}jI6}jI6 ID FJ517647; SV 1; circular; other DNA; STD; SYN; 5024 BP. AC FJ517647; DT 18-JAN-2009 (Rel. 99, Created) DT 18-JAN-2009 (Rel. 99, Last updated, Version 1) DE PBR322 based IG-lambda expression vector, complete genome. KW . OS pBR322 based IG-lambda expression vector OC other sequences; artificial sequences; vectors. RN [1] RP 1-5024 RA Wilson P.C.; RT ; RL Submitted (03-DEC-2008) to the EMBL/GenBank/DDBJ databases. RL Medicine/Rheumatology, The University of Chicago, BSLC/JFK Bldg. 924 East RL 57th St., Rm 414, Chicago, IL 60637, USA FH Key Location/Qualifiers FT source 1..5024 FT /organism="pBR322 based IG-lambda expression vector" FT /mol_type="other DNA" FT /db_xref="taxon:588180" FT promoter 1..618 FT /note="CMV promoter" FT misc_feature 929..973 FT /note="antibody signal peptide" FT misc_feature 982..1261 FT /note="human immunoglobulin C-lambda-2 gene" FT misc_feature 1282..1409 FT /note="SV40 PolyA" FT CDS 2987..3847 FT /codon_start=1 FT /transl_table=11 FT /product="beta lactamase" FT /note="Amp resistance" FT /protein_id="ACL79637.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRDDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" SQ Sequence 5024 BP; 1244 A; 1303 C; 1218 G; 1259 T; 0 other; ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca 60 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct 120 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta 180 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac 240 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt 300 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag 360 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat 420 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat 480 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc 540 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt 600 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga 660 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt 720 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta 780 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac 840 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc 900 acctcggttc tatcgattga attccaccat gggatggtca tgtatcatcc tttttctagt 960 agcaactgca accggtgtac actcgagtga ggagcttcaa gccaacaagg ccacactggt 1020 gtgtctcata agtgacttct acccgggagc cgtgacagtg gcctggaagg cagatagcag 1080 ccccgtcaag gcgggagtgg agaccaccac accctccaaa caaagcaaca acaagtacgc 1140 ggccagcagc tatctgagcc tgacgcctga gcagtggaag tcccacagaa gctacagctg 1200 ccaggtcacg catgaaggga gcaccgtgga gaagacagtg gcccctacag aatgttcata 1260 gaagcttggc cgccatggcc caacttgttt attgcagctt ataatggtta caaataaagc 1320 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 1380 tccaaactca tcaatgtatc ttatcatgtc tggatcgatc gggaattaat tcggcgcagc 1440 accatggcct gaaataacct ctgaaagagg aacttggtta ggtaccttct gaggcggaaa 1500 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 1560 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 1620 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 1680 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 1740 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 1800 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tgttaacagc 1860 ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt 1920 aatcgccttg cagcacatcc ccccttcgcc agctggcgta atagcgaaga ggcccgcacc 1980 gatcgccctt cccaacagtt gcgtagcctg aatggcgaat ggcgcctgat gcggtatttt 2040 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc 2100 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 2160 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 2220 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 2280 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc 2340 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 2400 tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga 2460 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 2520 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg 2580 atgccgcata gttaagccaa ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg 2640 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta 2700 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc 2760 gaaacgcgcg aggcagtatt cttgaagacg aaagggcctc gtgatacgcc tatttttata 2820 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt 2880 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 2940 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 3000 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 3060 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 3120 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 3180 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg atgacgccgg 3240 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 3300 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 3360 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 3420 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 3480 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgccag cagcaatggc 3540 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 3600 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 3660 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 3720 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 3780 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 3840 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt 3900 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 3960 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 4020 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 4080 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 4140 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa 4200 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 4260 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 4320 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 4380 caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag 4440 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 4500 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 4560 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 4620 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt 4680 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg 4740 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg 4800 caaaccgcct ctccccgcgc gttggccgat tcattaatcc agctggcacg acaggtttcc 4860 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttacctca ctcattaggc 4920 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata 4980 acaatttcac acaggaaaca gctatgacca tgattacgaa ttaa 5024 !PBR322 based IG-lambda expression"Times New Roman }jI6SacI(9),SpeI(29),NdeI(264) Arialkjk`kVX~V }jI6NcoI(390),SacI(598),EagI(685) ArialV,,~^ }jI6JClaI(914),EcoRI(920),NcoI(928),XhoI(983),XmaI(1043),SmaI(1045),PvuII(1198) Arial;DMM# }jI6PHindIII(1263),NcoI(1274),SfiI(1276),ClaI(1416),NcoI(1443),KpnI(1486),PvuII(1508) ArialD{N}W~WT }jI6,SexAI(1599),NcoI(1739),SfiI(1785),HpaI(1855) Arial!)11m  }jI6PvuII Arial  = }jI6ScaI Arial ` }jI6PvuII Arial/o-f*\PE\}jI6$8CMV_fwd_primer"Times New Roman}jI6PcpCEP_fwd_primer"Times New Roman}jI6Rj LNCX_primer"Times New Roman}jI6C<M Sp6_primer"Times New Roman}jI6C]pEBV_rev_primer"Times New Roman}jI6pBABE_3_primer"Times New Roman}jI6 SV40_promoter"Times New Roman}jI6CɆ SV40_origin"Times New Roman}jI6CSV40pro_F_primer"Times New Roman*}jI6CJZM13_forward20_primer"Times New Roman}jI6CɆSiM13_pUC_fwd_primer"Times New Roman}jI6C@r  f1_origin"Times New Roman}jI6C  pGEX_3_primer"Times New Roman}jI6CɆd  AmpR_promoter"Times New Roman}jI6C  Ampicillin"Times New Roman}jI6C ORF 1"Times New Roman(}jI6CɆ  pBR322_origin"Times New Roman}jI6CɆB_ lac_promoter"Times New Roman}jI6CmM13_pUC_rev_primer"Times New Roman}jI6CM13_reverse_primer"Times New Roman }jI6test"Times New RomanG"BiM# }jI6test"Times New Romanc/-< }jI6test"Times New RomankRjPI@>@>)