Sequence of V207

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Sequence of V207_pRETRO

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Sequence of V207 pRETRO-Triple-Flag SD - Acceptor : 6912 BP; 1574 A; 1936 C; 1840 G; 1562 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat 60

1 actttctggg gtggacatcc aaaccgttcg atcgaattca ttgcggtaaa acgttccgta 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca 120

61 ccttttatgt attgactctt atctcttcaa gtctagttcc aatccttgtc tctctgtcgt 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga 180

121 cttatacccg gtttgtccta tagacaccat tcgtcaagga cggggccgag tcccggttct 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt 240

181 tgtctaccag gggtctacgc cagggcggga gtcgtcaaag atctcttggt agtctacaaa 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc 300

241 ggtcccacgg ggttcctgga ctttactggg acacggaata aacttgattg gttagtcaag 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc 360

301 cgaagagcga agacaagcgc gcgaagacga ggggctcgag ttattttctc gggtgttggg 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata 420

361 gagtgagccg cgcggtcagg aggctatctg acgcagcggg cccatgggca taagggttat 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca 480

421 ttcggagaac gacaaacgta ggcttagcac ctgagcgact aggaaccctc ccagaggagt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga 540

481 ctaactaact gacgggtgga gcccccagaa agtaaacctc caaggtggct ctaaacctct 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg 600

541 ggggacgggt ccctggtggc tgggggggcg gccctccatt cgaccggtcg ccagcaaagc 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt 660

601 acagacagag acagaaacac gcacaaacac ggccgtagat tacaaacgcg gacgcagaca 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa 720

661 tgatcaatcg attgatcgag acatagaccg cctgggcacc accttgactg ctcaagactt 720

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga 780

721 gtgggccggc gttgggaccc tctgcagggt ccctgaaacc cccggcaaaa acaccgggct 780

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag 840

781 ggactccttc cctcagctac accttaggct ggggcagtcc tatacaccaa gaccatcctc 840

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa 900

841 tgctcttgga ttttgtcaag ggcggaggca gacttaaaaa cgaaagccaa accttggctt 900

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg 960

901 cggcgcgcag aacagacgac gtcgcgacgt cgtagcaaga cacaacagag acagactgac 960

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct 1020

961 acaaagacat aaacagactt ttaatcccgg tctgacaatg gtgagggaat tcaaactgga 1020

.......... ........ psi_plus_pack ........ gag ..........

.......... .......... .......... .......... .......... ..........

1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga 1080

1021 atccagtgac ctttctacag ctcgcctagc gagtgttggt cagccatcta cagttcttct 1080

.......... ...... psi_plus_pack gag ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag 1140

1081 ctgcaaccca atggaagacg agacgtctta ccggttggaa attgcagcct accggcgctc 1140

.......... ...... psi_plus_pack gag ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc 1200

1141 tgccgtggaa attggctctg gagtagtggg tccaattcta gttccagaaa agtggaccgg 1200

.......... ...... psi_plus_pack gag ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc 1260

1201 gcgtacctgt gggtctggtc caggggatgt agcactggac ccttcggaac cgaaaactgg 1260

.......... ...... psi_plus_pack gag ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg 1320

1261 ggggagggac ccagttcggg aaacatgtgg gattcggagg cggaggagaa ggaggtaggc 1320

.......... ...... psi_plus_pack gag ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag 1380

1321 ggggcagaga gggggaactt ggaggagcaa gctggggcgg agctaggagg gaaataggtc 1380

... psi_plus_pack gag MSCV_primer pLXSN_5_primer . pBABE_5_primer

.......... .......... .......... XhoI(1416) .......... ..........

1381 ccctcactcc ttctctaggc gccggaatta gatctctcga gccacatgga ctacaaggac 1440

1381 gggagtgagg aagagatccg cggccttaat ctagagagct cggtgtacct gatgttcctg 1440

gag .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cacgacggcg actacaagga ccacgacatc gactacaagg acgacgacga caaggtaagt 1500

1441 gtgctgccgc tgatgttcct ggtgctgtag ctgatgttcc tgctgctgct gttccattca 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 agatcttgac ctctcaaaag cgggcatgac ttctgcgcta agattgtcag tttccaaaaa 1560

1501 tctagaactg gagagttttc gcccgtactg aagacgcgat tctaacagtc aaaggttttt 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cgaggaggat ttgatattca cctggcccgc ggtgatgcct ttgagggtgg ccgcatccat 1620

1561 gctcctccta aactataagt ggaccgggcg ccactacgga aactcccacc ggcgtaggta 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ctggtcagaa aagacaatct ttttgttgtc aagcttggtg gcaaacgacc cgtagagggc 1680

1621 gaccagtctt ttctgttaga aaaacaacag ttcgaaccac cgtttgctgg gcatctcccg 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... NruI(1726) ..........

1681 gttggacagc aacttggcga tggagcgcag ggtttggttt ttgtcgcgat cggcgcgctc 1740

1681 caacctgtcg ttgaaccgct acctcgcgtc ccaaaccaaa aacagcgcta gccgcgcgag 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cttggccgcg atgtttagct gcacgtattc gcgcgcaacg caccgccatt cgggaaagac 1800

1741 gaaccggcgc tacaaatcga cgtgcataag cgcgcgttgc gtggcggtaa gccctttctg 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ggtggtgcgc tcgtcgggca ccaggtgcac gcgccaacag cggttgtgca gggtgacaag 1860

1801 ccaccacgcg agcagcccgt ggtccacgtg cgcggttgtc gccaacacgt cccactgttc 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gtcaacgcta agcttcgata acttcgtata gcatacatta tacgaagtta tagatccaat 1920

1861 cagttgcgat tcgaagctat tgaagcatat cgtatgtaat atgcttcaat atctaggtta 1920

.......... .......... .......... loxP .......... ..........

.......... .......... .......... .......... .......... ..........

1921 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 1980

1921 taataacttc gtaaatagtc ccaataacag agtactcgcc tatgtataaa cttacataaa 1980

.......... .......... ........ AmpR_promoter ........ ..........

.......... .......... .......... .......... .......... ..........

1981 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtgt 2040

1981 tctttttatt tgtttatccc caaggcgcgt gtaaaggggc ttttcacggt ggactgcaca 2040

.......... .......... .......... .......... .......... ..........

HpaI(2041) EcoRI(2045) ........ .......... .......... ..........

2041 taacgaattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt 2100

2041 attgcttaag atggcccatc ccctccgcga aaagggttcc gtcagacctc gtacgcgaaa 2100

.......... .......... .......... .......... ....... MSCV_rev_primer

.......... .......... .......... .......... .......... ..........

2101 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac 2160

2101 tcgtcggggc gacccgtgaa ccgcgatgtg ttcaccggag accggagcgt gtgtaaggtg 2160

MSCV_rev_primer .... .......... .......... .......... ..........

AgeI(2165) .......... .......... .......... .......... ..........

2161 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac 2220

2161 taggtggcca tccgcggttg gccgaggcaa gaaaccaccg gggaagcgcg gtggaagatg 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca 2280

2221 aggaggggat cagtccttca agggggggcg gggcgtcgag cgcagcacgt cctgcactgt 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa 2340

2281 ttaccttcat cgtgcagagt gatcagagca cgtctacctg tcgtggcgac tcgttacctt 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca 2400

2341 cgcccatccg gaaaccccgt cgccggttat cgtcgaaacg aggaagcgaa agacccgagt 2400

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg 2460

2401 ctccgaccct tccccaccca ggcccccgcc cgagtccccg cccgagtccc cgccccgccc 2460

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc 2520

2461 gcgggcttcc aggaggcctc cgggccgtaa gacgtgcgaa gttttcgcgt gcagacggcg 2520

.......... ....... Orf frame 1 mPGK_F_primer ....... ..........

.......... .......... .......... .......... .......... ..........

2521 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc 2580

2521 cgacaagagg agaaggagta gaggcccgga aagctggacg tcgggttcga atggtactgg 2580

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc 2640

2581 ctcatgttcg ggtgccacgc ggagcggtgg gcgctgctgc aggggtcccg gcatgcgtgg 2640

.......... .. Orf frame 1 puro(variant) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac 2700

2641 gagcggcggc gcaagcggct gatggggcgg tgcgcggtgt ggcagctagg cctggcggtg 2700

.......... .. Orf frame 1 puro(variant) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2701 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 2760

2701 tagctcgccc agtggctcga cgttcttgag aaggagtgcg cgcagcccga gctgtagccg 2760

.......... .. Orf frame 1 puro(variant) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2761 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 2820

2761 ttccacaccc agcgcctgct gccgcggcgc caccgccaga cctggtgcgg cctctcgcag 2820

.......... .. Orf frame 1 puro(variant) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 2880

2821 cttcgccccc gccacaagcg gctctagccg ggcgcgtacc ggctcaactc gccaagggcc 2880

.......... .. Orf frame 1 puro(variant) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg 2940

2881 gaccggcgcg tcgttgtcta ccttccggag gaccgcggcg tggccgggtt cctcgggcgc 2940

.......... .. Orf frame 1 puro(variant) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 3000

2941 accaaggacc ggtggcagcc gcagagcggg ctggtggtcc cgttcccaga cccgtcgcgg 3000

.......... .. Orf frame 1 puro(variant) .. .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 3060

3001 cagcacgagg ggcctcacct ccgccggctc gcgcggcccc acgggcggaa ggacctctgg 3060

.......... .. Orf frame 1 puro(variant) .. .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 3120

3061 aggcgcgggg cgttggaggg gaagatgctc gccgagccga agtggcagtg gcggctgcag 3120

.......... .. Orf frame 1 puro(variant) .. .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg 3180

3121 ctccacgggc ttcctggcgc gtggaccacg tactgggcgt tcgggccacg gactgcgggc 3180

.......... .. Orf frame 1 puro(variant) .. .......... ..........

.......... .......... .......... .......... ClaI(3229) ..........

3181 ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc gataaaataa 3240

3181 ggggtgctgg gcgtcgcggg ctggctttcc tcgcgtgctg gggtacgtag ctattttatt 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc 3300

3241 ttctaaaata aatcagaggt ctttttcccc ccttactttc tggggtggac atccaaaccg 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 aagctagctt aagtaacgcc attttgcaag gcatggaaaa tacataactg agaatagaga 3360

3301 ttcgatcgaa ttcattgcgg taaaacgttc cgtacctttt atgtattgac tcttatctct 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 agttcagatc aaggttagga acagagagac agcagaatat gggccaaaca ggatatctgt 3420

3361 tcaagtctag ttccaatcct tgtctctctg tcgtcttata cccggtttgt cctatagaca 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtcccg 3480

3421 ccattcgtca aggacggggc cgagtcccgg ttcttgtcta ccaggggtct acgccagggc 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg 3540

3481 gggagtcgtc aaagatctct tggtagtcta caaaggtccc acggggttcc tggactttac 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc 3600

3541 tgggacacgg aataaacttg attggttagt caagcgaaga gcgaagacaa gcgcgcgaag 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tgctccccga gctcaataaa agagcccaca acccctcact cggcgcgcca gtcctccgat 3660

3601 acgaggggct cgagttattt tctcgggtgt tggggagtga gccgcgcggt caggaggcta 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 agactgcgtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt 3720

3661 tctgacgcag cgggcccatg ggcacatagg ttatttggga gaacgtcaac gtaggctgaa 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg 3780

3721 caccagagcg acaaggaacc ctcccagagg agactcacta actgatgggc agtcgccccc 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tctttcatgg gtaacagttt cttgaagttg gagaacaaca ttctgagggt aggagtcgaa 3840

3781 agaaagtacc cattgtcaaa gaacttcaac ctcttgttgt aagactccca tcctcagctt 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tattaagtaa tcctgactca attagccact gttttgaatc cacatactcc aatactcctg 3900

3841 ataattcatt aggactgagt taatcggtga caaaacttag gtgtatgagg ttatgaggac 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 aaatagttca ttatggacag cgcagaagag ctggggagaa ttaattcgta atcatggtca 3960

3901 tttatcaagt aatacctgtc gcgtcttctc gacccctctt aattaagcat tagtaccagt 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 4020

3961 atcgacaaag gacacacttt aacaataggc gagtgttaag gtgtgttgta tgctcggcct 4020

M13_reverse_primer M13_pUC_rev_primer .... ......... lac_promoter

.......... .......... .......... .......... .......... ..........

4021 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 4080

4021 tcgtatttca catttcggac cccacggatt actcactcga ttgagtgtaa ttaacgcaac 4080

lac_promoter ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 4140

4081 gcgagtgacg ggcgaaaggt cagccctttg gacagcacgg tcgacgtaat tacttagccg 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 4200

4141 gttgcgcgcc cctctccgcc aaacgcataa cccgcgagaa ggcgaaggag cgagtgactg 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 4260

4201 agcgacgcga gccagcaagc cgacgccgct cgccatagtc gagtgagttt ccgccattat 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 4320

4261 gccaataggt gtcttagtcc cctattgcgt cctttcttgt acactcgttt tccggtcgtt 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 4380

4321 ttccggtcct tggcattttt ccggcgcaac gaccgcaaaa aggtatccga ggcgggggga 4380

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

4381 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 4440

4381 ctgctcgtag tgtttttagc tgcgagttca gtctccaccg ctttgggctg tcctgatatt 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 4500

4441 tctatggtcc gcaaaggggg accttcgagg gagcacgcga gaggacaagg ctgggacggc 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 4560

4501 gaatggccta tggacaggcg gaaagaggga agcccttcgc accgcgaaag agtatcgagt 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 4620

4561 gcgacatcca tagagtcaag ccacatccag caagcgaggt tcgacccgac acacgtgctt 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 4680

4621 ggggggcaag tcgggctggc gacgcggaat aggccattga tagcagaact caggttgggc 4680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 4740

4681 cattctgtgc tgaatagcgg tgaccgtcgt cggtgaccat tgtcctaatc gtctcgctcc 4740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 4800

4741 atacatccgc cacgatgtct caagaacttc accaccggat tgatgccgat gtgatcttcc 4800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4801 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 4860

4801 tgtcataaac catagacgcg agacgacttc ggtcaatgga agcctttttc tcaaccatcg 4860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 4920

4861 agaactaggc cgtttgtttg gtggcgacca tcgccaccaa aaaaacaaac gttcgtcgtc 4920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4921 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 4980

4921 taatgcgcgt ctttttttcc tagagttctt ctaggaaact agaaaagatg ccccagactg 4980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 5040

4981 cgagtcacct tgcttttgag tgcaattccc taaaaccagt actctaatag tttttcctag 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 5100

5041 aagtggatct aggaaaattt aatttttact tcaaaattta gttagatttc atatatactc 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 5160

5101 atttgaacca gactgtcaat ggttacgaat tagtcactcc gtggatagag tcgctagaca 5160

.......... .......... .......... Ampicillin .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 5220

5161 gataaagcaa gtaggtatca acggactgag gggcagcaca tctattgatg ctatgccctc 5220

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 5280

5221 ccgaatggta gaccggggtc acgacgttac tatggcgctc tgggtgcgag tggccgaggt 5280

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 5340

5281 ctaaatagtc gttatttggt cggtcggcct tcccggctcg cgtcttcacc aggacgttga 5340

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 5400

5341 aataggcgga ggtaggtcag ataattaaca acggcccttc gatctcattc atcaagcggt 5400

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 5460

5401 caattatcaa acgcgttgca acaacggtaa cgatgtccgt agcaccacag tgcgagcagc 5460

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 5520

5461 aaaccatacc gaagtaagtc gaggccaagg gttgctagtt ccgctcaatg tactaggggg 5520

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 5580

5521 tacaacacgt tttttcgcca atcgaggaag ccaggaggct agcaacagtc ttcattcaac 5580

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 5640

5581 cggcgtcaca atagtgagta ccaataccgt cgtgacgtat taagagaatg acagtacggt 5640

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 5700

5641 aggcattcta cgaaaagaca ctgaccactc atgagttggt tcagtaagac tcttatcaca 5700

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 5760

5701 tacgccgctg gctcaacgag aacgggccgc agttatgccc tattatggcg cggtgtatcg 5760

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 5820

5761 tcttgaaatt ttcacgagta gtaacctttt gcaagaagcc ccgcttttga gagttcctag 5820

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 5880

5821 aatggcgaca actctaggtc aagctacatt gggtgagcac gtgggttgac tagaagtcgt 5880

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 5940

5881 agaaaatgaa agtggtcgca aagacccact cgtttttgtc cttccgtttt acggcgtttt 5940

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 6000

5941 ttcccttatt cccgctgtgc ctttacaact tatgagtatg agaaggaaaa agttataata 6000

.......... Ampicillin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 6060

6001 acttcgtaaa tagtcccaat aacagagtac tcgcctatgt ataaacttac ataaatcttt 6060

.......... .......... ........ AmpR_promoter ........ ..........

.......... .......... .......... .......... .......... ..........

6061 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa 6120

6061 ttatttgttt atccccaagg cgcgtgtaaa ggggcttttc acggtggact gcagattctt 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc 6180

6121 tggtaataat agtactgtaa ttggatattt ttatccgcat agtgctccgg gaaagcagag 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca 6240

6181 cgcgcaaagc cactactgcc acttttggag actgtgtacg tcgagggcct ctgccagtgt 6240

.......... .......... ........ pGEX_3_primer ........ ..........

.......... .......... .......... .......... .......... ..........

6241 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt 6300

6241 cgaacagaca ttcgcctacg gccctcgtct gttcgggcag tcccgcgcag tcgcccacaa 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac 6360

6301 ccgcccacag ccccgaccga attgatacgc cgtagtctcg tctaacatga ctctcacgtg 6360

.......... .......... .......... .......... .......... ..........

NdeI(6362) .......... .......... .......... .......... ..........

6361 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat 6420

6361 gtatacgcca cactttatgg cgtgtctacg cattcctctt ttatggcgta gtccgcggta 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta 6480

6421 agcggtaagt ccgacgcgtt gacaaccctt cccgctagcc acgcccggag aagcgataat 6480

.......... .......... .......... lacZ_a .......... ..........

.......... .......... .......... .......... .......... ..........

6481 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt 6540

6481 gcggtcgacc gctttccccc tacacgacgt tccgctaatt caacccattg cggtcccaaa 6540

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 tcccagtcac gacgttgtaa aacgacggcg caaggaatgg tgcatgcaag gagatggcgc 6600

6541 agggtcagtg ctgcaacatt ttgctgccgc gttccttacc acgtacgttc ctctaccgcg 6600

lacZ_a M13_pUC_fwd_primer .. .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga 6660

6601 ggttgtcagg gggccggtgc cccggacggt ggtatgggtg cggctttgtt cgcgagtact 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa 6720

6661 cgggcttcac cgctcgggct agaaggggta gccactacag ccgctatatc cgcggtcgtt 6720

.......... .......... .......... ....... pBRrevBam_primer .......

.......... .......... .......... .......... .......... ..........

6721 ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg cgattagtcc 6780

6721 ggcgtggaca ccgcggccac tacggccggt gctacgcagg ccgcatctcc gctaatcagg 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 aatttgttaa agacaggata tcagtggtcc aggctctagt tttgactcaa caatatcacc 6840

6781 ttaaacaatt tctgtcctat agtcaccagg tccgagatca aaactgagtt gttatagtgg 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 agctgaagcc tatagagtac gagccataga taaaataaaa gattttattt agtctccaga 6900

6841 tcgacttcgg atatctcatg ctcggtatct attttatttt ctaaaataaa tcagaggtct 6900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 aaaagggggg aa........ .......... .......... .......... .......... 6912

6901 ttttcccccc tt........ .......... .......... .......... .......... 6912

.......... .......... .......... .......... .......... ..........