Other Plasmids
6xHisGFP to BlueScribe KS - | Bluescribe KS + to BlueScribe SK + | BPV-1 to Carnegie 1 | Carnegie 2 to EMBL3 SP6/T7 left arm | EMBL3 SP6/T7 right arm to LoristE6 | pACYC184 to pBK-RSV | pCMV-Sport_1 to pENTR_H1_TO | pENTR_L1-pLac-LacZalpha-R5 to pENTR_U6 | pENTR1A to pET3a | pET3b to pET3xb | pET3xc to pFastBac1 | pFLD to pGFPTA | pGL4.26[luc2_minP_Hygro] to pT7T3-18 | pT7T3-18U to V143 pLP-HA SD - Acceptor |
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| Plasmids 0 - 4 | Descriptions |
 | 6xHisGFP - Vendor: Clontech ;
GenBank Accession Number: U89936 ;
Type of vector: Other |
 | Bacteriophage fd strain 478 - GenBank Accession Number: M25198; Hosts: E.coli. Related vectors: fd. (Information source: VectorDB.); Type of Vector: Other |
 | Beta-actin GFP -
Alternate Vector Names: AY952326 ;
Type of vector: Other |
 | Bluescribe -
GenBank Accession Number: M77811 ;
Type of Vector: Other |
 | BlueScribe KS - - GenBank Accession Number: L08784 |
| Plasmids 10 - 14 | Descriptions |
 | BPV-1 - GenBank Accession Number: X02346 M24622 X00473; Hosts: cow. Related vectors: cow. (Information source: VectorDB.); Type of Vector: Other |
 | BSB- - Vendor: ATCC; Catalog Number: 67725; Comments: Created by Moore, July 1995, under contract with NCBI. Constructed from Bluescribe M13- by insertion of a synthetic BstXI adapter between the PstI & XbaI sites of the polylinker region. A plasmid vector containing convenient priming sites for sequence analysis and synthesis of RNA in either direction from T3 and T7 promoters. BSB+ (away from cloning sit... |
 | BSB+ - Vendor: ATCC; Catalog Number: 67724; Comments: Created by Moore, July 1995, under contract with NCBI. BSB+ (away from cloning site) and BSB- (toward cloning site) differ in their orientation of the intergenic region from bacteriophage f1. This vector contains the integenic region from bacteriophage f1 permitting synthesis of single-strand copies of the insert. A plasmid vector containing convenient p... |
 | BSII TKS- - Comments: Created by Moore, July 1995, under contract with NCBI.; Hosts: E.coli. Related vectors: pBluescript II KS-. (Information source: VectorDB.); Type of Vector: Other |
 | Carnegie 1 - Vendor: ATCC; Catalog Number: 37238; GenBank Accession Number: M30841; Comments: This is a plasmid vector containing a non-autonomous P element from Drosophila melanogaster. It should be used with a helper plasmid such as ppi25.1 (ATCC 37236) or ppi25.7 (ATCC 37237). Medium is 1227 LB plus ampicillin.; Hosts: E.coli K-12, Drosophila melanogaster, E.coli.; Related vectors: pUC8, Drosophila P ... |
| Plasmids 15 - 19 | Descriptions |
 | Carnegie 2 - Vendor: ATCC; Catalog Number: 37239; GenBank Accession Number: M30841; Comments: Created by Moore, July 1995, under contract with NCBI. This is a plasmid vector containing a non-autonomous P element from Drosophila melanogaster. It should be used with a helper plasmid such as ppi25.1 (ATCC 37236) or ppi25.7 (ATCC 37237). Medium is 1227 LB plus ampicillin.; Hosts: E.coli K-12, Drosophila mela... |
 | Carnegie 3 - Vendor: ATCC; Catalog Number: 37240; GenBank Accession Number: M30841; Comments: Created by Moore, July 1995, under contract with NCBI. This is a plasmid vector containing a non-autonomous P element from Drosophila melanogaster. It should be used with a helper plasmid such as ppi25.1 (ATCC 37236) or ppi25.7 (ATCC 37237). Medium is 1227 LB plus ampicillin.; Hosts: E.coli K-12, Drosophila mela... |
 | Carnegie 4 - Vendor: ATCC; Catalog Number: 37241; GenBank Accession Number: M30841; Comments: Created by Moore, July 1995, under contract with NCBI. This is a plasmid vector containing a non-autonomous P element from Drosophila melanogaster. It should be used with a helper plasmid such as ppi25.1 (ATCC 37236) or ppi25.7 (ATCC 37237). Medium is 1227 LB plus ampicillin.; Hosts: E.coli K-12, Drosophila mela... |
 | ColE1/Colicin E1 - Vendor: Sigma; Catalog Number: COLE1; GenBank Accession Number: M33100; Comments: ColE1 plasmid makes Colicin E1, which inhibits other E. coli strains. Cells with the plasmid have immunity to Colicin E1. This is the L (light) strand. The open reading frames noted by [8] are named in FEATURES by the molecular mass of their predicted products or by the names given by [8].; Hosts: E... |
 | EMBL3 SP6/T7 left arm - GenBank Accession Number: U02426; Hosts: E.coli. Related vectors: EMBL3. (Information source: VectorDB.); Type of Vector: Other |
| Plasmids 20 - 24 | Descriptions |
 | EMBL3 SP6/T7 right arm - GenBank Accession Number: U02427; Hosts: E.coli. Related vectors: EMBL3. (Information source: VectorDB.); Type of Vector: Other |
 | fd-tet - Vendor: ATCC; Catalog Number: 37000; Comments: Created by Moore, July 1995, under contract with NCBI. Has been used as the starting material to construct a vector expressing immunoglobulin variable region-gene III fusion proteins which can be screened by antigen with the intact bacteriophage (phage antibodies). [3] Distributed as infected E.coli JM101 (ATCC 33876). (ATCC staff) Medium is 1273 LB plu... |
 | fKN 16 - Vendor: ATCC; Catalog Number: 37002; Comments: Created by Moore, July 1995, under contract with NCBI. This vector contains a deletion in the phage gene III. It exhibits a higher level of biological containment than fd-tet (ATCC 37000). (ATCC staff) fd bacteriophage vector. (ATCC staff) Medium is 1592 SM buffer.; Hosts: bacteriophage fd, E.coli K802, E.coli C600, E.coli.; Related vectors: fd-tet. (Inf... |
 | HaloTag pHT2 - Vendor: Promega; Bacteria Resistance: Ampicillin; Catalog Number: G8241; GenBank Accession Number: AY773970; Type of Vector: Other |
 | LoristE6 - Vendor:ATCC; Catalog Number: 37532; Comments:Created by Moore, July 1995, under contract with NCBI. Accepts blunt-ended DNA. Cosmid vector for genomic library construction. Insert-end-specific probes may be generated from T7 and SP6 promoters. Restriction digests of the clone give the following sizes (kb): BamHI--5.7; HindIII--5.7. (ATCC staff) Medium is 1236 LB plus kanamycin. Hosts: E.coli 1046, ... |
| Plasmids 25 - 29 | Descriptions |
 | pACYC184 - Vendor:ATCC; Catalog Number: 37033; GenBank Accession Number:X06403; Comments:The sequence is circular; however, the sequence begins with a unique EcoRI site. Bases 426-706, 1153-1583, and 2918 to 3735 were determined while the rest of the sequence was taken from the published sequence of pSC101, Tn9, and p15A. Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; EcoRI/BamHI-... |
 | pAMP1 - Vendor:BRL; Comments:Created by Moore, July 1995, under contract with NCBI. Hosts: E.coli. Related vectors: pSPORT1. (Information source: VectorDB.) |
 | pBG1 - Vendor:ATCC; Catalog Number: 87035; Comments:Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.2, 0.55; DraI/EcoRI--2.5, 2.4, 0.7; HindIII--3.5, 2.2; PstI--5.7. (ATCC staff) The his3 insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.49; ClaI--0.04; EcoRI--0.47, 1.01; HindIII--1.03; PvuI--0.04; XbaI--0.27; XhoI--0.84. [1] Growth: LB plus a... |
 | pBK-CMV - Vendor:Stratagene; Comments:Hosts: E.coli XL-1-Blue MRF'. Related vectors: pBR322, pBK-RSV. (Information source: VectorDB.) |
 | pBK-RSV - Vendor:Stratagene; Catalog Number: Discontinued; Comments:Hosts: E.coli XL-1-Blue MRF'. Related vectors: pBR322, pBK-CMV. (Information source: VectorDB.) |
| Plasmids 30 - 34 | Descriptions |
 | pCMV-Sport 1 - Vendor:Invitrogen; Viral/Non-viral: Nonviral; Stable/Transient: Transient; Constitutive/Inducible: Constitutive; Promoter: CMV; Expression Level: High; Sequencing Primer: SP6; Tag: none; Bacteria Resistance: Ampicillin; Mammalian Selection: None |
 | pDNR-1r - Vendor:Clontech; Sequencing Primer: M13; Bacteria Resistance: Ampicillin; Catalog Number: 631615; Comments:Donor plasmid with loxP sites flanking gene. For use with cre-recombinase. sold as a component in the Creator pDNR Cloning kit |
 | pDW1775 - Gene/insert name: none ;
Fusion proteins or tags: AcV5;
Terminal: N terminal on backbone ;
Vector backbone: pDW1775 ;
Cloning site 5': XbaI ;
Site destroyed during cloning: No ;
Cloning site 3': BamHI ;
Site destroyed during cloning: No ;
5' Sequencing primer: T7 ;
3' Sequencing primer: T3 ;
Bacteria resistance: Ampicillin ;
High or low copy: ... |
 | pENTR/D-TOPO - Vendor:Invitrogen; Vector Type: Entry vector; Bacteria Resistance: Kanamycin; Catalog Number: K2400-20; Comments:Directional TOPO cloning vector. |
 | pENTR/H1/TO - Vendor:Invitrogen; Vector Type: Entry vector; Promoter: Human HI/TO; Expression Level: High; Sequencing Primer: M13 forward and reverse; Bacteria Resistance: Kanamycin; Mammalian Selection: Zeocin; Catalog Number: K4920-00; Comments:Contains a hybrid promoter consisting of human H1 promoter and two tetracycline operator sequence for RNA Polymerase III-dependant, regulated expression of the sh... |
| Plasmids 35 - 39 | Descriptions |
 | pENTR L1-pLac-LacZalpha-R5 - Vendor:Invitrogen; Sequencing Primer: M13 F and R; Bacteria Resistance: Kanamycin; Catalog Number: 1237-102 |
 | pENTR L5-pLac-Spect-L2 - Vendor:Invitrogen; Vector Type: Entry vector; Sequencing Primer: M13 F and R; Bacteria Resistance: Kanamycin; Catalog Number: 12357-102 |
 | pENTR/SD/D-TOPO - Vendor:Invitrogen; Vector Type: Entry Vector; Bacteria Resistance: Kanamycin; Catalog Number: K2420-20; Comments:Contains a T7 gene 10 translational enhancer and a ribosonal binding site (RBS) for optimal expression of native protein after recombination with a prokaryotic Gateway destination vector. |
 | pENTR/TEV/D-TOPO - Vendor:Invitrogen; Vector Type: Entry Vector; Bacteria Resistance: Kanamycin; Catalog Number: K252520; Comments:Contains a Tobacco Etch Virus (TEV) recognition site for efficient TEV protease dependant cleavage of an N-terminal tag from your recombinat protein after recombination and expression from a Gateway desitnation vector |
 | pENTR/U6 - Vendor:Invitrogen; Promoter: Human U6; Sequencing Primer Sequence: M13 forward and reverse; Bacteria Resistance: Kanamycin; Catalog Number: K4945-00; Comments:U6 promoter allows for the RNA polymerase III-dependant expression of short hairpin RNA (shRNA). |
| Plasmids 40 - 44 | Descriptions |
 | pENTR1A - Vendor:Invitrogen; Alternate Vector Names: pENTR 1A; Vector Type: Entry Vector; Bacteria Resistance: Kanamycin; Catalog Number: 11813-011; Comments:Gateway Entry vector, ccdB gene (if present) requires DB3.1 E. coli. Produces in-frame (rf=0) expression-ready Entry clones. |
 | pENTR2B - Vendor:Invitrogen; Alternate Vector Names: pENTR 2B; Vector Type: Entry Vector; Bacteria Resistance: Kanamycin; Catalog Number: 11816-014; Comments:Gateway Entry vector, ccdB gene (if present) requires DB3.1 E. coli. Produces in-frame (rf=1) expression-ready Entry clones. |
 | pENTR3C - Vendor:Invitrogen; Alternate Vector Names: pENTR 3C; Vector Type: Entry Vector; Bacteria Resistance: Kanamycin; Catalog Number: 11817-012; Comments:Gateway Entry vector, ccdB gene (if present) requires DB3.1 E. coli. Produces in-frame (rf=2) expression-ready Entry clones. |
 | pENTR4 - Vendor:Invitrogen; Alternate Vector Names: pENTR 4; Vector Type: Entry Vector; Bacteria Resistance: Kanamycin; Catalog Number: 11818-010; Comments:Gateway Entry vector, ccdB gene (if present) requires DB3.1 E. coli. Same as pENTR1A except with NcoI instead of DraI in MCS. |
 | pET3a - Vendor:New England Biolabs; Comments:Hosts: E.coli. Related vectors: pBR322. (Information source: VectorDB.) |
| Plasmids 45 - 49 | Descriptions |
 | pET3b - Vendor:New England Biolabs; Comments:Hosts: E.coli. Related vectors: pBR322. (Information source: VectorDB.) |
 | pET3c - Vendor:New England Biolabs; GenBank Accession Number:M73804; Comments:Hosts: E.coli. Related vectors: pBR322. (Information source: VectorDB.) |
 | pET3d - Vendor:New England Biolabs; Comments:Hosts: E.coli. Related vectors: pBR322. (Information source: VectorDB.) |
 | pET3xa - Vendor:New England Biolabs; Comments:Hosts: E.coli. Related vectors: pBR322. (Information source: VectorDB.) |
 | pET3xb - Vendor:New England Biolabs; Comments:Hosts: E.coli. Related vectors: pBR322. (Information source: VectorDB.) |
| Plasmids 50 - 54 | Descriptions |
 | pET3xc - Vendor:New England Biolabs; Comments:Hosts: E.coli. Related vectors: pBR322. (Information source: VectorDB.) |
 | pET5a - Vendor:New England Biolabs; Comments:Hosts: E.coli. Related vectors: pBR322. (Information source: VectorDB.) |
 | pET5b - Vendor:New England Biolabs; Comments:Hosts: E.coli. Related vectors: pBR322. (Information source: VectorDB.) |
 | pET5c - Vendor:New England Biolabs; Comments:Hosts: E.coli. Related vectors: pBR322. (Information source: VectorDB.) |
 | pFastBac1 - Vendor:Invitrogen; Vector Type: Baculoviral; Promoter: Polyhedrin; Sequencing Primer: polyhedrin forward; Bacteria Resistance: Ampicillin; Mammalian Selection: Gentamicin; Catalog Number: 10359016; Comments:Hosts: E.coli. Related vectors: baculovirus. (Information source: VectorDB.) |
| Plasmids 55 - 59 | Descriptions |
 | pFLD - Vendor:Invitrogen; Vector Type: Pichia pastoris; Viral/Non-viral: Nonviral; Stable/Transient: Stable; Constitutive/Inducible: Inducible (methylamine); Promoter: P-FLD1; Sequencing Primer: 3'AOX1; Sequencing Primer Sequence: 5'd[GCAAATGGCATTCTGACATCC]3'; Tag: V5 (Cterm), His (Cterm); Bacteria Resistance: Ampicillin; Mammalian Selection: Zeocin; Catalog Number: V23020; Comments:Methylamine-inducible expressi... |
 | pGAPZ A - Vendor:Invitrogen; Vector Type: Pichia pastoris; Viral/Non-viral: Nonviral; Stable/Transient: Stable; Constitutive/Inducible: Constitutive; Promoter: Glyceraldehyde-3-phosphate (Pgap); Sequencing Primer: 3'AOX1; Sequencing Primer Sequence: 5'd[GCAAATGGCATTCTGACATCC]3'; Tag: Myc (Cterm), His (Cterm); Bacteria Resistance: ?; Mammalian Selection: Zeocin; Catalog Number: V20020; Comments:Expression and p... |
 | pGAPZ B - Vendor:Invitrogen; Vector Type: Pichia pastoris; Viral/Non-viral: Nonviral; Stable/Transient: Stable; Constitutive/Inducible: Constitutive; Promoter: Glyceraldehyde-3-phosphate (Pgap); Sequencing Primer: 3'AOX1; Sequencing Primer Sequence: 5'd[GCAAATGGCATTCTGACATCC]3'; Tag: Myc (Cterm), His (Cterm); Bacteria Resistance: ?; Mammalian Selection: Zeocin; Catalog Number: V20020; Comments:Expression and p... |
 | pGAPZ C - Vendor:Invitrogen; Vector Type: Pichia pastoris; Viral/Non-viral: Nonviral; Stable/Transient: Stable; Constitutive/Inducible: Constitutive; Promoter: Glyceraldehyde-3-phosphate (Pgap); Sequencing Primer: 3'AOX1; Sequencing Primer Sequence: 5'd[GCAAATGGCATTCTGACATCC]3'; Tag: Myc (Cterm), His (Cterm); Bacteria Resistance: ?; Mammalian Selection: Zeocin; Catalog Number: V20020; Comments:Expression and p... |
 | pGFPTA - Bacteria Resistance: Ampicillin; Comments:Ito Y., Suzuki M. and Husimi Y, A T-extended vector using a green fluorescent protein as an indicator, Gene 245 (1), 59-63 (2000). |
| Plasmids 60 - 64 | Descriptions |
![small image of pGL4.26[luc2_minP_Hygro]](thumb/pGL4.26[luc2_minP_Hygro]_thu.jpg) | pGL4.26[luc2/minP/Hygro] - Vendor:Promega; Catalog Number: E8441; GenBank Accession Number:DQ904458 |
 | pHT3T7bm+ - Vendor:Boehringer; Comments:Hosts: E.coli. Related vectors: pHT3T7, pHT3T7bm. (Information source: VectorDB.) |
 | pLI50 -
5' Sequencing primer: N/A ;
Bacteria resistance: Ampicillin ;
High or low copy: Don't Know ;
Grow in standard E. coli @ 37C: Yes ;
Selectable markers: Chloramphenicol in S. aureus at 37C ;
Plasmid Provided In: DH5a ;
Principal Investigator: Chia Y. Lee ;
Article: Construction of single-copy integration vectors for Staphylococcus aureus. Lee CY et al. (Gene. 1991 Jul 15. 1... |
 | pMN19 - Gene/insert name: CaMV 35S enhancers ;
Vector backbone: pPZP212 ;
Backbone manufacturer: Hajdukiewicz et al 1994 ;
Cloning site 5': ;
Site destroyed during cloning: No ;
Cloning site 3': See paper and map. ;
Site destroyed during cloning: No ;
5' Sequencing primer: M13 reverse ;
Bacteria resistance: Spectinomycin ;
High or low copy: High Copy ;
Grow in sta... |
 | pT7T3-18 - GenBank Accession Number: L08951; Comments: These data and their annotation were supplied to GenBank by Will Gilbert under the auspices of the GenBank Currator Program. old BRL vector.; Hosts: E.coli.; Related vectors: pBR322, pUC18, PromT7, PromT3, pT7T3-19.; (Information source: VectorDB.) |
| Plasmids 65 - 69 | Descriptions |
 | pT7T3-18U - Vendor: Amersham; Hosts: E.coli NM522.; Related vectors: pUC18, bacteriophage f1, pTZ18U, pT7T3-19U.; (Information source: VectorDB.); Type of vector: Other |
 | pT7T3-19 - GenBank Accession Number: L08952; Comments: These data and their annotation were supplied to GenBank by Will Gilbert under the auspices of the GenBank Currator Program. old BRL vector.; Hosts: E.coli.; Related vectors: pBR322, pUC19, PromT7, PromT3, pT7T3-18.; (Information source: VectorDB.) |
 | pT7T3-19U - Vendor: Amersham; Sequence and Map: Sequence; Hosts: E.coli NM522. Related vectors: pUC18, bacteriophage f1, pTZ19U, pT7T3-18U. (Information source: VectorDB.) |
 | V143 pLP-HA SD - Acceptor - Relevant mutations/deletions: Splice for 5' tags ;
Fusion proteins or tags: HA ;
Terminal: N terminal on backbone;
Vector backbone: NA ;
5' Sequencing primer: CMV-F ;
Bacteria resistance: Kanamycin ;
High or low copy: High Copy ;
Grow in standard E. coli @ 37C: Yes ;
Plasmid Provided In: DH5a ;
Principal Investigator: Tony Pawson ;
Commen... |
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