Alternate Vector Names: pcDNA4 HisMax; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, Xpress; Bacteria Resistance: Ampicillin; Mammalian Selection: Zeocin; Cat... |
 | pcDNA4/His-Max B - Vendor:Invitrogen; Alternate Vector Names: pcDNA4 HisMax B; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, Xpress; Bacteria Resistance: Ampicillin; Mammalian Selection: Zeocin; C... |
 | pcDNA4/His-Max C - Vendor:Invitrogen; Alternate Vector Names: pcDNA4 HisMax C; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, Xpress; Bacteria Resistance: Ampicillin; Mammalian Selection: Zeocin; C... |
 | pcDNA4/myc-His A - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, myc; Bacteria Resistance: Ampicillin; Mammalian Selection: Zeocin; Catalog Number: V86320 |
 | pcDNA4/myc-His B - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, myc; Bacteria Resistance: Ampicillin; Mammalian Selection: Zeocin; Catalog Number: V86320 |
| Plasmids 55 - 59 | Descriptions |
 | pcDNA4/myc-His C - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, myc; Bacteria Resistance: Ampicillin; Mammalian Selection: Zeocin; Catalog Number: V86320 |
 | pcDNA4/V5-His A - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, V5; Bacteria Resistance: Ampicillin; Mammalian Selection: Zeocin; Catalog Number: V86120; Comments:Easy to clone i... |
 | pcDNA4/V5-His B - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, V5; Bacteria Resistance: Ampicillin; Mammalian Selection: Zeocin; Catalog Number: V86120; Comments:Easy to clone into other vectors. |
 | pcDNA4/V5-His C - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 'd[TAATACGACTCACTATAGGG]3'; Bacteria Resistance: Ampicillin; Mammalian Selection: Zeocin; Catalog Number: V86120; Comments:Easy to clone into other vectors. |
 | pcDNA5/FRT/TO - Vendor:Invitrogen; Vector Type: Mammalian; Bacteria Resistance: Ampicillin |
| Plasmids 60 - 64 | Descriptions |
 | pcDNA5/FRT/TO-E - Vendor:Invitrogen; Vector Type: Mammalian; Bacteria Resistance: Ampicillin |
 | pcDNA5/FRT/TO-TOPO - Vendor:Invitrogen; Vector Type: Mammalian; Bacteria Resistance: Ampicillin |
 | pcDNA5/FRT/V5 His TOPO - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable; Constitutive/Inducible: Constitutive; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, V5; Bacteria Resistance: Ampicillin; Catalog Number: K602001 |
 | pcDNA6.2/V5-DEST - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: V5; Bacteria Resistance: Ampicillin; Mammalian Selection: Blasticidin; Catalog Number: 12489027; Comments:Easy to clone ... |
 | pcDNA6.2/V5-GW/Directional TOPO - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: CMVPro Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: V5; Bacteria Resistance: Ampicillin; Mammalian Selection: Blasticidin or Neomycin (dep... |
| Plasmids 65 - 69 | Descriptions |
 | pcDNA6 BioEase-DEST - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: biotin; Bacteria Resistance: Ampicillin; Mammalian Selection: Blasticidin; Catalog Number: K98001; Comments:Easy to clone into other vectors |
 | pcDNA6/His A - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, Xpress; Bacteria Resistance: Ampicillin; Mammalian Selection: Blasticidin; Catalog Number: V22220 |
 | pcDNA6/His B - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, Xpress; Bacteria Resistance: Ampicillin; Mammalian Selection: Blasticidin; Catalog Number: V22220; Comments:Easy to clon... |
 | pcDNA6/His C - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, Xpress; Bacteria Resistance: Ampicillin; Mammalian Selection: Blasticidin; Catalog Number: V22220; Comments:Easy to clon... |
 | pcDNA6/myc-His A - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, myc; Bacteria Resistance: Ampicillin; Mammalian Selection: Blasticidin; Catalog Number: V22120; Comments:Easy to... |
| Plasmids 70 - 74 | Descriptions |
 | pcDNA6/myc-His B - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Consitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, myc; Bacteria Resistance: Ampicillin; Mammalian Selection: Blasticidin; Catalog Number: V22120; Comments:Easy to ... |
 | pcDNA6/myc-His C - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, myc; Bacteria Resistance: Ampicillin; Mammalian Selection: Blasticidin; Catalog Number: V22120; Comments:Easy to... |
 | pcDNA-DEST47 - Vendor:Invitrogen; Alternate Vector Names: pDEST47; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: GFP (Cterm); Bacteria Resistance: Ampicillin; Mammalian Selection: Neomycin; Catalog Number: 12... |
 | pcDNA-DEST53 - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: GFP (Nterm); Bacteria Resistance: Ampicillin; Mammalian Selection: G418; Catalog Number: 12288-015; Comments:Easy to clone into ... |
 | pCMV/myc/cyto - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: CMVPro Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: myc; Bacteria Resistance: Ampicillin; Mammalian Selection: Neomycin; Catalog Number: V82020; Comments:Targets protein to c... |
| Plasmids 75 - 79 | Descriptions |
 | pCMV/myc/ER - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: CMVPro Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: myc; Bacteria Resistance: Ampicillin; Mammalian Selection: G418; Catalog Number: V82320; Comments:Targets protein to ER |
 | pCMV/myc/mito - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: CMVPro Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: myc; Bacteria Resistance: Ampicillin; Mammalian Selection: G418; Catalog Number: V82220; Comments:Targets protein to mitoc... |
 | pCMV/myc/nuc - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: CMVPro Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: myc; Bacteria Resistance: Ampicillin; Mammalian Selection: G418; Catalog Number: V82120; Comments:Targets protein to nucleus |
 | pCMV-HA - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Sequencing Primer: pCMV; Sequencing Primer Sequence: 5'd[GATCCGGTACTAGAGGAACTGAAA AAC]3'; Tag: HA (Nterm); Bacteria Resistance: Ampicillin; Catalog Number: 631604; Comments:HA tag |
 | pCMV-Myc - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Sequencing Primer: pCMV; Sequencing Primer Sequence: 5'd[GATCCGGTACTAGAGGAACTGAAA AAC]3'; Tag: Myc (Nterm); Bacteria Resistance: Ampicillin; Catalog Number: 631604; Comments:Myc tag |
| Plasmids 80 - 84 | Descriptions |
 | pCMV-Script - Vendor:Stratagene; Vector Type: Bacterial & mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: CMVPro Fwd; Bacteria Resistance: Kanamycin; Mammalian Selection: G418; Catalog Number: 212220; Comments:Can be used in both mammalian and bacterial systems |
 | pCMV-TnT - Vendor:Promega; Alternate Vector Names: pCMVTNT; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: T7 or SP6; Bacteria Resistance: Ampicillin; Catalog Number: L5620; GenBank Accession Number:AF477200; Comments:In vitro & in vivo gene expression |
 | pCMV-VSV-G - Vector backbone: na ;
5' Sequencing primer: T7 ;
Bacteria resistance: Ampicillin ;
High or low copy: High Copy ;
Grow in standard E. coli @ 37C: Yes ;
Plasmid Provided In: DH5a ;
Principal Investigator: Bob Weinberg ;
Comments: VSVG envelope protein, for use with lentiviral and MuLV vectors. ; Article: Lentivirus-delivered stable gene silencing by RNAi in primary ... |
 | pCS2+ - Vendor: RZPD ;
Vector Type: Mammalian expression ;
Promoter: simian CMV IE94 ;
Sequencing Primer: SP6 (5'' of insert) ;
Bacteria Resistance: Ampicillin ;
Comments: pCS2+ contains a strong enhancer/promoter (simian CMV IE94) followed by a polylinker and the SV40 late polyadenlyation site. An SP6 promoter is present in the 5'' untranslated region of the mRNA from the sCMV promoter, all... |
 | pCX4pur - Vector Type: Mammalian; Viral/Non-viral: Retroviral; Bacteria Resistance: Ampicillin; Mammalian Selection: Puromycin; Comments:Akagi,T. et al., Refractory nature of normal human diploid fibroblasts with respect to oncogene-mediated transformation, PNAS 100 (23), 13567-13572 (2003). |
| Plasmids 85 - 89 | Descriptions |
 | pCXneo - Vector Type: Mammalian; Viral/Non-viral: Retroviral; Bacteria Resistance: Ampicillin; Mammalian Selection: Neomycin; Comments:Akagi,T. et al., v-Crk activates the phosphoinositide 3-kinase/AKT pathway in transformation, PNAS 97 (13), 7290-7295 (2000). |
 | pDEST26 - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Sequencing Primer: CMVPro Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His; Bacteria Resistance: Ampicillin; Mammalian Selection: G418; Catalog Number: 11809019; Comments:Easy to clone into other vectors |
 | pDEST27 - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Sequencing Primer: CMVPro Fwd; Sequencing Primer Sequence: 5'd[CGCAAATGGGCGGTAGGCGTG]3'; Tag: GST; Bacteria Resistance: Ampicillin; Mammalian Selection: G418; Catalog Number: 11812013; Comments:Easy to clone into other vectors |
 | pDisplay - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Bacteria Resistance: Ampicillin; Mammalian Selection: G418; Catalog Number: V66020; Comments:Targest protein to cell surface |
 | pDNR-CMV - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Sequencing Primer: T7; Bacteria Resistance: Ampicillin; Catalog Number: 639602; Comments:Donor plasmid with loxP sites flanking gene and CMV promoter. For use with cre recombinase. |
| Plasmids 90 - 94 | Descriptions |
 | pDRIVE-CAG - Vendor:Invivogen; Vector Type: Mammalian; Promoter: CAG; Bacteria Resistance: Zeocin |
 | pDsRed2-1 - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: Constitutive; Promoter: none; Sequencing Primer: DsRed1-N; Sequencing Primer Sequence: 5'd[GTACTGGAACTGGGGGGACAG]; Bacteria Resistance: Kanamycin; Mammalian Selection: Neomycin; Catalog Number: 632405; Comments:Red fluorescent protein reporter. Used to monitor transc... |
 | pDsRed2-C1 - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: Constitutive; Promoter: CMV; Sequencing Primer: DsRed1-C; Sequencing Primer Sequence: 5'd[AGCTGGACATCACCTCCCACAACG]; Tag: DsRed2 (Nterm); Bacteria Resistance: Kanamycin; Mammalian Selection: Neomycin; Catalog Number: 632407; Comments:Red fluorescent protein tag |
 | pDsRed2-N1 - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: Constitutive; Promoter: CMV; Sequencing Primer: DsRed; Sequencing Primer Sequence: 5'd[GTACTGGAACTGGGGGGACAG]; Tag: DsRed2 (Cterm); Bacteria Resistance: Kanamycin; Mammalian Selection: Neomycin; Catalog Number: 632406; Comments:Red fluorescent protein tag |
 | pDsRed-Express-1 - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: No promoter; Promoter: none; Sequencing Primer: DsRed; Sequencing Primer Sequence: 5'd[GTACTGGAACTGGGGGGACAG]; Bacteria Resistance: Kanamycin; Mammalian Selection: Neomycin; Catalog Number: 632413; Comments:Red fluorescent protein reporter. Used to moni... |
| Plasmids 95 - 99 | Descriptions |
 | pDsRed-Express-C1 - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: Constitutive; Promoter: CMV; Sequencing Primer: DsRed1-C; Sequencing Primer Sequence: 5'd[AGCTGGACATCACCTCCCACAACG]; Tag: DsRed-Express (Nterm); Bacteria Resistance: Kanamycin; Mammalian Selection: Neomycin; Catalog Number: 632430; Comments:Red fluore... |
 | pDsREd-Express-DR - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: No promoter; Promoter: none; Sequencing Primer: DsRed; Sequencing Primer Sequence: 5'd[GTACTGGAACTGGGGGGACAG]; Bacteria Resistance: Kanamycin; Mammalian Selection: Neomycin; Catalog Number: 632423; Comments:Red fluorescent protein (shorter half-life) ... |
 | pDsRed-Express-N1 - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: Constitutive; Promoter: CMV; Sequencing Primer: DsRed1-N; Sequencing Primer Sequence: 5'd[GTACTGGAACTGGGGGGACAG]; Tag: DsRed-Express (Cterm); Bacteria Resistance: Kanamycin; Mammalian Selection: Neomycin; Catalog Number: 632429; Comments:Red fluoresce... |
 | pECFP-1 - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: No promoter; Promoter: none; Sequencing Primer: EGFP-N; Sequencing Primer Sequence: 5'd[CGTCGCCGTCCAGCTCGACCAG]3'; Bacteria Resistance: Kanamycin; Mammalian Selection: Neomycin; Catalog Number: 6901-1; Comments:Cyan variant of GFP reporter. Used to monitor transcription ... |
 | pECFP-C1 - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: Constitutive; Promoter: CMV; Sequencing Primer: EGFP-C; Sequencing Primer Sequence: 5'd[CATGGTCCTGCTGGAGTTCGTG]; Tag: ECFP (Nterm); Bacteria Resistance: Kanamycin; Mammalian Selection: Neomycin; Catalog Number: 6076-1; Comments:Cyan variant of GFP tag |
| Plasmids 100 - 104 | Descriptions |
 | pECFP-N1 - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: Constitutive; Promoter: CMV; Sequencing Primer: EGFP-N; Sequencing Primer Sequence: 5'd[CGTCGCCGTCCAGCTCGACCAG]3'; Tag: ECFP (Cterm); Bacteria Resistance: Kanamycin; Mammalian Selection: Neomycin; Catalog Number: 6900-1; Comments:Cyan variant of GFP tag |
 | pEF1/His A - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: EF-1a; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, Xpress; Bacteria Resistance: Ampicillin; Mammalian Selection: G418; Catalog Number: V92220; Comments:Easy to clone into ot... |
 | pEF1/His B - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: EF-1a; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, Xpress; Bacteria Resistance: Ampicillin; Mammalian Selection: G418; Catalog Number: V92220; Comments:Easy to clone into ot... |
 | pEF1/His C - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: EF-1a; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, Xpress; Bacteria Resistance: Ampicillin; Mammalian Selection: G418; Catalog Number: V92220; Comments:Easy to clone into ot... |
 | pEF1/myc-His A - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: EF-1a; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, myc; Bacteria Resistance: Ampicillin; Mammalian Selection: G418; Catalog Number: V92120; Comments:Easy to clone in... |
| Plasmids 105 - 109 | Descriptions |
 | pEF1/myc-His B - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: EF-1a; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, myc; Bacteria Resistance: Ampicillin; Mammalian Selection: G418; Catalog Number: V92120; Comments:Easy to clone in... |
 | pEF1/myc-His C - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: EF-1a; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, myc; Bacteria Resistance: Ampicillin; Mammalian Selection: G418; Catalog Number: V92120; Comments:Easy to clone in... |
 | pEF1/V5 His A - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: EF-1a; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, V5; Bacteria Resistance: Ampicillin; Mammalian Selection: G418; Catalog Number: V92020; Comments:Easy to clone into ... |
 | pEF1/V5 His B - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: EF-1a; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, V5; Bacteria Resistance: Ampicillin; Mammalian Selection: G418; Catalog Number: V92020; Comments:Easy to clone into ... |
 | pEF1/V5 His C - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: EF-1a; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, V5; Bacteria Resistance: Ampicillin; Mammalian Selection: G418; Catalog Number: V92020; Comments:Easy to clone into ... |
| Plasmids 110 - 114 | Descriptions |
 | pEF4/His A - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: EF-1a; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, Xpress; Bacteria Resistance: Ampicillin; Mammalian Selection: Zeocin; Catalog Number: V94320; Comments:Easy to clone into ... |
 | pEF4/His B - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: EF-1a; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, Xpress; Bacteria Resistance: Ampicillin; Mammalian Selection: Zeocin; Catalog Number: V94320; Comments:Easy to clone into ... |
 | pEF4/His C - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: EF-1a; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, Xpress; Bacteria Resistance: Ampicillin; Mammalian Selection: Zeocin; Catalog Number: V94320; Comments:Easy to clone into ... |
 | pEF4/myc-His A - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: EF-1a; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, myc; Bacteria Resistance: Ampicillin; Mammalian Selection: Zeocin; Catalog Number: V94220; Comments:Easy to clone ... |
 | pEF4/myc-His B - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: EF-1a; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, myc; Bacteria Resistance: Ampicillin; Mammalian Selection: Zeocin; Catalog Number: V94220; Comments:Easy to clone ... |
| Plasmids 115 - 119 | Descriptions |
 | pEF4/myc-His C - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: EF-1a; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, myc; Bacteria Resistance: Ampicillin; Mammalian Selection: Zeocin; Catalog Number: V94220; Comments:Easy to clone ... |
 | pEF4/V5 His A - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: EF-1a; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, V5; Bacteria Resistance: Ampicillin; Mammalian Selection: Zeocin; Catalog Number: V94120; Comments:Easy to clone int... |
 | pEF4/V5 His B - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: EF-1a; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, V5; Bacteria Resistance: Ampicillin; Mammalian Selection: Zeocin; Catalog Number: V94120; Comments:Easy to clone int... |
 | pEF4/V5 His C - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: EF-1a; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, V5; Bacteria Resistance: Ampicillin; Mammalian Selection: Zeocin; Catalog Number: V94120; Comments:Easy to clone int... |
 | pEF5/FRT/V5 DEST Gateway - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable; Constitutive/Inducible: Constitutive; Promoter: EF-1a; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: V5; Bacteria Resistance: Ampicillin; Mammalian Selection: Hygromycin; Catalog Number: V6020-20 |
| Plasmids 120 - 124 | Descriptions |
 | pEF5/FRT/V5 D-TOPO - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable; Constitutive/Inducible: Constitutive; Promoter: EF-1a; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: V5; Bacteria Resistance: Ampicillin; Mammalian Selection: Hygromycin; Catalog Number: K6035-01 |
 | pEF5/FRT/V5 TOPO - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable; Constitutive/Inducible: Constitutive; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: V5; Bacteria Resistance: Ampicillin; Mammalian Selection: Hygromycin; Catalog Number: K603501 |
 | pEF6/His A - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: EF-1a; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His; Bacteria Resistance: Ampicillin; Mammalian Selection: G418, zeocin, blasticidin; Catalog Number: V96120 |
 | pEF6/His B - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: EF-1a; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His; Bacteria Resistance: Ampicillin; Mammalian Selection: G418, zeocin, blasticidin; Catalog Number: V96120 |
 | pEF6/His C - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: EF-1a; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: +5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His; Bacteria Resistance: Ampicillin; Mammalian Selection: G418, zeocin, blasticidin; Catalog Number: V96120 |
| Plasmids 125 - 129 | Descriptions |
 | pEF6/myc-His A - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: EF-1a; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, myc; Bacteria Resistance: Ampicillin; Mammalian Selection: G418, zeocin, blasticidin; Catalog Number: V96220; Comm... |
 | pEF6/myc-His B - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: EF-1a; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, myc; Bacteria Resistance: Ampicillin; Mammalian Selection: G418, zeocin, blasticidin; Catalog Number: V96220; Comm... |
 | pEF6/myc-His C - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: EF-1a; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, myc; Bacteria Resistance: Ampicillin; Mammalian Selection: G418, zeocin, blasticidin; Catalog Number: V96220; Comm... |
 | pEF6/V5 His TOPO - Vendor:Invitrogen; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: EF-1a; Expression Level: High; Sequencing Primer: T7 Fwd; Sequencing Primer Sequence: 5'd[TAATACGACTCACTATAGGG]3'; Tag: 6X His, V5; Bacteria Resistance: Ampicillin; Mammalian Selection: Blasticidin; Catalog Number: K961020; Comments:Easy ... |
 | pEF-GFP - Gene/insert name: EF1 alpha promoter ;
Alternative names: EF1A1 promoter ;
Gene/insert aliases: EEF1A1, CCS3, EF1A, PTI1, CCS-3, EEF-1, EEF1A, EF-Tu, LENG7, eEF1A-1, FLJ25721, GRAF-1EF, MGC16224, MGC102687, MGC131894, HNGC:16303
Species of gene(s): H. sapiens (human);
Fusion proteins or tags: EGFP ;
Terminal: C terminal on backbone ;
Vector backbone: pCAGEN ;
Clo... |
| Plasmids 130 - 134 | Descriptions |
 | pEGFP-1 - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: No promoter; Promoter: none; Sequencing Primer: EGFP-N; Sequencing Primer Sequence: 5'd[CGTCGCCGTCCAGCTCGACCAG]3'; Bacteria Resistance: Kanamycin; Mammalian Selection: Neomycin; Catalog Number: 6086-1; Comments:EGFP reporter. Used to monitor transcription on cis-regulato... |
 | pEGFP-C1 - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: Constitutive; Promoter: CMV; Sequencing Primer: EGFP-C; Sequencing Primer Sequence: 5'd[CATGGTCCTGCTGGAGTTCGTG]; Tag: EGFP (Nterm); Bacteria Resistance: Kanamycin; Mammalian Selection: Neomycin; Catalog Number: 6084-1; Comments:EGFP tag |
 | pEGFP-C3 - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: Constitutive; Promoter: CMV; Sequencing Primer: EGFP-C; Sequencing Primer Sequence: 5'd[CATGGTCCTGCTGGAGTTCGTG]; Tag: EGFP (Nterm); Bacteria Resistance: Kanamycin; Mammalian Selection: Neomycin; Catalog Number: 6082-1; Comments:EGFP tag |
 | pEGFP-N1 - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: Constitutive; Promoter: CMV; Sequencing Primer: CMV-F, EGFP-N; Sequencing Primer Sequence: 5'd[CGTCGCCGTCCAGCTCGACCAG]3'; Tag: EGFP (Cterm); Bacteria Resistance: Kanamycin; Mammalian Selection: Neomycin; Catalog Number: 6085-1; Comments:EGFP tag |
 | pEGFP-N3 - Vendor:Clontech; Vector Type: Mammalian; Viral/Non-viral: Nonviral; Stable/Transient: Stable (transfected); Constitutive/Inducible: Constitutive; Promoter: CMV; Sequencing Primer: EGFP-N; Sequencing Primer Sequence: 5'd[CGTCGCCGTCCAGCTCGACCAG]3'; Tag: EGFP (Cterm); Bacteria Resistance: Kanamycin; Mammalian Selection: Neomycin; Catalog Number: 6080-1; Comments:EGFP tag |
| Plasmids 135 - 139 | Descriptions |
 | Vector Backbone: pIRESneo3 -
Vendor: Clontech ;
Alternate Vector Names: pIRES neo 3 ;
Vector Type: mammalian expression ;
Constitutive/Inducible: constitutive ;
Promoter: CMV ;
Tag: IRES-neo on transcript ;
Bacteria Resistance: ampicillin ;
Mammalian Selection: neomycin ;
Catalog Number: 6988-1 |
 | pMD2.G - Gene/insert name: VSV G; Species of gene(s): Other; Vector backbone: pMD2.G (Search Vector Database); 5' Sequencing primer: CMV Fwd (List of Sequencing Primers); Bacteria resistance: Ampicillin; High or low copy: High Copy; Grow in standard E. coli @ 37C: Yes; Plasmid Provided In: DH5a; Principal Investigator: Didier Trono; Comments: Envelope plasmid. Known to work with most Aebischer a... |
 | pRK7 - Vector backbone: pRK7 ;
5' Sequencing primer: SP6 ;
3' Sequencing primer: pSG5-3' ;
Bacteria resistance: Ampicillin ;
High or low copy: High Copy ;
Grow in standard E. coli @ 37C: Yes ;
Plasmid Provided In: DH5a ;
Principal Investigator: John Blenis ;
Comments: Vector origin unknown (not created by the Blenis lab). |
 | pST1374 - Gene/insert name: none; Fusion proteins or tags: Flag; Terminal: N terminal on backbone; Vector backbone: pST1374 (Search Vector Database); Cloning site 5': XbaI; Site destroyed during cloning: No; Cloning site 3': BamHI; Site destroyed during cloning: No; 5' Sequencing primer: T7 (List of Sequencing Primers); Bacteria resistance: Ampicillin; High or low copy: High Copy; Grow ... |
|
|
|
|
Copyright 2005 BVTech, Inc. All Rights Reserved
|
|
|
|
|
|
Search plasmid maps in this site
|
|
|
|
|
More Plasmid Map Templates
|
|
|
|
|
|
|
