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V207 pRETRO-Triple-Flag SD - Acceptor
Gene/insert name: None
Insert size (bp): Unknown
Relevant mutations/deletions: Splice for 5' Tags
Fusion proteins or tags: 3xFLAG
Terminal: N terminal on backbone
Vector backbone: NA
Type of vector: Mammalian expression,Retroviral,Cre/Lox,Acceptor vector
Backbone size (bp): 6912
5' Sequencing primer: MSCV
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Selectable markers: Puromycin
Plasmid Provided In: DH5a
Principal Investigator: Tony Pawson
Comments: Acceptor vector for the Creator Splice system.
Article: Modification of the Creator recombination system for proteomics applications--improved expression by addition of splice sites. Colwill K et al. (BMC Biotechnol. 2006 . 6():13. Pubmed)