Relevant mutations/deletions: Splice for 3' Tags
Fusion proteins or tags: ECFP
Terminal: C terminal on backbone
Vector backbone: NA
Type of vector: Mammalian expression,Cre/Lox,Acceptor vector
Backbone size (bp): 4879
5' Sequencing primer: CMV-F
Bacteria resistance: Kanamycin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Plasmid Provided In: DH5a
Principal Investigator: Tony Pawson
Comments: Acceptor vector for the Creator Splice system.
Article: Modification of the Creator recombination system for proteomics applications--improved expression by addition of splice sites. Colwill K et al. (BMC Biotechnol. 2006 . 6():13. Pubmed)