Sequence of V44_pHIPPY

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Sequence of V44_pHIPPY_blunt

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Sequence of V44 pHIPPY blunt : 2145 BP; 485 A; 597 C; 577 G; 486 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 caattcgcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 60

1 gttaagcggt ccttggcatt tttccggcgc aacgaccgca aaaaggtatc cgaggcgggg 60

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

61 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 120

61 ggactgctcg tagtgttttt agctgcgagt tcagtctcca ccgctttggg ctgtcctgat 120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

121 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 180

121 atttctatgg tccgcaaagg gggaccttcg agggagcacg cgagaggaca aggctgggac 180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

181 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 240

181 ggcgaatggc ctatggacag gcggaaagag ggaagccctt cgcaccgcga aagagtatcg 240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

241 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 300

241 agtgcgacat ccatagagtc aagccacatc cagcaagcga ggttcgaccc gacacacgtg 300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

301 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 360

301 cttggggggc aagtcgggct ggcgacgcgg aataggccat tgatagcaga actcaggttg 360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

361 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 420

361 ggccattctg tgctgaatag cggtgaccgt cgtcggtgac cattgtccta atcgtctcgc 420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

421 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 480

421 tccatacatc cgccacgatg tctcaagaac ttcaccaccg gattgatgcc gatgtgatct 480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

481 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 540

481 tcctgtcata aaccatagac gcgagacgac ttcggtcaat ggaagccttt ttctcaacca 540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

541 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 600

541 tcgagaacta ggccgtttgt ttggtggcga ccatcgccac caaaaaaaca aacgttcgtc 600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

601 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 660

601 gtctaatgcg cgtctttttt tcctagagtt cttctaggaa actagaaaag atgccccaga 660

.......... ........ pBR322_origin ........ .......... ..........

.......... NotI(674) .......... .......... .......... ..........

661 gacgctcagt gggcggccgc atttgcatgt cgctatgtgt tctgggaaat caccataaac 720

661 ctgcgagtca cccgccggcg taaacgtaca gcgatacaca agacccttta gtggtatttg 720

.......... .......... .......... H1_primer .......... ..........

.......... .......... .......... BamHI(755) .......... ..........

721 gtgaaatgtc tttggatttg ggaatcttat aagtggatcc tgagaccgtc tcaaaaattt 780

721 cactttacag aaacctaaac ccttagaata ttcacctagg actctggcag agtttttaaa 780

.......... .......... .......... .......... .......... ..........

NheI(788) .......... .......... .......... .......... ..........

781 ttgagacgct agccacaaga tatataaagc caagaaatcg aaatactttc aagttacggt 840

781 aactctgcga tcggtgttct atatatttcg gttctttagc tttatgaaag ttcaatgcca 840

.......... .......... ......... hU6_promoter ......... ..........

NdeI(845) .......... .......... .......... .......... ..........

841 aagcatatga tagtccattt taaaacataa ttttaaaact gcaaactacc caagaaatta 900

841 ttcgtatact atcaggtaaa attttgtatt aaaattttga cgtttgatgg gttctttaat 900

LKO1_5_primer ..... hU6_promoter ......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ttactttcta cgtcacgtat tttgtactaa tatctttgtg tttacagtca aattaattct 960

901 aatgaaagat gcagtgcata aaacatgatt atagaaacac aaatgtcagt ttaattaaga 960

.......... ......... hU6_promoter ......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 aattatctct ctaacagcct tgtatcgtat atgcaaatat gaaggaatca tgggaaatag 1020

961 ttaatagaga gattgtcgga acatagcata tacgtttata cttccttagt accctttatc 1020

.......... ......... hU6_promoter ......... .......... ..........

.......... .......... .......... .......... .......... PmlI(1075)

1021 gccctcttcc tgcccgacct tggcgcgcgc tcggcgcgcg gtcacgctcc gtcacgtggt 1080

1021 cgggagaagg acgggctgga accgcgcgcg agccgcgcgc cagtgcgagg cagtgcacca 1080

......... hU6_promoter ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gcgttttgcc tgcgcgtctt tccactgggg acgcgtggtg tggaaagtcc ccaggctccc 1140

1081 cgcaaaacgg acgcgcagaa aggtgacccc tgcgcaccac acctttcagg ggtccgaggg 1140

.......... .......... .......... . SV40_enhancer SV40_promoter .

.......... .......... .......... .......... .......... ..........

1141 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt 1200

1141 gtcgtccgtc ttcatacgtt tcgtacgtag agttaatcag tcgttggtcc acacctttca 1200

.......... . SV40_enhancer SV40_promoter . .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 1260

1201 ggggtccgag gggtcgtccg tcttcatacg tttcgtacgt agagttaatc agtcgttggt 1260

.......... . SV40_enhancer SV40_promoter . .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 1320

1261 atcagggcgg ggattgaggc gggtagggcg gggattgagg cgggtcaagg cgggtaagag 1320

.......... . SV40_enhancer SV40_promoter SV40_origin .........

.......... .......... .......... .......... .......... ..........

1321 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 1380

1321 gcggggtacc gactgattaa aaaaaataaa tacgtctccg gctccggcgg agccggagac 1380

......... SV40_promoter SV40_origin SV40pro_F_primer .........

.......... .......... .......... StuI(1417) .......... ..........

1381 agctattcca gaagtagtga ggaggctttt ttggaggcct agtctcgatg ttgacaatta 1440

1381 tcgataaggt cttcatcact cctccgaaaa aacctccgga tcagagctac aactgttaat 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... SalI(1489) MscI(1499)

1441 atcatcggca tagtatatcg gcatagtata atacgacgga tcaaggaggt cgaccatggc 1500

1441 tagtagccgt atcatatagc cgtatcatat tatgctgcct agttcctcca gctggtaccg 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... AatII(1542) .........

1501 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt 1560

1501 gttcaactgg tcacggcaag gccacgagtg gcgcgcgctg cagcggcctc gccagctcaa 1560

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... SmaI(1586) .......... .......... ..........

1561 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt 1620

1561 gacctggctg gccgagccca agagggccct gaagcacctc ctgctgaagc ggccacacca 1620

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac 1680

1621 ggccctgctg cactgggaca agtagtcgcg ccaggtcctg gtccaccacg gcctgttgtg 1680

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt 1740

1681 ggaccggacc cacacccacg cgccggacct gctcgacatg cggctcacca gcctccagca 1740

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg 1800

1741 caggtgcttg aaggccctgc ggaggcccgg ccggtactgg ctctagccgc tcgtcggcac 1800

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga 1860

1801 ccccgccctc aagcgggacg cgctgggccg gccgttgacg cacgtgaagc accggctcct 1860

.......... ......... bleo sh_ble ......... .......... ..........

......... EcoRV(1879) ......... .......... .......... ..........

1861 gcaggactga actagcgata tcactagggg gaggctaact gaaacacgga aggagacaat 1920

1861 cgtcctgact tgatcgctat agtgatcccc ctccgattga ctttgtgcct tcctctgtta 1920

sh_ble .......... .......... ....... TK_PA_terminator .......

.......... .......... .......... .......... .......... ..........

1921 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg 1980

1921 tggccttcct tgggcgcgat actgccgtta tttttctgtc ttattttgcg tgccacaacc 1980

.......... ....... TK_PA_terminator ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc 2040

1981 cagcaaacaa gtatttgcgc cccaagccag ggtcccgacc gtgagacagc tatggggtgg 2040

.......... ....... TK_PA_terminator ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag 2100

2041 ctctggggta accccggtta tgcgggcgca aagaaggaaa aggggtgggg tggggggttc 2100

.......... ....... TK_PA_terminator ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcagg..... .......... 2145

2101 aagcccactt ccgggtcccg agcgtcggtt gcagccccgc cgtcc..... .......... 2145

....... TK_PA_terminator ....... .......... .......... ..........