Retroviral Plasmids
MDH1-PGK-GFP_2.0 to RCASBP-Y DV | V207 pRETRO-Triple-Flag SD - Acceptor to V207 pRETRO-Triple-Flag SD - Acceptor |
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| Plasmids 0 - 4 | Descriptions |
 | MDH1-PGK-GFP_2.0 - Gene/insert name: None ;
Vector backbone: MDH1-PGK-GFP 2.0;
5' Sequencing primer: EGFP-C ;
3' Sequencing primer: ggatcccaatatttgcatgtcgc ;
Bacteria resistance: Ampicillin ;
High or low copy: High Copy ;
Grow in standard E. coli @ 37C: Yes ;
Plasmid Provided In: DH5a ;
Principal Investigator: Chang-Zheng Chen ;
Article: MicroRNAs modulate hematopoietic linea... |
 | pBABE-hygro - Gene/insert name: none; Vector backbone: pBABE-hygro (Search Vector Database); 5' Sequencing primer: pBABE 5' (List of Sequencing Primers); 3' Sequencing primer: pBABE 3'; Bacteria resistance: Ampicillin; High or low copy: High Copy; Grow in standard E. coli @ 37C: Yes; Selectable markers: Hygromycin; Plasmid Provided In: DH5a; Principal Investigator: Bob Weinberg; Comment... |
 | pBABE-puro - Gene/insert name: none; Vector backbone: pBABE-puro (Search Vector Database); 5' Sequencing primer: pBABE 5' (List of Sequencing Primers); 3' Sequencing primer: pBABE 3'; Bacteria resistance: Ampicillin; High or low copy: High Copy; Grow in standard E. coli @ 37C: Yes; Selectable markers: Puromycin; Plasmid Provided In: DH5a; Principal Investigator: Bob Weinberg; Comments: M... |
 | pCLXSN - Gene/insert name: None ;
Vector backbone: pCLXSN ;
Backbone manufacturer: Verma Lab ;
5' Sequencing primer: LXSN primer;
Bacteria resistance: Ampicillin ;
High or low copy: High Copy ;
Grow in standard E. coli @ 37C: Yes ;
Selectable markers: Neomycin ;
Plasmid Provided In: DH5a ;
Principal Investigator: Inder M Verma ;
Comments: A 3.1-kb fragment of LXSN, ... |
 | RCASBP-Y DV - Gene/insert name: Gateway ccdB reading frame cassette ;
Vector backbone: RCASBP-Y;
Cloning site 5': PmeI ;
Site destroyed during cloning: Yes ;
Cloning site 3': PmeI ;
Site destroyed during cloning: Yes ;
5' Sequencing primer: gagctgagctgactctgctggtgg c ;
3' Sequencing primer: cagatacgcgtatatctggc ;
Bacteria resistance: Ampicillin,Chloramphenicol ;
High... |
| Plasmids 5 - 9 | Descriptions |
 | V207 pRETRO-Triple-Flag SD - Acceptor - Gene/insert name: None ;
Relevant mutations/deletions: Splice for 5' Tags ;
Fusion proteins or tags: 3xFLAG ;
Terminal: N terminal on backbone ;
Vector backbone: NA ;
5' Sequencing primer: MSCV ;
Bacteria resistance: Ampicillin ;
High or low copy: High Copy ;
Grow in standard E. coli @ 37C: Yes ;
Selectable markers: Pur... |
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