k"k"8XLOCUS RCASBP-Y DV 13373 bp DNA SYN 31-Aug-2007 DEFINITION RCASBP-Y DV ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..13373 /organism="RCASBP-Y DV" /mol_type="other DNA" CDS 372..2483 /label="ORF frame 3" /translation="MEAVIKVISSACKTYCGKISPSKKEIGAMLSLLQKEGLLMSPSD LYSPGSWDPITAALSQRAMVLGKSGELKTWGLVLGALKAAREEQVTSEQAKFWLGLGG GRVSPPGPECIEKPATERRIDKGEEVGETTVQRDAKMAPEEAATPKTVGTSCYHCGTA VGCNCATATASAPPPPYVGSGLYPSLAGVGEQQGQGDNTSRGAEQPREEPGHAGQAPG PALTDWARVREELASTGPPVVAMPVVIKTEGPAWTPLEPKLITRLADTVRTKGLRSPI TMAEVEALMSSPLLPHDVTNLMRVILGPAPYALWMDAWGVQLQTVIAAATRDPRHPAN GQGRGERTNLDRLKGLADGMVGNPQGQAALLRPGELVAITASALQAFREVARLAEPAG PWADITQGPSESFVDFANRLIKAVEGSDLPPSARAPVIIDCFRQKSQPDIQQLIRAAP STLTTPGEIIKYVLDRQKIAPLTDQGIAAAMSSAIQPLVMAVVNRERDGQTGSGGRAR GLCYTCGSPGHYQAQCPKKRKSGNSRERCQLCDGMGHNAKQCRKRDGNQGQRPGRGLS SGPWPGPEQPAVSLAMTMEHKDRPLVRVILTNTGSHPVKQRSVYITALLDSGADITII SEEDWPTDWPVVDTANPQIHGIGGGIPMRKSRDMIEVGVINRDGSLERPLLLFPAVAM VRGSILGRDCLQGLGLRLTNL*" CDS complement(3329..4120) /label="ORF frame 2" /translation="MADLWLNASSKIKAAAVDGTPSCPILSNIFATNAESVTTLVGVV GHSRSAMATARASSCCTLAPKSTISFISHLGPSLQTTTPLWVLEEASVKTVGPGTGWS VTLTFRETCSGRAMSKIDGVSLLLIFPANPLSANRIPSGSGRSSRKHAGNRRISTSLP KVRTAEARSLVIKRVSTSNQAVNALVGWVENNHKHGLGCVDSPCPRTPIAPCSHLATA PSRGWAGSHRSKAAVVLSCTISLQAIFMSRFHSLASLGSDPRNCS*" CDS 2811..5189 /label="ORF frame 3" /translation="MVLDLKDCFFSIPLAEQDREAFAFTLPSVNNQAPARRFQWKVLP QGMTCSPTICQLVVGQVLEPLRLKHPALRMLHYMDDLLLAASSHDGLEAAGKEVIGTL ERAGFTISPDKIQREPGVQYLGYKLGSTYVAPVGLVAEPRIATLWDVQKLVGSLQWLR PALGIPPRLMGPFYEQLRGSDPNEAREWNLDMKMAWREIVQLSTTAALERWDPAQPLE GAVARCEQGAIGVLGQGLSTHPRPCLWLFSTQPTKAFTAWLEVLTLLITKLRASAVRT FGKEVDILLLPACFREDLPLPEGILLALRGFAGKIRSSDTPSIFDIARPLHVSLKVRV TDHPVPGPTVFTDASSSTHKGVVVWREGPRWEIKEIVDLGASVQQLEARAVAMALLLW PTTPTNVVTDSAFVAKMLLKMGQEGVPSTAAAFILEDALSQRSAMAAVLHVRSHSEVP GFFTEGNDVADSQATFQAYPLREAKDLHTALHIGPRALSKACNISMQQAREVVQTCPH CNSAPALEAGVNPRGLGPLQIWQTDFTLEPRMAPRSWLAVTVDTASSAIVVTQHGRVT SVAAQHHWATAIAVLGRPKAIKTDNGSCFTSRSTREWLARWGIAHTTGIPGNSQGQAM VERANRLLKDKIRVLAEGDGFMKRIPASKQGELLAKAMYALNHFERGENTKTPVQKHW RPTVLTEGPPVKIRIETGEWEKGWNVLVWGRGYAAVKNRDTDKVIWVPSRKVKPDVTQ KDEVTKKDEASPLFAGISDWIPWEDEQEGLQGETASNKQERPGEDTLAANES*" CDS 5053..6882 /label="ORF frame 1" /translation="MRRALFLQAFLTGYPGKTSKKDSKEKPLATSKKDPEKTPLLPTR VNYILIIGVLVLCEVTGVRADVHLLEQPGNLWITWANRTGQTDFCLSTQSATSPFQTC LIGIPSPISEGDFKGYVSDTNCTTLGTDRLVSSADFTGGPDNSTTLTYRKVSCLLLKL NVSMWDEPPELQLLGSQSLPNITNIAQISGITGGCVGFRPQGVPWYLGWSRQEATRFL LRHPSFSKSTEPFTVVTADRHNLFMGSEYCGAYGYRFWNMYNCSQVGRQYRCGNARTP RTGLPEIQCTRRGGKWVNQSQEINESEPFSFTVNCTASSLGNASGCCGKAGTILPGKW VDSTQGSFTKPKALPPAIFLICGDRAWQGIPSRPVGGPCYLGKLTMLAPKHTDILKVL VNSSRTGIRRKRSTSHLDDTCSDEVQLWGPTARIFASILAPGVAAAQALREIERLACW SVKQANLTTSLLGDLLDDVTSIRHAVLQNRAAIDFLLLAHGHGCEDVAGMCCFNLSDQ SESIQKKFQLMKEHVNKIGVDSDLIGSWLRGLFGGIGEWAVHLLKGLLLGLVVILLLV VCLPCLLQMLCGNRRKMINNSISYHTEYKKLQKACGQPESRIV*" misc_feature 7048..7172 /label="attR1" misc_feature complement(7072..7172) /label="attR2" promoter 7197..7220 /label="lac_promoter" gene 7281..7938 /label="CAT/CamR(variant)" /gene="CAT/CamR(variant)" misc_feature 8315..8584 /label="ccdB" misc_feature 8625..8725 /label="attR2" misc_feature complement(8625..8732) /label="attR1" misc_feature 9124..9142 /label="pREP_fwd_primer" gene 9399..9689 /label="tet(900-1191)" /gene="tet(900-1191)" misc_feature complement(9763..9785) /label="M13_pUC_rev_primer" promoter 9992..10020 /label="AmpR_promoter" CDS 10062..10922 /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene 10062..10922 /label="Ampicillin" /gene="Ampicillin" rep_origin 11077..11696 /label="pBR322_origin" misc_feature 12093..12115 /label="pGEX_3_primer" misc_feature complement(12109..12300) /label="ROP" misc_feature 13358..3 /label="pREP_fwd_primer" ORIGIN 1 GCCATTTGAC CATTCACCAC ATTGGTGTGC ACCTGGGTTG ATGGCCGGAC CGTTGATTCC 61 CTGACGACTA CGAGCACCTG CATGAAGCAG AAGGCTTCAT TTGGTGACCC CGACGTGATA 121 GTTAGGGAAT AGTGGTCGGC CACAGACGGC GTGGCGATCC TGTCTCCATC CGTCTCGTCT 181 ATCGGGAGGC GAGTTCGATG ACCCTGGTGG AGGGGGCTGC GGCTTAGGGA GGCAGAAGCT 241 GAGTACCGTC GGAGGGAGCT CCAGGGCCCG GAGCGACTGA CCCCTGCCGA GAACTCAGAG 301 GGTCGTCGGA AGACGGAGAG TGAGCCCGAC GACCACCCCA GGCACGTCTT TGGTCGGCCT 361 GCGGATCAAG CATGGAAGCC GTCATTAAGG TGATTTCGTC CGCGTGTAAA ACCTATTGCG 421 GGAAAATCTC TCCTTCTAAG AAGGAAATAG GGGCCATGTT GTCCCTGTTA CAAAAGGAAG 481 GGTTGCTTAT GTCTCCCTCA GATTTATATT CTCCGGGGTC CTGGGATCCC ATCACTGCGG 541 CGCTCTCCCA GCGGGCAATG GTACTTGGAA AATCGGGAGA GTTAAAAACC TGGGGATTGG 601 TTTTGGGGGC ATTGAAGGCG GCTCGAGAGG AACAGGTTAC ATCTGAGCAA GCAAAGTTTT 661 GGTTGGGATT AGGGGGAGGG AGGGTCTCTC CCCCAGGTCC GGAGTGCATC GAGAAACCAG 721 CTACGGAGCG GCGAATCGAC AAAGGGGAGG AGGTGGGAGA AACAACTGTG CAGCGAGATG 781 CGAAGATGGC GCCAGAGGAA GCGGCCACAC CTAAAACCGT TGGCACATCC TGCTATCATT 841 GCGGAACAGC TGTTGGCTGC AATTGCGCCA CCGCCACAGC CTCGGCCCCT CCTCCCCCTT 901 ATGTGGGGAG TGGTTTGTAT CCTTCCCTGG CGGGGGTGGG AGAGCAGCAG GGCCAGGGAG 961 ATAACACGTC TCGGGGGGCG GAGCAGCCAA GGGAGGAGCC AGGGCACGCG GGTCAGGCCC 1021 CTGGGCCGGC CCTGACTGAC TGGGCAAGGG TAAGGGAGGA GCTTGCGAGT ACTGGTCCGC 1081 CCGTGGTGGC CATGCCTGTA GTGATTAAGA CAGAGGGACC CGCCTGGACC CCTCTGGAGC 1141 CAAAATTGAT CACAAGACTG GCTGATACGG TCAGGACCAA GGGCTTACGA TCCCCGATCA 1201 CTATGGCAGA AGTGGAAGCG CTCATGTCCT CCCCGTTGCT GCCGCATGAC GTCACGAATC 1261 TAATGAGAGT GATTTTAGGA CCTGCCCCAT ATGCCTTATG GATGGACGCT TGGGGAGTCC 1321 AACTCCAGAC GGTTATAGCG GCAGCCACTC GCGACCCCCG ACACCCAGCG AACGGTCAAG 1381 GGCGGGGGGA ACGGACTAAC TTGGATCGAT TAAAGGGCTT AGCTGATGGG ATGGTGGGCA 1441 ACCCACAGGG TCAGGCCGCA TTATTAAGAC CGGGGGAATT GGTTGCTATT ACGGCGTCGG 1501 CTCTCCAGGC GTTTAGAGAA GTTGCCCGGC TGGCGGAACC TGCAGGTCCA TGGGCGGACA 1561 TCACGCAGGG ACCATCTGAG TCCTTTGTTG ATTTTGCCAA TCGGCTTATA AAGGCGGTTG 1621 AGGGGTCAGA TCTCCCGCCT TCCGCGCGGG CTCCGGTGAT CATTGACTGC TTTAGGCAGA 1681 AGTCACAGCC AGATATTCAG CAGCTTATAC GGGCAGCACC CTCCACGCTG ACCACCCCAG 1741 GAGAGATAAT CAAATATGTG CTAGACAGGC AGAAGATTGC CCCTCTTACG GATCAAGGCA 1801 TAGCCGCGGC CATGTCGTCT GCTATCCAGC CCTTAGTTAT GGCAGTAGTC AATAGAGAGA 1861 GGGATGGACA AACTGGGTCG GGTGGTCGTG CCCGAGGGCT CTGCTACACT TGTGGATCCC 1921 CGGGACATTA TCAGGCACAG TGCCCGAAAA AACGAAAGTC AGGAAACAGC CGTGAGCGAT 1981 GTCAGCTGTG TGACGGGATG GGACACAACG CTAAACAGTG TAGGAAGCGG GATGGCAACC 2041 AGGGCCAACG CCCAGGAAGA GGTCTCTCTT CGGGGCCGTG GCCCGGCCCT GAGCAGCCTG 2101 CCGTCTCGTT AGCGATGACA ATGGAACATA AAGATCGCCC CTTGGTTAGG GTCATTCTGA 2161 CTAACACTGG GAGTCATCCA GTCAAACAAC GTTCGGTGTA TATCACCGCG CTGTTGGACT 2221 CCGGAGCGGA CATCACTATT ATTTCGGAGG AGGATTGGCC TACTGATTGG CCGGTGGTGG 2281 ACACCGCGAA CCCACAGATC CATGGCATAG GAGGGGGAAT TCCCATGCGA AAATCCCGGG 2341 ATATGATAGA GGTGGGGGTT ATTAACCGAG ACGGGTCGTT GGAGCGACCC CTGCTCCTCT 2401 TCCCCGCAGT CGCTATGGTT AGAGGGAGTA TCCTAGGAAG AGATTGTCTG CAGGGCCTAG 2461 GGCTCCGCTT GACAAATTTA TAGGGAGGGC CACTGTTCTT ACTGTTGCGC TACATCTGGC 2521 TATTCCGCTC AAATGGAAGT CAGACCGCAC GCCTGTGTGG ATTGACCAGT GGCCCCTTCC 2581 TGAAGGTAAA CTTGTAGCGC TAACGCAATT AGTGGAAAAA GAATTACAGT TAGGACATAT 2641 AGAGCCCTCA CTTAGTTGTT GGAACACACC TGTTTTTCGT GATCCGGAAG GCTTCCGGGT 2701 CTTATCGCTT ATTGCATGAT TTGCGCGCTG TTAACGCCAA GCTTGTCCCT TTTGGGGCCG 2761 TCCAACAGGG GGCGCCAGTT CTCTCCGCGC TCCCGCGTGG CTGGCCCCTG ATGGTCCTAG 2821 ACCTCAAGGA TTGCTTCTTT TCTATCCCTC TTGCGGAACA AGATCGCGAA GCTTTTGCAT 2881 TTACGCTCCC CTCTGTGAAT AACCAGGCCC CCGCTCGAAG ATTCCAATGG AAGGTCTTGC 2941 CCCAAGGGAT GACCTGTTCT CCCACTATCT GTCAGTTGGT AGTGGGTCAG GTGCTCGAGC 3001 CCTTGCGACT CAAGCACCCA GCTCTGCGCA TGTTGCATTA TATGGACGAT CTTTTGCTAG 3061 CCGCCTCAAG TCATGATGGG TTGGAAGCGG CAGGGAAGGA GGTTATCGGT ACATTGGAAA 3121 GAGCCGGGTT CACTATTTCG CCGGATAAGA TCCAGAGGGA GCCCGGAGTA CAATATCTTG 3181 GGTACAAGTT AGGCAGTACG TATGTAGCAC CCGTAGGCTT GGTAGCAGAA CCCAGGATAG 3241 CCACCTTGTG GGATGTTCAA AAGCTGGTGG GGTCACTTCA GTGGCTTCGC CCAGCGTTAG 3301 GGATCCCGCC ACGACTGATG GGTCCCTTTT ATGAGCAGTT ACGAGGGTCA GATCCTAACG 3361 AGGCGAGGGA ATGGAATCTA GACATGAAAA TGGCCTGGAG AGAGATCGTA CAGCTTAGCA 3421 CTACTGCTGC CTTGGAACGA TGGGACCCTG CCCAGCCTCT GGAAGGAGCG GTCGCTAGAT 3481 GTGAACAGGG GGCAATAGGG GTCCTGGGAC AGGGACTGTC CACACACCCA AGGCCATGTT 3541 TGTGGTTATT CTCCACCCAA CCCACCAAGG CGTTTACTGC TTGGTTAGAA GTGCTCACCC 3601 TTTTGATTAC TAAGCTACGC GCTTCGGCAG TGCGAACCTT TGGCAAGGAG GTTGATATCC 3661 TCCTGTTGCC TGCATGCTTC CGGGAGGACC TTCCGCTCCC GGAGGGGATC CTGTTAGCAC 3721 TTAGGGGGTT TGCAGGAAAA ATCAGGAGTA GTGACACGCC ATCTATTTTT GACATTGCGC 3781 GTCCACTGCA TGTTTCTCTG AAAGTGAGGG TTACCGACCA CCCTGTGCCG GGACCCACTG 3841 TCTTTACCGA CGCCTCCTCA AGCACCCATA AAGGGGTGGT AGTCTGGAGG GAGGGCCCAA 3901 GGTGGGAGAT AAAAGAAATA GTTGATTTGG GGGCAAGTGT ACAACAACTG GAGGCACGCG 3961 CTGTGGCCAT GGCACTTCTG CTGTGGCCGA CAACGCCCAC TAATGTAGTG ACTGACTCTG 4021 CGTTTGTTGC GAAAATGTTA CTCAAGATGG GACAGGAGGG AGTCCCGTCT ACAGCGGCAG 4081 CTTTTATTTT AGAGGATGCG TTAAGCCAAA GGTCAGCCAT GGCCGCCGTT CTCCACGTGC 4141 GGAGTCATTC TGAAGTGCCA GGGTTTTTCA CAGAAGGAAA TGACGTGGCA GATAGCCAAG 4201 CCACCTTTCA AGCGTATCCC TTGAGAGAGG CTAAAGATCT TCATACCGCT CTCCATATTG 4261 GACCCCGCGC GCTATCCAAA GCGTGTAATA TATCTATGCA GCAGGCTAGG GAGGTTGTTC 4321 AGACCTGCCC GCATTGTAAT TCAGCCCCTG CGTTGGAGGC CGGGGTAAAC CCTAGGGGTT 4381 TGGGACCCCT ACAGATATGG CAGACAGACT TTACGCTTGA GCCTAGAATG GCTCCCCGTT 4441 CCTGGCTCGC TGTTACTGTG GACACCGCCT CATCAGCGAT AGTCGTAACT CAGCATGGCC 4501 GTGTTACATC GGTTGCTGCA CAACATCATT GGGCCACGGC TATCGCCGTT TTGGGAAGAC 4561 CAAAGGCCAT AAAAACAGAT AACGGGTCCT GCTTCACGTC TAGATCCACG CGGGAGTGGC 4621 TCGCGAGATG GGGGATAGCA CACACCACCG GGATTCCGGG AAATTCCCAG GGTCAAGCTA 4681 TGGTAGAGCG GGCCAACCGG CTCCTGAAAG ATAAGATCCG TGTGCTTGCG GAGGGGGACG 4741 GCTTTATGAA AAGAATCCCC GCCAGCAAAC AGGGGGAACT ACTAGCCAAG GCAATGTATG 4801 CCCTCAATCA CTTTGAGCGT GGTGAAAACA CAAAAACACC GGTACAAAAA CACTGGAGAC 4861 CTACCGTTCT TACAGAAGGA CCCCCGGTTA AAATACGAAT AGAGACAGGG GAGTGGGAAA 4921 AAGGATGGAA CGTGCTGGTC TGGGGACGAG GTTATGCCGC TGTGAAAAAC AGGGACACTG 4981 ATAAGGTTAT TTGGGTACCC TCTCGGAAAG TTAAACCGGA TGTCACCCAA AAGGATGAGG 5041 TGACTAAGAA AGATGAGGCG AGCCCTCTTT TTGCAGGCAT TTCTGACTGG ATACCCTGGG 5101 AAGACGAGCA AGAAGGACTC CAAGGAGAAA CCGCTAGCAA CAAGCAAGAA AGACCCGGAG 5161 AAGACACCCT TGCTGCCAAC GAGAGTTAAT TATATTCTCA TTATTGGTGT CCTGGTCTTG 5221 TGTGAGGTTA CGGGGGTAAG AGCTGATGTC CACTTACTCG AGCAGCCAGG GAACCTTTGG 5281 ATTACATGGG CCAACCGTAC AGGCCAAACG GATTTTTGCC TCTCTACACA GTCAGCCACC 5341 TCCCCTTTTC AAACATGTTT GATAGGTATC CCGTCCCCTA TTTCCGAGGG TGATTTTAAG 5401 GGATATGTTT CTGATACAAA TTGCACCACC TTGGGAACTG ATCGGTTAGT CTCGTCAGCC 5461 GACTTTACTG GCGGACCTGA CAACAGTACC ACCCTCACTT ATCGGAAGGT CTCATGCTTG 5521 TTGTTAAAGC TGAATGTCTC TATGTGGGAT GAGCCACCTG AACTACAGCT GTTAGGTTCC 5581 CAGTCTCTCC CTAACATTAC TAATATTGCT CAGATTTCCG GTATAACCGG GGGATGCGTA 5641 GGCTTCAGAC CACAAGGGGT TCCTTGGTAT CTAGGTTGGT CTAGACAGGA GGCCACGCGG 5701 TTTCTCCTTA GACACCCCTC TTTCTCTAAA TCCACGGAAC CGTTTACAGT GGTGACAGCG 5761 GATAGGCACA ATCTTTTTAT GGGGAGTGAG TACTGCGGTG CATATGGCTA CAGATTTTGG 5821 AACATGTATA ACTGCTCACA GGTGGGGCGG CAGTACCGCT GTGGTAATGC GCGCACGCCC 5881 CGCACGGGTC TTCCTGAAAT CCAGTGTACA AGGAGAGGAG GCAAATGGGT TAATCAATCA 5941 CAGGAAATTA ATGAGTCGGA GCCGTTCAGC TTTACGGTGA ACTGTACAGC TAGTAGTTTG 6001 GGTAATGCCA GTGGGTGTTG CGGAAAAGCA GGCACGATTC TCCCGGGAAA GTGGGTCGAC 6061 AGCACACAAG GTAGTTTCAC CAAACCAAAA GCGCTACCAC CCGCAATTTT CCTCATTTGT 6121 GGGGATCGCG CATGGCAAGG AATTCCCAGT CGTCCGGTAG GGGGCCCCTG CTATTTAGGC 6181 AAGCTTACCA TGTTAGCACC TAAGCATACA GATATTCTCA AGGTGCTTGT CAATTCATCG 6241 CGGACAGGTA TAAGACGTAA ACGAAGCACC TCACACCTGG ATGATACATG CTCAGATGAA 6301 GTGCAGCTTT GGGGTCCTAC AGCAAGAATC TTTGCATCTA TCCTAGCCCC GGGGGTAGCA 6361 GCTGCGCAAG CCTTAAGAGA AATTGAGAGA CTAGCCTGTT GGTCCGTTAA ACAGGCTAAC 6421 TTGACAACAT CACTCCTCGG GGACTTATTG GATGATGTCA CGAGTATTCG ACACGCGGTC 6481 CTGCAGAACC GAGCGGCTAT TGACTTCTTG CTCCTAGCTC ACGGCCATGG CTGTGAGGAC 6541 GTTGCCGGAA TGTGCTGTTT CAATTTGAGT GATCAGAGTG AGTCTATACA GAAGAAGTTC 6601 CAGCTAATGA AGGAACATGT CAATAAGATC GGCGTGGATA GCGACCTAAT TGGAAGTTGG 6661 CTGCGAGGAC TATTCGGGGG AATAGGAGAA TGGGCCGTTC ATTTGCTGAA AGGACTGCTT 6721 TTGGGGCTTG TAGTTATTTT GTTGCTAGTA GTGTGCCTGC CTTGCCTTTT GCAAATGTTA 6781 TGCGGTAATA GGAGAAAGAT GATTAATAAC TCCATCAGCT ACCACACGGA ATATAAGAAG 6841 CTGCAAAAGG CCTGTGGGCA GCCTGAAAGC AGAATAGTAT AAGGCAGTAC ATGGGTGGTG 6901 GTATAGCGCT TGCGAGTCCA TCGAGCAAGG CAGGAAAGAC AGCTATTGGT AATTGTGAAA 6961 TACGCTTTTG TCTGTGTGCT GCAGGAGCTG AGCTGACTCT GCTGGTGGCC TCGCGTACCA 7021 CTGTGGCATC GGCGGCCGCG TTTATCAACA AGTTTGTACA AAAAAGCTGA ACGAGAAACG 7081 TAAAATGATA TAAATATCAA TATATTAAAT TAGATTTTGC ATAAAAAACA GACTACATAA 7141 TACTGTAAAA CACAACATAT CCAGTCACTA TGGCGGCCGC ATTAGGCACC CCAGGCTTTA 7201 CACTTTATGC TTCCGGCTCG TATAATGTGT GGATTTTGAG TTAGGATCCG TCGAGATTTT 7261 CAGGAGCTAA GGAAGCTAAA ATGGAGAAAA AAATCACTGG ATATACCACC GTTGATATAT 7321 CCCAATGGCA TCGTAAAGAA CATTTTGAGG CATTTCAGTC AGTTGCTCAA TGTACCTATA 7381 ACCAGACCGT TCAGCTGGAT ATTACGGCCT TTTTAAAGAC CGTAAAGAAA AATAAGCACA 7441 AGTTTTATCC GGCCTTTATT CACATTCTTG CCCGCCTGAT GAATGCTCAT CCGGAATTCC 7501 GTATGGCAAT GAAAGACGGT GAGCTGGTGA TATGGGATAG TGTTCACCCT TGTTACACCG 7561 TTTTCCATGA GCAAACTGAA ACGTTTTCAT CGCTCTGGAG TGAATACCAC GACGATTTCC 7621 GGCAGTTTCT ACACATATAT TCGCAAGATG TGGCGTGTTA CGGTGAAAAC CTGGCCTATT 7681 TCCCTAAGGG TTTATTGAGA ATATGTTTTT CGTCTCAGCC AATCCTGGGT GAGTTTCACC 7741 AGTTTTGATT TAAACGTGGC CAATATGGAC AACTTCTTCG CCCCCGTTTT CACCATGGGC 7801 AAATATTATA CGCAAGGCGA CAAGGTGCTG ATGCCGCTGG CGATTCAGGT TCATCATGCC 7861 GTTTGTGATG GCTTCCATGT CGGCAGAATG CTTAATGAAT TACAACAGTA CTGCGATGAG 7921 TGGCAGGGCG GGGCGTAAAC GCGTGGATCC GGCTTACTAA AAGCCAGATA ACAGTATGCG 7981 TATTTGCGCG CTGATTTTTG CGGTATAAGA ATATATACTG ATATGTATAC CCGAAGTATG 8041 TCAAAAAGAG GTATGCTATG AAGCAGCGTA TTACAGTGAC AGTTGACAGC GACAGCTATC 8101 AGTTGCTCAA GGCATATATG ATGTCAATAT CTCCGGTCTG GTAAGCACAA CCATGCAGAA 8161 TGAAGCCCGT CGTCTGCGTG CCGAACGCTG GAAAGCGGAA AATCAGGAAG GGATGGCTGA 8221 GGTCGCCCGG TTTATTGAAA TGAACGGCTC TTTTGCTGAC GAGAACAGGG GCTGGTGAAA 8281 TGCAGTTTAA GGTTTACACC TATAAAAGAG AGAGCGTTAT CGTCTGTTTG TGGATGTACA 8341 GAGTGATATT ATTGACACGC CCGGGCGACG GATGGTGATC CCCCTGGCCA GTGCACGTCT 8401 GCTGTCAGAT AAAGTCTCCC GTGAACTTTA CCCGGTGGTG CATATCGGGG ATGAAAGCTG 8461 GCGCATGATG ACCACCGATA TGGCCAGTGT GCCGGTCTCC GTTATCGGGG AAGAAGTGGC 8521 TGATCTCAGC CACCGCGAAA ATGACATCAA AAACGCCATT AACCTGATGT TCTGGGGAAT 8581 ATAAATGTCA GGCTCCCTTA TACACAGCCA GTCTGCAGGT CGACCATAGT GACTGGATAT 8641 GTTGTGTTTT ACAGTATTAT GTAGTCTGTT TTTTATGCAA AATCTAATTT AATATATTGA 8701 TATTTATATC ATTTTACGTT TCTCGTTCAG CTTTCTTGTA CAAAGTGGTG ATAAACTTAA 8761 TTAAATTTAA ATATCGATGC GATGTACGGG CCAGATATAC GCGTATCTGA GGGGACTAGG 8821 GTGTGTTTAG GCGAAAAGCG GGGCTTCGGT TGTACGCGGT TAGGAGTCCC CTTAGGATAT 8881 AGTAGTTTCG CTTTTGCATA GGGAGGGGGA AATGTAGTCT TATGCAATAC TCTTGTAGTC 8941 TTGCAACATG GTAACGATGA GTTAGCAACA TGCCTTACAA GGAGAGAAAA AGCACCGTGC 9001 ATGCCGATTG GTGGAAGTAA GGTGGTACGA TCGTGCCTTA TTAGGAAGGC AACAGACGGG 9061 TCTGACATGG ATTGGACGAA CCACTGAATT CCGCATTGCA GAGATATTGT ATTTAAGTGC 9121 CTAGCTCGAT ACAATAAACG CCATTTGACC ATTCACCACA TTGGTGTGCA CCTGGGTTGA 9181 TGGCCGGACC GTTGATTCCC TGACGACTAC GAGCACCTGC ATGAAGCAGA AGGCTTCATT 9241 TGGTGACCCC GACGTGATAG TTAGGGAATA GTGGTCGGCC ACAGACGGCG TGGCGATCCT 9301 GTCTCCATCC GTCTCGTCTA TCGGGAGGCG AGTTCGATGA CCCTGGTGGA GGGGGCTGCG 9361 GCTTAGGGAG GCAGAAGCTG AGTACCGTCG GAGGGGATCC ACAGGACGGG TGTGGTCGCC 9421 ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA 9481 AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC 9541 GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG 9601 CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG 9661 ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC 9721 TGTGATAAAC TACCGCATTA AAGCTTCCAA ACTTGGCTGT TTCCTGTGTG AAATTGTTAT 9781 CCGCTCACAA TTCCACACAT TATACGAGCC GGAAGCATAA AGTGTAAAAC CTGGGGTGCC 9841 TAATGAGTGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA 9901 ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG 9961 GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT 10021 AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC 10081 GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA 10141 CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC 10201 TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA 10261 TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG 10321 AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA 10381 CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA 10441 TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA 10501 CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC 10561 TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA 10621 CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG 10681 ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT 10741 GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC 10801 TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA 10861 CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT 10921 AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT 10981 TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG 11041 AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC 11101 CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG 11161 TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG 11221 CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT 11281 CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG 11341 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC 11401 GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG 11461 AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG 11521 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG 11581 GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC 11641 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT 11701 TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC 11761 CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC 11821 GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT 11881 TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATG GTGCACTCTC AGTACAATCT 11941 GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT 12001 GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC 12061 GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC 12121 ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG 12181 CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT 12241 TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC 12301 TTGATGCCTC CGTGTAAGGG GGAATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA 12361 GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT 12421 GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA 12481 TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG 12541 ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA 12601 ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG 12661 TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC 12721 CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA 12781 ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC 12841 TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT 12901 GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC 12961 TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG ATGCGATGTA 13021 CGGGCCAGAT ATACGCGTAT CTGAGGGGAC TAGGGTGTGT TTAGGCGAAA AGCGGGGCTT 13081 CGGTTGTACG CGGTTAGGAG TCCCCTTAGG ATATAGTAGT TTCGCTTTTG CATAGGGAGG 13141 GGGAAATGTA GTCTTATGCA ATACTCTTGT AGTCTTGCAA CATGGTAACG ATGAGTTAGC 13201 AACATGCCTT ACAAGGAGAG AAAAAGCACC GTGCATGCCG ATTGGTGGAA GTAAGGTGGT 13261 ACGATCGTGC CTTATTAGGA AGGCAACAGA CGGGTCTGAC ATGGATTGGA CGAACCACTG 13321 AATTCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AAC // =4 RCASBP-Y DV "Times New Roman k"C=4t ORF3"Times New Romank"C=4.4 pREP_f_pri"Times New Romank"CɆ=4 EORF3"Times New Romank"C=4 ORF2"Times New Romank"=4ORF1"Times New Romank"C=4attR1"Times New Romank"C=4attR2"Times New Romank"CɆ=44lac_pro"Times New Romank"=4qCAT/CamR(variant)"Times New Romank"C=4{ !ccdB"Times New Romank"CɆ=4!"attR1"Times New Romank"C=4!"attR2"Times New Roman*k"=4## pREP_f_pri"Times New Romank"C=4$%tet"Times New Romank"C=4"&9& M13_pUC_r"Times New Romank"CɆ=4'$'AmpR_pro"Times New Romank"=4N'*ORF3"Times New Romank"C=4N'* Ampicillin"Times New Romank"C=4E+- pBR322_origin"Times New Romank"CɆ=4=/S/ pGEX_3_pri"Times New Romank"=4M/ 0ROP"Times New Roman k"SacI"Times New Roman=4+lXD5D k"SacII"Times New Roman=4HWee k"HpaI"Times New Roman  =4v k"PmlI"Times New Roman(=4q k"AgeI"Times New Roman=4M]ll k"KpnI"Times New Roman =4CR`` k"AflII"Times New Roman =42EET k"StuI"Times New Roman!=4 4GvGV k"test"Times New Romanx'=4BA<~a k"test"Times New Roman>"=4b8 k"test"Times New Roman&=4up-