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pMN19
Gene/insert name: CaMV 35S enhancers
Insert size (bp): Unknown
Vector backbone: pPZP212
Backbone manufacturer: Hajdukiewicz et al 1994
Type of vector: Plant expression
Backbone size (bp): 11073
Cloning site 5':
Site destroyed during cloning: No
Cloning site 3': See paper and map.
Site destroyed during cloning: No
5' Sequencing primer: M13 reverse
Bacteria resistance: Spectinomycin
High or low copy: High Copy
Grow in standard E. coli @ 37C: No
Please specify bacterial strain for growth and growth condition: Plasmid grow in standard E. coli at 37 degrees, but the four enhancer repeats may be unstable in E. coli and A. tumefaciens if stored at 4'C for extended time. See comments.
Selectable markers: Neomycin
If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid: Max Planck Institute
Plasmid Provided In: DH5a
Principal Investigator: Detlef Weigel
Comments: Used to confirm activation-tagged genes. Contains multiple cloning sites adjacent to the same tetramerized CaMV 35S enhancers as in the activation-tagging vectors from this article.
Kanamycin gene for plant selection on MS media.
Plasmid selection in E. coli and in A. tumefaciens is spectinomycin (100 mg/l).
Article: Activation tagging in Arabidopsis. Weigel D et al. (Plant Physiol. 2000 Apr . 122(4):1003-13. Pubmed)
V143 pLP-HA SD - Acceptor
Relevant mutations/deletions: Splice for 5' tags
Fusion proteins or tags: HA
Terminal: N terminal on backbone
Vector backbone: NA
Type of vector: Mammalian expression,Cre/Lox,Acceptor vector
Backbone size (bp): 4519
5' Sequencing primer: CMV-F
Bacteria resistance: Kanamycin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Plasmid Provided In: DH5a
Principal Investigator: Tony Pawson
Comments: Acceptor vector for the Creator Splice system.
Article: Modification of the Creator recombination system for proteomics applications--improved expression by addition of splice
pLI50
Type of vector: S. aureus-E. coli shuttle vector
Backbone size (bp): 5505
5' Sequencing primer: N/A
Bacteria resistance: Ampicillin
High or low copy: Don't Know
Grow in standard E. coli @ 37C: Yes
Selectable markers: Chloramphenicol in S. aureus at 37C
Plasmid Provided In: DH5a
Principal Investigator: Chia Y. Lee
Article: Construction of single-copy integration vectors for Staphylococcus aureus. Lee CY et al. (Gene. 1991 Jul 15. 103(1):101-5.
Pubmed)
pDW1775
Gene/insert name: none
Insert size (bp): Unknown
Fusion proteins or tags: AcV5
Terminal: N terminal on backbone
Vector backbone: pDW1775
Type of vector: Plant expression
Backbone size (bp): 5528
Cloning site 5': XbaI
Site destroyed during cloning: No
Cloning site 3': BamHI
Site destroyed during cloning: No
5' Sequencing primer: T7
3' Sequencing primer: T3
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Plasmid Provided In: DH5a
Principal Investigator: Daniel Voytas
Comments: Plant zinc finger nuclease cloning vector. Zinc fingers are cloned in between unique XbaI and BamHI sites in front of the FokI nuclease domain.
pDW1775 consists of the following components in the given order: Hinc II, SexA I, EcoN I, lambda T0 terminator, T7 primer site, BsiW I, rrnB terminator, modified NOS promoter, Bgl II, Xho I, plant leader, MASS translation signal, SV40 nuclear localization signal, AcV5 epitope tag, Xba I, Xma I, Age I, BamH I, plant optimized Fok I nuclease domain, Sac I, NOS terminator, Apa I, T3 primer site, phage FD gene VIII terminator, Bpu 10 I, modified yeast His3 gene, Pac I, yeast CEN/ARS, Bsu36 I (not unique), modified beta lactamase gene (ampicillin resistance), E. coli Trp A terminator, BstE II, Blp I, PpuM I, Rsr II, PflF I, Bgl I, modified high copy ColEI replicon, and back to Hinc II. This vector can be maintained in E. coli and yeast (Saccharomyces cerevisiae) and is also able to express a zinc finger nuclease in yeast and plants using the modified NOS promoter to drive expression. Additionally, this vector was designed to have minimal expression of the zinc finger nuclease in E. coli to facilitate cloning and reduce toxicity effects.
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