Sequence of PGC-1-luciferase

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Sequence of PGC-1-luciferase

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Sequence of PGC-1 alpha promoter 2kb luciferase : 7404 BP; 2050 A; 1548 C; 1801 G; 2005 T; 0 other;

KpnI(5) .......... .......... .......... .......... ..........

1 ggtacccctg tgctctctct agcttcacat accccttttt atgaaggtag aggacagagt 60

1 ccatggggac acgagagaga tcgaagtgta tggggaaaaa tacttccatc tcctgtctca 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggctgtttta agcagatcat tagcttcatg gatgtgctgg gttagtttct tttcttttct 120

61 ccgacaaaat tcgtctagta atcgaagtac ctacacgacc caatcaaaga aaagaaaaga 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tttcttttct ttttttaaag tagaattagg tggcaaaaaa agaaagaaag aaagaaagaa 180

121 aaagaaaaga aaaaaatttc atcttaatcc accgtttttt tctttctttc tttctttctt 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 agaaagaaat tatcttttca aagcaaagaa aaagaaaatc ctgccacaat tcagtgtgag 240

181 tctttcttta atagaaaagt ttcgtttctt tttcttttag gacggtgtta agtcacactc 240

.......... .......... .......... .......... .......... ..........

.......... EcoRV(251) .......... .......... .......... ..........

241 caagttaaga tatcaaaaca ataatgcaga gttatctagt gcaagcaacc atctgtaaga 300

241 gttcaattct atagttttgt tattacgtct caatagatca cgttcgttgg tagacattct 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 agaggtgtgc atgaggtttg aaaaagtatg gtggatgcct aatggatgac aaagagaaac 360

301 tctccacacg tactccaaac tttttcatac cacctacgga ttacctactg tttctctttg 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 caaagtatca gttaccatca ggatgccagg attgcttgat aatgatgaat aaacatcgac 420

361 gtttcatagt caatggtagt cctacggtcc taacgaacta ttactactta tttgtagctg 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tctttatagc taagtggcca gcattatttc acagttgact gtgtggaaag tagagcccat 480

421 agaaatatcg attcaccggt cgtaataaag tgtcaactga cacacctttc atctcgggta 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gacctttgtc ctgaatttta atagtttact gaagttttac attaagtaaa aatctaattg 540

481 ctggaaacag gacttaaaat tatcaaatga cttcaaaatg taattcattt ttagattaac 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gcaagaaaaa ttagtgtttc tttgtgggag tacagagtaa ggctactaat tgcatgtaga 600

541 cgttcttttt aatcacaaag aaacaccctc atgtctcatt ccgatgatta acgtacatct 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 aaatgatccc agggttgtct ctctctctct ctgaattaga aaattaaatg aataatacct 660

601 tttactaggg tcccaacaga gagagagaga gacttaatct tttaatttac ttattatgga 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ttctgatgtt tggaagagga cagttgtagc agtgaagtat attcacctaa tttagaatgt 720

661 aagactacaa accttctcct gtcaacatcg tcacttcata taagtggatt aaatcttaca 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 caaagctgtt ggccatcttg ttctgttcac ttattgacaa actgtcgatt tcattaccat 780

721 gtttcgacaa ccggtagaac aagacaagtg aataactgtt tgacagctaa agtaatggta 780

.......... .......... .......... .......... .......... ..........

.......... SpeI(792) .......... .......... .......... ..........

781 attccctgtc tactagttgt ggacatccaa gcaaacagac cccttatacg gggtctgaag 840

781 taagggacag atgatcaaca cctgtaggtt cgtttgtctg gggaatatgc cccagacttc 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 caaacagcaa gcttgtgggc tttgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg 900

841 gtttgtcgtt cgaacacccg aaacacacac acacacacac acacacacac acacacacac 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tgtgtgtgtg tgcacgctcg ctgcatttct ttctttcact ttactgtatt agtaactgac 960

901 acacacacac acgtgcgagc gacgtaaaga aagaaagtga aatgacataa tcattgactg 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tatataaagt cagactgaga gaagtcacca atgttttcct tctaagggga gagaaaataa 1020

961 atatatttca gtctgactct cttcagtggt tacaaaagga agattcccct ctcttttatt 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 agagcacatt aaattaacct cagtggaata ggagtttaaa tgaatggtgc tttataaatt 1080

1021 tctcgtgtaa tttaattgga gtcaccttat cctcaaattt acttaccacg aaatatttaa 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 atatttagat gcatagggac ttttttcttc ttctaaatta ctgtcaagta aaggaaatga 1140

1081 tataaatcta cgtatccctg aaaaaagaag aagatttaat gacagttcat ttcctttact 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gctggttttt gagtctgtgg ggtttgaggt attgtgaaaa tatgttttca aaggtcccct 1200

1141 cgaccaaaaa ctcagacacc ccaaactcca taacactttt atacaaaagt ttccagggga 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gtgcatttct cactgggcct ggaagggtta agtctgagca cccaagtgtt atggaaagtg 1260

1201 cacgtaaaga gtgacccgga ccttcccaat tcagactcgt gggttcacaa tacctttcac 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ctgagagttg gttatgtcct ctgtctgtaa tgtcacagga aaaacagtgg cacctgcatt 1320

1261 gactctcaac caatacagga gacagacatt acagtgtcct ttttgtcacc gtggacgtaa 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 acccctcatt gactcaggaa cgacaaaaaa gtattagtaa gcaaagctca agaaatgagt 1380

1321 tggggagtaa ctgagtcctt gctgtttttt cataatcatt cgtttcgagt tctttactca 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 atctctgctg ataccatttc agtgtttttc cttcattccc tggacattct tgatttcaaa 1440

1381 tagagacgac tatggtaaag tcacaaaaag gaagtaaggg acctgtaaga actaaagttt 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aacaaactgt acagcccaag gcactagggt tggagtccaa tgtttattca aaaaggcacc 1500

1441 ttgtttgaca tgtcgggttc cgtgatccca acctcaggtt acaaataagt ttttccgtgg 1500

.......... .......... .......... .......... .......... ..........

.......... .......... NdeI(1528) .......... .......... ..........

1501 ctgaagccat gaggaagact gtgctacata tgagaaaaga aataaggggt gggggcaggt 1560

1501 gacttcggta ctccttctga cacgatgtat actcttttct ttattcccca cccccgtcca 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gagtagctaa gctgtttcag ggatggcagc agcaattgta ttttctagca tttgttttct 1620

1561 ctcatcgatt cgacaaagtc cctaccgtcg tcgttaacat aaaagatcgt aaacaaaaga 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gggagcctat gagatccacg gaaagaatca tgagggggaa cccaagagtc tagggtgttg 1680

1621 ccctcggata ctctaggtgc ctttcttagt actccccctt gggttctcag atcccacaac 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tggcttgctt gctttacaag gagcaaggca aactgcagta acagtttagg agactgcatt 1740

1681 accgaacgaa cgaaatgttc ctcgttccgt ttgacgtcat tgtcaaatcc tctgacgtaa 1740

.......... .......... .......... .......... .......... ..........

......... PvuII(1760) ......... .......... .......... ..........

1741 ctctactgcc aaggagacag ctgatttggg gtagagaaat ttgtttagac ctaaacaaat 1800

1741 gagatgacgg ttcctctgtc gactaaaccc catctcttta aacaaatctg gatttgttta 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gtggcggttt tgttgactaa acatggaaag aaagaaagaa agaaagaaag aaagaaagaa 1860

1801 caccgccaaa acaactgatt tgtacctttc tttctttctt tctttctttc tttctttctt 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 agaaagaaag aaagaaagaa agaaacaaag aaagaaagaa agaaaggaag gaaggaagga 1920

1861 tctttctttc tttctttctt tctttgtttc tttctttctt tctttccttc cttccttcct 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 agaaaggaag aaaggaagga aggaaggaag gaaagaaagg agagagagaa agaaaatcgg 1980

1921 tctttccttc tttccttcct tccttccttc ctttctttcc tctctctctt tcttttagcc 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gggtgttgcc ttcaaacact cctctaatag ggagggaaaa aaaagaatct catgaaaatg 2040

1981 cccacaacgg aagtttgtga ggagattatc cctccctttt ttttcttaga gtacttttac 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tatcacatga ggagcgcttg cttcagttcc aagctgagtc tggggctact tggaaaccat 2100

2041 atagtgtact cctcgcgaac gaagtcaagg ttcgactcag accccgatga acctttggta 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ttcttaaagc acacacattt taggcaaggg tgtagttact gtgtcagtaa caggggatct 2160

2101 aagaatttcg tgtgtgtaaa atccgttccc acatcaatga cacagtcatt gtcccctaga 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ttgctatttg cctgttttgg atggaaaata aatttaaaaa aaaaagattg caggagattt 2220

2161 aacgataaac ggacaaaacc taccttttat ttaaattttt tttttctaac gtcctctaaa 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gagttattat gtgagcaggg ctccggttta gagttggtgg cattcaaagc tggcttcagt 2280

2221 ctcaataata cactcgtccc gaggccaaat ctcaaccacc gtaagtttcg accgaagtca 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cacagtgtga tgcttgaagc ctcccaaagg ccaagtgttt ccttttcttt cttctatttt 2340

2281 gtgtcacact acgaacttcg gagggtttcc ggttcacaaa ggaaaagaaa gaagataaaa 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ......... AatII(2400)

2341 ttttttcctc tctctctaag cgttacttca ctgaggcaga gggctgcctt ggagtgacgt 2400

2341 aaaaaaggag agagagattc gcaatgaagt gactccgtct cccgacggaa cctcactgca 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 caggagtttg tgcagcaagc ttgcacagga gaagggaggc tgggtgagtg acagcccagc 2460

2401 gtcctcaaac acgtcgttcg aacgtgtcct cttccctccg acccactcac tgtcgggtcg 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ctacttttta atagctttgt catgtgactg gggactgtag taagacaggt gccttcagtt 2520

2461 gatgaaaaat tatcgaaaca gtacactgac ccctgacatc attctgtcca cggaagtcaa 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cactctcagt aaggggctgg ttgcctgcat gagtgtgtgc tgtgtgtcag agtggattgg 2580

2521 gtgagagtca ttccccgacc aacggacgta ctcacacacg acacacagtc tcacctaacc 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 agttgaaaaa gcttgactgg cgtcattcgg gagctggctc gagatctgcg atctaagtaa 2640

2581 tcaacttttt cgaactgacc gcagtaagcc ctcgaccgag ctctagacgc tagattcatt 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... NcoI(2672) .......... ..........

2641 gcttggcatt ccggtactgt tggtaaagcc accatggaag acgccaaaaa cataaagaaa 2700

2641 cgaaccgtaa ggccatgaca accatttcgg tggtaccttc tgcggttttt gtatttcttt 2700

.......... .......... .......... ... ORF 1 firefly_Luciferase ...

NarI(2707) .......... .......... .......... .......... ..........

2701 ggcccggcgc cattctatcc gctggaagat ggaaccgctg gagagcaact gcataaggct 2760

2701 ccgggccgcg gtaagatagg cgaccttcta ccttggcgac ctctcgttga cgtattccga 2760

.......... ... ORF 1 firefly_Luciferase .. LucNrev_pri .........

.......... .......... .......... .......... .......... ..........

2761 atgaagagat acgccctggt tcctggaaca attgctttta cagatgcaca tatcgaggtg 2820

2761 tacttctcta tgcgggacca aggaccttgt taacgaaaat gtctacgtgt atagctccac 2820

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... ......... BstBI(2843) ......... .......... ..........

2821 gacatcactt acgctgagta cttcgaaatg tccgttcggt tggcagaagc tatgaaacga 2880

2821 ctgtagtgaa tgcgactcat gaagctttac aggcaagcca accgtcttcg atactttgct 2880

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tatgggctga atacaaatca cagaatcgtc gtatgcagtg aaaactctct tcaattcttt 2940

2881 atacccgact tatgtttagt gtcttagcag catacgtcac ttttgagaga agttaagaaa 2940

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 atgccggtgt tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa cgacatttat 3000

2941 tacggccaca acccgcgcaa taaatagcct caacgtcaac gcgggcgctt gctgtaaata 3000

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 aatgaacgtg aattgctcaa cagtatgggc atttcgcagc ctaccgtggt gttcgtttcc 3060

3001 ttacttgcac ttaacgagtt gtcatacccg taaagcgtcg gatggcacca caagcaaagg 3060

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaagc tcccaatcat ccaaaaaatt 3120

3061 tttttcccca acgtttttta aaacttgcac gtttttttcg agggttagta ggttttttaa 3120

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 attatcatgg attctaaaac ggattaccag ggatttcagt cgatgtacac gttcgtcaca 3180

3121 taatagtacc taagattttg cctaatggtc cctaaagtca gctacatgtg caagcagtgt 3180

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tctcatctac ctcccggttt taatgaatac gattttgtgc cagagtcctt cgatagggac 3240

3181 agagtagatg gagggccaaa attacttatg ctaaaacacg gtctcaggaa gctatccctg 3240

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... BclI(3254) .......... .......... .......... ..........

3241 aagacaattg cactgatcat gaactcctct ggatctactg gtctgcctaa aggtgtcgct 3300

3241 ttctgttaac gtgactagta cttgaggaga cctagatgac cagacggatt tccacagcga 3300

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ctgcctcata gaactgcctg cgtgagattc tcgcatgcca gagatcctat ttttggcaat 3360

3301 gacggagtat cttgacggac gcactctaag agcgtacggt ctctaggata aaaaccgtta 3360

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 caaatcattc cggatactgc gattttaagt gttgttccat tccatcacgg ttttggaatg 3420

3361 gtttagtaag gcctatgacg ctaaaattca caacaaggta aggtagtgcc aaaaccttac 3420

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tttactacac tcggatattt gatatgtgga tttcgagtcg tcttaatgta tagatttgaa 3480

3421 aaatgatgtg agcctataaa ctatacacct aaagctcagc agaattacat atctaaactt 3480

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gaagagctgt ttctgaggag ccttcaggat tacaagattc aaagtgcgct gctggtgcca 3540

3481 cttctcgaca aagactcctc ggaagtccta atgttctaag tttcacgcga cgaccacggt 3540

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 accctattct ccttcttcgc caaaagcact ctgattgaca aatacgattt atctaattta 3600

3541 tgggataaga ggaagaagcg gttttcgtga gactaactgt ttatgctaaa tagattaaat 3600

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cacgaaattg cttctggtgg cgctcccctc tctaaggaag tcggggaagc ggttgccaag 3660

3601 gtgctttaac gaagaccacc gcgaggggag agattccttc agccccttcg ccaacggttc 3660

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 aggttccatc tgccaggtat caggcaagga tatgggctca ctgagactac atcagctatt 3720

3661 tccaaggtag acggtccata gtccgttcct atacccgagt gactctgatg tagtcgataa 3720

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ctgattacac ccgaggggga tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt 3780

3721 gactaatgtg ggctccccct actatttggc ccgcgccagc catttcaaca aggtaaaaaa 3780

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gaagcgaagg ttgtggatct ggataccggg aaaacgctgg gcgttaatca aagaggcgaa 3840

3781 cttcgcttcc aacacctaga cctatggccc ttttgcgacc cgcaattagt ttctccgctt 3840

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ctgtgtgtga gaggtcctat gattatgtcc ggttatgtaa acaatccgga agcgaccaac 3900

3841 gacacacact ctccaggata ctaatacagg ccaatacatt tgttaggcct tcgctggttg 3900

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gccttgattg acaaggatgg atggctacat tctggagaca tagcttactg ggacgaagac 3960

3901 cggaactaac tgttcctacc taccgatgta agacctctgt atcgaatgac cctgcttctg 3960

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gaacacttct tcatcgttga ccgcctgaag tctctgatta agtacaaagg ctatcaggtg 4020

3961 cttgtgaaga agtagcaact ggcggacttc agagactaat tcatgtttcc gatagtccac 4020

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gctcccgctg aattggaatc catcttgctc caacacccca acatcttcga cgcaggtgtc 4080

4021 cgagggcgac ttaaccttag gtagaacgag gttgtggggt tgtagaagct gcgtccacag 4080

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg ccgttgttgt tttggagcac 4140

4081 cgtccagaag ggctgctact gcggccactt gaagggcggc ggcaacaaca aaacctcgtg 4140

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ggaaagacga tgacggaaaa agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg 4200

4141 cctttctgct actgcctttt tctctagcac ctaatgcagc ggtcagttca ttgttggcgc 4200

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa 4260

4201 tttttcaacg cgcctcctca acacaaacac ctgcttcatg gctttccaga atggcctttt 4260

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca agaagggcgg aaagatcgcc 4320

4261 gagctgcgtt ctttttagtc tctctaggag tatttccggt tcttcccgcc tttctagcgg 4320

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

XbaI(4328) .......... .......... .......... .......... ..........

4321 gtgtaattct agagtcgggg cggccggccg cttcgagcag acatgataag atacattgat 4380

4321 cacattaaga tctcagcccc gccggccggc gaagctcgtc tgtactattc tatgtaacta 4380

.......... .......... .......... .......... ......... SV40_PA_term

.......... .......... .......... .......... .......... ..........

4381 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt 4440

4381 ctcaaacctg tttggtgttg atcttacgtc acttttttta cgaaataaac actttaaaca 4440

EBV_rev_pri ....... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... HpaI(4488) ..........

4441 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat 4500

4441 ctacgataac gaaataaaca ttggtaatat tcgacgttat ttgttcaatt gttgttgtta 4500

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa 4560

4501 acgtaagtaa aatacaaagt ccaagtcccc ctccacaccc tccaaaaaat ttcgttcatt 4560

.......... .......... .......... .......... .......... ..........

.......... ......... BamHI(4590) SalI(4596) ......... ..........

4561 aacctctaca aatgtggtaa aatcgataag gatccgtcga ccgatgccct tgagagcctt 4620

4561 ttggagatgt ttacaccatt ttagctattc ctaggcagct ggctacggga actctcggaa 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac 4680

4621 gttgggtcag tcgaggaagg ccacccgcgc cccgtactga tagcagcggc gtgaatactg 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc 4740

4681 acagaagaaa tagtacgttg agcatcctgt ccacggccgt cgcgagaagg cgaaggagcg 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 4800

4741 agtgactgag cgacgcgagc cagcaagccg acgccgctcg ccatagtcga gtgagtttcc 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 4860

4801 gccattatgc caataggtgt cttagtcccc tattgcgtcc tttcttgtac actcgttttc 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 4920

4861 cggtcgtttt ccggtccttg gcatttttcc ggcgcaacga ccgcaaaaag gtatccgagg 4920

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

4921 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 4980

4921 cggggggact gctcgtagtg tttttagctg cgagttcagt ctccaccgct ttgggctgtc 4980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 5040

4981 ctgatatttc tatggtccgc aaagggggac cttcgaggga gcacgcgaga ggacaaggct 5040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 5100

5041 gggacggcga atggcctatg gacaggcgga aagagggaag cccttcgcac cgcgaaagag 5100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5101 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 5160

5101 ttacgagtgc gacatccata gagtcaagcc acatccagca agcgaggttc gacccgacac 5160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5161 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 5220

5161 acgtgcttgg ggggcaagtc gggctggcga cgcggaatag gccattgata gcagaactca 5220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 5280

5221 ggttgggcca ttctgtgctg aatagcggtg accgtcgtcg gtgaccattg tcctaatcgt 5280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 5340

5281 ctcgctccat acatccgcca cgatgtctca agaacttcac caccggattg atgccgatgt 5340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5341 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 5400

5341 gatcttcctg tcataaacca tagacgcgag acgacttcgg tcaatggaag cctttttctc 5400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 5460

5401 aaccatcgag aactaggccg tttgtttggt ggcgaccatc gccaccaaaa aaacaaacgt 5460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5461 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 5520

5461 tcgtcgtcta atgcgcgtct ttttttccta gagttcttct aggaaactag aaaagatgcc 5520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5521 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 5580

5521 ccagactgcg agtcaccttg cttttgagtg caattcccta aaaccagtac tctaatagtt 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 5640

5581 tttcctagaa gtggatctag gaaaatttaa tttttacttc aaaatttagt tagatttcat 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 5700

5641 atatactcat ttgaaccaga ctgtcaatgg ttacgaatta gtcactccgt ggatagagtc 5700

.......... .......... .......... ....... ORF 2 Ampicillin .......

.......... .......... .......... .......... .......... ..........

5701 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 5760

5701 gctagacaga taaagcaagt aggtatcaac ggactgaggg gcagcacatc tattgatgct 5760

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 5820

5761 atgccctccc gaatggtaga ccggggtcac gacgttacta tggcgctctg ggtgcgagtg 5820

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 5880

5821 gccgaggtct aaatagtcgt tatttggtcg gtcggccttc ccggctcgcg tcttcaccag 5880

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... AseI(5911) .......... ..........

5881 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 5940

5881 gacgttgaaa taggcggagg taggtcagat aattaacaac ggcccttcga tctcattcat 5940

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 6000

5941 caagcggtca attatcaaac gcgttgcaac aacggtaacg atgtccgtag caccacagtg 6000

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 6060

6001 cgagcagcaa accataccga agtaagtcga ggccaagggt tgctagttcc gctcaatgta 6060

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 6120

6061 ctagggggta caacacgttt tttcgccaat cgaggaagcc aggaggctag caacagtctt 6120

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 6180

6121 cattcaaccg gcgtcacaat agtgagtacc aataccgtcg tgacgtatta agagaatgac 6180

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 6240

6181 agtacggtag gcattctacg aaaagacact gaccactcat gagttggttc agtaagactc 6240

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 6300

6241 ttatcacata cgccgctggc tcaacgagaa cgggccgcag ttatgcccta ttatggcgcg 6300

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 6360

6301 gtgtatcgtc ttgaaatttt cacgagtagt aaccttttgc aagaagcccc gcttttgaga 6360

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 6420

6361 gttcctagaa tggcgacaac tctaggtcaa gctacattgg gtgagcacgt gggttgacta 6420

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 6480

6421 gaagtcgtag aaaatgaaag tggtcgcaaa gacccactcg tttttgtcct tccgttttac 6480

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 6540

6481 ggcgtttttt cccttattcc cgctgtgcct ttacaactta tgagtatgag aaggaaaaag 6540

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 6600

6541 ttataataac ttcgtaaata gtcccaataa cagagtactc gcctatgtat aaacttacat 6600

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

6601 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 6660

6601 aaatcttttt atttgtttat ccccaaggcg cgtgtaaagg ggcttttcac ggtggactgc 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 6720

6661 gcgggacatc gccgcgtaat tcgcgccgcc cacaccacca atgcgcgtcg cactggcgat 6720

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

6721 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 6780

6721 gtgaacggtc gcgggatcgc gggcgaggaa agcgaaagaa gggaaggaaa gagcggtgca 6780

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg 6840

6781 agcggccgaa aggggcagtt cgagatttag cccccgaggg aaatcccaag gctaaatcac 6840

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 6900

6841 gaaatgccgt ggagctgggg ttttttgaac taatcccact accaagtgca tcacccggta 6900

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 6960

6901 gcgggactat ctgccaaaaa gcgggaaact gcaacctcag gtgcaagaaa ttatcacctg 6960

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 7020

6961 agaacaaggt ttgaccttgt tgtgagttgg gatagagcca gataagaaaa ctaaatattc 7020

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 7080

7021 cctaaaacgg ctaaagccgg ataaccaatt ttttactcga ctaaattgtt tttaaattgc 7080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 cgaattttaa caaaatatta acgtttacaa tttcccattc gccattcagg ctgcgcaact 7140

7081 gcttaaaatt gttttataat tgcaaatgtt aaagggtaag cggtaagtcc gacgcgttga 7140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagcccaag ctaccatgat 7200

7141 caacccttcc cgctagccac gcccggagaa gcgataatgc ggtcgggttc gatggtacta 7200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... NotI(7237) .......... ..........

7201 aagtaagtaa tattaaggta cgggaggtac ttggagcggc cgcaataaaa tatctttatt 7260

7201 ttcattcatt ataattccat gccctccatg aacctcgccg gcgttatttt atagaaataa 7260

.......... .......... .......... .......... . synth_PA_term TB up_PA_term

.......... .......... .......... .......... .......... ..........

7261 ttcattacat ctgtgtgttg gttttttgtg tgaatcgata gtactaacat acgctctcca 7320

7261 aagtaatgta gacacacaac caaaaaacac acttagctat catgattgta tgcgagaggt 7320

.......... . synth_PA_term TB up_PA_term . .......... ..........

.......... .......... .......... .......... .......... ..........

7321 tcaaaacaaa acgaaacaaa acaaactagc aa aataggct gtccccagtg caagtgcagg 7380

7321 agttttgttt tgctttgttt tgtttgatcg ttttatccga caggggtcac gttcacgtcc 7380

...... synth_PA_term TB up_PA_term RVpri3_pri ...... ..........

.......... .......... .......... .......... .......... ..........

7381 tgccagaaca tttctctatc gata...... .......... .......... .......... 7404

7381 acggtcttgt aaagagatag ctat...... .......... .......... .......... 7404

TB up_PA_term ...... .......... .......... .......... ..........