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    Plasmid map of V44_pHIPPY_blunt

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    V44 pHIPPY blunt
    Gene/insert name: none
    Insert size (bp): Unknown
    Vector backbone: pHIPPY
    Backbone manufacturer: Moon Lab
    Type of vector: Mammalian expression,RNAi
    Backbone size (bp): 2167
    5' Sequencing primer: H1 primer
    Bacteria resistance: zeocin (50ug/mL)
    High or low copy: High Copy
    Grow in standard E. coli @ 37C: Yes
    Selectable markers: Zeocin
    Plasmid Provided In: DH5a
    Principal Investigator: Randall Moon
    Comments: This construct is a blunt BsmB1 site version, for use as a NEGATIVE CONTROL for pHippy targetting of siRNAs in mammalian cells.
    Article: A plasmid-based system for expressing small interfering RNA libraries in mammalian cells. Kaykas A et al. (BMC Cell Biol. 2004 Apr 30. 5():16. Pubmed)

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