Sequence of PBR322 based IG-lambda expression

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Sequence of PBR322 based IG-lambda expression

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Sequence of PBR322 based IG-lambda expression: 5024 BP; 1244 A; 1303 C; 1218 G; 1259 T; 0 other;

SacI(9) .......... .......... .......... .......... ..........

1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca 60

1 aagctcgagc gggctgtaac taataactga tcaataatta tcattagtta atgccccagt 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct 120

61 aatcaagtat cgggtatata cctcaaggcg caatgtattg aatgccattt accgggcgga 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta 180

121 ccgactggcg ggttgctggg ggcgggtaac tgcagttatt actgcataca agggtatcat 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac 240

181 tgcggttatc cctgaaaggt aactgcagtt acccacctca taaatgccat ttgacgggtg 240

.......... .......... .......... .......... .......... ..........

.......... .......... NdeI(264) .......... .......... ..........

241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt 300

241 aaccgtcatg tagttcacat agtatacggt tcatgcgggg gataactgca gttactgcca 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag 360

301 tttaccgggc ggaccgtaat acgggtcatg tactggaata ccctgaaagg atgaaccgtc 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat 420

361 atgtagatgc ataatcagta gcgataatgg taccactacg ccaaaaccgt catgtagtta 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat 480

421 cccgcaccta tcgccaaact gagtgcccct aaaggttcag aggtggggta actgcagtta 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc 540

481 ccctcaaaca aaaccgtggt tttagttgcc ctgaaaggtt ttacagcatt gttgaggcgg 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt 600

541 ggtaactgcg tttacccgcc atccgcacat gccaccctcc agatatattc gtctcgagca 600

........ CMV_fwd_primer ........ .......... ....... pCEP_fwd_primer

.......... .......... .......... .......... .......... ..........

601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga 660

601 aatcacttgg cagtctagcg gacctctgcg gtaggtgcga caaaactgga ggtatcttct 660

pCEP_fwd_primer LNCX_primer .. .......... .......... ..........

.......... .......... EagI(685) .......... .......... ..........

661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt 720

661 gtggccctgg ctaggtcgga ggcgccggcc cttgccacgt aaccttgcgc ctaaggggca 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta 780

721 cggttctcac tgcattcatg gcggatatct cagatatccg ggtgggggaa ccgaagcaat 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac 840

781 cttgcgccga tgttaattat gtattggaat acatagtatg tgtatgctaa atccactgtg 840

.......... .......... .......... .......... .......... Sp6_primer

.......... .......... .......... .......... .......... ..........

841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc 900

841 atatcttatt gtaggtgaaa cggaaagaga ggtgtccaca ggtgagggtc caggttgacg 900

.......... .......... .......... .......... .......... ..........

..... ClaI(914) EcoRI(920) ..... .......... .......... ..........

901 acctcggttc tatcgattga attccaccat gggatggtca tgtatcatcc tttttctagt 960

901 tggagccaag atagctaact taaggtggta ccctaccagt acatagtagg aaaaagatca 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 agcaactgca accggtgtac actcgagtga ggagcttcaa gccaacaagg ccacactggt 1020

961 tcgttgacgt tggccacatg tgagctcact cctcgaagtt cggttgttcc ggtgtgacca 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gtgtctcata agtgacttct acccgggagc cgtgacagtg gcctggaagg cagatagcag 1080

1021 cacagagtat tcactgaaga tgggccctcg gcactgtcac cggaccttcc gtctatcgtc 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ccccgtcaag gcgggagtgg agaccaccac accctccaaa caaagcaaca acaagtacgc 1140

1081 ggggcagttc cgccctcacc tctggtggtg tgggaggttt gtttcgttgt tgttcatgcg 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ......... PvuII(1198)

1141 ggccagcagc tatctgagcc tgacgcctga gcagtggaag tcccacagaa gctacagctg 1200

1141 ccggtcgtcg atagactcgg actgcggact cgtcaccttc agggtgtctt cgatgtcgac 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ccaggtcacg catgaaggga gcaccgtgga gaagacagtg gcccctacag aatgttcata 1260

1201 ggtccagtgc gtacttccct cgtggcacct cttctgtcac cggggatgtc ttacaagtat 1260

.......... .......... .......... .......... .......... ..........

HindIII(1263) ...... .......... .......... .......... ..........

1261 gaagcttggc cgccatggcc caacttgttt attgcagctt ataatggtta caaataaagc 1320

1261 cttcgaaccg gcggtaccgg gttgaacaaa taacgtcgaa tattaccaat gtttatttcg 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 1380

1321 ttatcgtagt gtttaaagtg tttatttcgt aaaaaaagtg acgtaagatc aacaccaaac 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tccaaactca tcaatgtatc ttatcatgtc tggatcgatc gggaattaat tcggcgcagc 1440

1381 aggtttgagt agttacatag aatagtacag acctagctag cccttaatta agccgcgtcg 1440

EBV_rev_primer ...... .......... .......... .......... ..........

NcoI(1443) .......... .......... .......... .......... ..........

1441 accatggcct gaaataacct ctgaaagagg aacttggtta ggtaccttct gaggcggaaa 1500

1441 tggtaccgga ctttattgga gactttctcc ttgaaccaat ccatggaaga ctccgccttt 1500

.......... .......... .......... .......... .......... ..........

PvuII(1508) ........ .......... .......... .......... ..........

1501 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 1560

1501 cttggtcgac accttacaca cagtcaatcc cacacctttc aggggtccga ggggtcgtcc 1560

.......... ........ pBABE_3_primer ...... SV40_promoter ........

.......... .......... .......... .......... .......... ..........

1561 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 1620

1561 gtcttcatac gtttcgtacg tagagttaat cagtcgttgg tccacacctt tcaggggtcc 1620

.......... ........ SV40_promoter ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 1680

1621 gaggggtcgt ccgtcttcat acgtttcgta cgtagagtta atcagtcgtt ggtatcaggg 1680

.......... ........ SV40_promoter ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 1740

1681 cggggattga ggcgggtagg gcggggattg aggcgggtca aggcgggtaa gaggcggggt 1740

.......... ....... SV40_promoter SV40_origin ....... ..........

.......... .......... .......... .......... .......... ..........

1741 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 1800

1741 accgactgat taaaaaaaat aaatacgtct ccggctccgg cggagccgga gactcgataa 1800

SV40_origin SV40_promoter SV40pro_F_primer ........ ..........

.......... .......... .......... .......... .......... HpaI(1855)

1801 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tgttaacagc 1860

1801 ggtcttcatc actcctccga aaaaacctcc ggatccgaaa acgtttttcg acaattgtcg 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt 1920

1861 aaccgtgacc ggcagcaaaa tgttgcagca ctgacccttt tgggaccgca atgggttgaa 1920

. M13_forward20_primer M13_pUC_fwd_primer . .......... ..........

.......... .......... ......... PvuII(1953) ......... ..........

1921 aatcgccttg cagcacatcc ccccttcgcc agctggcgta atagcgaaga ggcccgcacc 1980

1921 ttagcggaac gtcgtgtagg ggggaagcgg tcgaccgcat tatcgcttct ccgggcgtgg 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gatcgccctt cccaacagtt gcgtagcctg aatggcgaat ggcgcctgat gcggtatttt 2040

1981 ctagcgggaa gggttgtcaa cgcatcggac ttaccgctta ccgcggacta cgccataaaa 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc 2100

2041 gaggaatgcg tagacacgcc ataaagtgtg gcgtatgcag tttcgttggt atcatgcgcg 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 2160

2101 ggacatcgcc gcgtaattcg cgccgcccac accaccaatg cgcgtcgcac tggcgatgtg 2160

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 2220

2161 aacggtcgcg ggatcgcggg cgaggaaagc gaaagaaggg aaggaaagag cggtgcaagc 2220

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 2280

2221 ggccgaaagg ggcagttcga gatttagccc ccgagggaaa tcccaaggct aaatcacgaa 2280

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc 2340

2281 atgccgtgga gctggggttt tttgaactaa acccactacc aagtgcatca cccggtagcg 2340

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 2400

2341 ggactatctg ccaaaaagcg ggaaactgca acctcaggtg caagaaatta tcacctgaga 2400

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga 2460

2401 acaaggtttg accttgttgt gagttgggat agagcccgat aagaaaacta aatattccct 2460

f1_origin .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 2520

2461 aaaacggcta aagccggata accaattttt tactcgacta aattgttttt aaattgcgct 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg 2580

2521 taaaattgtt ttataattgc aaatgttaaa ataccacgtg agagtcatgt tagacgagac 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 atgccgcata gttaagccaa ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg 2640

2581 tacggcgtat caattcggtt gaggcgatag cgatgcactg acccagtacc gacgcggggc 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta 2700

2641 tgtgggcggt tgtgggcgac tgcgcgggac tgcccgaaca gacgagggcc gtaggcgaat 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc 2760

2701 gtctgttcga cactggcaga ggccctcgac gtacacagtc tccaaaagtg gcagtagtgg 2760

.......... .......... ........ pGEX_3_primer ........ ..........

.......... .......... .......... .......... .......... ..........

2761 gaaacgcgcg aggcagtatt cttgaagacg aaagggcctc gtgatacgcc tatttttata 2820

2761 ctttgcgcgc tccgtcataa gaacttctgc tttcccggag cactatgcgg ataaaaatat 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt 2880

2821 ccaattacag tactattatt accaaagaat ctgcagtcca ccgtgaaaag cccctttaca 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 2940

2881 cgcgccttgg ggataaacaa ataaaaagat ttatgtaagt ttatacatag gcgagtactc 2940

.......... .......... .......... ........ AmpR_promoter ........

.......... .......... .......... .......... .......... ..........

2941 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 3000

2941 tgttattggg actatttacg aagttattat aactttttcc ttctcatact cataagttgt 3000

.......... .......... .......... .......... ....... Ampicillin ORF 1

.......... .......... .......... .......... .......... ..........

3001 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 3060

3001 aaaggcacag cgggaataag ggaaaaaacg ccgtaaaacg gaaggacaaa aacgagtggg 3060

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 3120

3061 tctttgcgac cactttcatt ttctacgact tctagtcaac ccacgtgctc acccaatgta 3120

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 3180

3121 gcttgaccta gagttgtcgc cattctagga actctcaaaa gcggggcttc ttgcaaaagg 3180

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg atgacgccgg 3240

3181 ttactactcg tgaaaatttc aagacgatac accgcgccat aatagggcac tactgcggcc 3240

.......... ....... Ampicillin ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ScaI(3294)

3241 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 3300

3241 cgttctcgtt gagccagcgg cgtatgtgat aagagtctta ctgaaccaac tcatgagtgg 3300

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 3360

3301 tcagtgtctt ttcgtagaat gcctaccgta ctgtcattct cttaatacgt cacgacggta 3360

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 3420

3361 ttggtactca ctattgtgac gccggttgaa tgaagactgt tgctagcctc ctggcttcct 3420

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 3480

3421 cgattggcga aaaaacgtgt tgtaccccct agtacattga gcggaactag caacccttgg 3480

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgccag cagcaatggc 3540

3481 cctcgactta cttcggtatg gtttgctgct cgcactgtgg tgctacggtc gtcgttaccg 3540

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 3600

3541 ttgttgcaac gcgtttgata attgaccgct tgatgaatga gatcgaaggg ccgttgttaa 3600

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 3660

3601 ttatctgacc tacctccgcc tatttcaacg tcctggtgaa gacgcgagcc gggaaggccg 3660

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 3720

3661 accgaccaaa taacgactat ttagacctcg gccactcgca cccagagcgc catagtaacg 3720

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 3780

3721 tcgtgacccc ggtctaccat tcgggagggc atagcatcaa tagatgtgct gcccctcagt 3780

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 3840

3781 ccgttgatac ctacttgctt tatctgtcta gcgactctat ccacggagtg actaattcgt 3840

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt 3900

3841 aaccattgac agtctggttc aaatgagtat atatgaaatc taactaaatt ttgaagtaaa 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 3960

3901 aattaaattt tcctagatcc acttctagga aaaactatta gagtactggt tttagggaat 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 4020

3961 tgcactcaaa agcaaggtga ctcgcagtct ggggcatctt ttctagtttc ctagaagaac 4020

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

4021 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 4080

4021 tctaggaaaa aaagacgcgc attagacgac gaacgtttgt ttttttggtg gcgatggtcg 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 4140

4081 ccaccaaaca aacggcctag ttctcgatgg ttgagaaaaa ggcttccatt gaccgaagtc 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa 4200

4141 gtctcgcgtc tatggtttat gacaggaaga tcacatcggc atcaatccgg tggtgaagtt 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 4260

4201 cttgagacat cgtggcggat gtatggagcg agacgattag gacaatggtc accgacgacg 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 4320

4261 gtcaccgcta ttcagcacag aatggcccaa cctgagttct gctatcaatg gcctattccg 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 4380

4321 cgtcgccagc ccgacttgcc ccccaagcac gtgtgtcggg tcgaacctcg cttgctggat 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag 4440

4381 gtggcttgac tctatggatg tcgcactcgt aactctttcg cggtgcgaag ggcttccctc 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 4500

4441 tttccgcctg tccataggcc attcgccgtc ccagccttgt cctctcgcgt gctccctcga 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 4560

4501 aggtccccct ttgcggacca tagaaatatc aggacagccc aaagcggtgg agactgaact 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 4620

4561 cgcagctaaa aacactacga gcagtccccc cgcctcggat acctttttgc ggtcgttgcg 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt 4680

4621 ccggaaaaat gccaaggacc ggaaaacgac cggaaaacga gtgtacaaga aaggacgcaa 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg 4740

4681 taggggacta agacacctat tggcataatg gcggaaactc actcgactat ggcgagcggc 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg 4800

4741 gtcggcttgc tggctcgcgt cgctcagtca ctcgctcctt cgccttctcg cgggttatgc 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... PvuII(4843) .........

4801 caaaccgcct ctccccgcgc gttggccgat tcattaatcc agctggcacg acaggtttcc 4860

4801 gtttggcgga gaggggcgcg caaccggcta agtaattagg tcgaccgtgc tgtccaaagg 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttacctca ctcattaggc 4920

4861 gctgaccttt cgcccgtcac tcgcgttgcg ttaattacac tcaatggagt gagtaatccg 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata 4980

4921 tggggtccga aatgtgaaat acgaaggccg agcatacaac acaccttaac actcgcctat 4980

.......... ......... lac_promoter ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 acaatttcac acaggaaaca gctatgacca tgattacgaa ttaa...... .......... 5024

4981 tgttaaagtg tgtcctttgt cgatactggt actaatgctt aatt...... .......... 5024

M13_pUC_rev_primer M13_reverse_primer .... .......... ..........

References

Reference number: [1] Reference positions: 1-5024 Reference author: Wilson P.C.; Reference title: ; Reference location: Submitted (03-DEC-2008) to the EMBL/GenBank/DDBJ databases. Reference location: Medicine/Rheumatology, The University of Chicago, BSLC/JFK Bldg. 924 East Reference location: 57th St., Rm 414, Chicago, IL 60637, USA