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    Plasmid map of RCASBP-Y DV

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    Gene/insert name: Gateway ccdB reading frame cassette
    Insert size (bp): 2200
    Vector backbone: RCASBP-Y
    Type of vector: Retroviral
    Backbone size (bp): 11600
    Cloning site 5': PmeI
    Site destroyed during cloning: Yes
    Cloning site 3': PmeI
    Site destroyed during cloning: Yes
    5' Sequencing primer: gagctgagctgactctgctggtgg c
    3' Sequencing primer: cagatacgcgtatatctggc
    Bacteria resistance: Ampicillin,Chloramphenicol
    High or low copy: Low Copy
    Grow in standard E. coli @ 37C: No
    Please specify bacterial strain for growth and growth condition: Db3.1 cells due to presence of ccdB gene Plasmid Provided In: DB3.1
    Principal Investigator: William J. Pavan
    Article: Generation of RCAS vectors useful for functional genomic analyses. Loftus SK et al. (DNA Res. 2001 Oct 31. 8(5):221-6. Pubmed)

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