Sequence of V956

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Sequence of V956_pLPS

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Sequence of V956 pLPS-3'ECFP - Acceptor : 4879 BP; 1192 A; 1304 C; 1259 G; 1124 T; 0 other;

AseI(7) .......... .......... .......... .......... ..........

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 60

1 atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat acctcaaggc 60

.......... .......... .... CMV_immearly_promoter .... ..........

.......... .......... .......... .......... .......... ..........

61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc < font color="#000000" style="font-size:10px" face="Courier">cccgcccatt 120

61 gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg gggcgggtaa 120

.......... ... CMV_immearly_promoter CAG_enhancer ... ..........

.......... .......... .......... .......... .......... ..........

121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 180

121 ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg taactgcagt 180

........ CAG_enhancer CMV_immearly_promoter ........ ..........

.......... .......... .......... .......... .......... NdeI(234)

181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 240

181 tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca tagtatacgg 240

........ CAG_enhancer CMV_immearly_promoter ........ ..........

.......... .......... .......... .......... .......... ..........

241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 300

241 ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa tacgggtcat 300

........ CAG_enhancer CMV_immearly_promoter ........ ..........

.......... .......... .......... .......... .......... ..........

301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 360

301 gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt agcgataatg 360

........ CAG_enhancer CMV_immearly_promoter ........ ..........

.......... .......... .......... .......... .......... ..........

361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 420

361 gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac tgagtgcccc 420

.......... .... CMV_immearly_promoter .... .......... ..........

.......... .......... .......... .......... .......... ..........

421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 480

421 taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg ttttagttgc 480

.......... .... CMV_immearly_promoter .... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 540

481 cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc catccgcaca 540

.......... .... CMV_immearly_promoter . CMV_fwd_primer CMV_promoter

.......... .......... .......... .......... .......... NheI(591)

541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcataa 600

541 tgccaccctc cagatatatt cgtctcgacc aaatcacttg gcagtctagg cgatcgtatt 600

... CMV_immearly_promoter CMV_promoter ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 cttcgtatag catacattat acgaagttat agatccaata ttattgaagc atttatcagg 660

601 gaagcatatc gtatgtaata tgcttcaata tctaggttat aataacttcg taaatagtcc 660

.......... loxP .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 720

661 caataacaga gtactcgcct atgtataaac ttacataaat ctttttattt gtttatcccc 720

........ AmpR_promoter ........ .......... .......... ..........

.......... .......... ........ XhoI(762) SacI(769),HindIII(771) EcoRI(778)

721 ttccgcgcac atttccccga aaagtgccac ctgacgtgga tctcgagctc aagcttcgaa 780

721 aaggcgcgtg taaaggggct tttcacggtg gactgcacct agagctcgag ttcgaagctt 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... AgeI(836)

781 ttcagggttt ccttgacaat atcatactta tcctgtccct tttttttcca cagctaccgg 840

781 aagtcccaaa ggaactgtta tagtatgaat aggacaggga aaaaaaaggt gtcgatggcc 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg 900

841 actcgttccc gctcctcgac aagtggcccc accacgggta ggaccagctc gacctgccgc 900

.......... .......... ........ ECFP EGFP_N_primer ........

.......... .......... .......... .......... .......... ..........

901 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca 960

901 tgcatttgcc ggtgttcaag tcgcacaggc cgctcccgct cccgctacgg tggatgccgt 960

.......... .......... ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg 1020

961 tcgactggga cttcaagtag acgtggtggc cgttcgacgg gcacgggacc gggtgggagc 1020

.......... .......... ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tgaccaccct gacctggggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc 1080

1021 actggtggga ctggaccccg cacgtcacga agtcggcgat ggggctggtg tacttcgtcg 1080

.......... .......... ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca 1140

1081 tgctgaagaa gttcaggcgg tacgggcttc cgatgcaggt cctcgcgtgg tagaagaagt 1140

.......... .......... ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga 1200

1141 tcctgctgcc gttgatgttc tgggcgcggc tccacttcaa gctcccgctg tgggaccact 1200

.......... .......... ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc 1260

1201 tggcgtagct cgacttcccg tagctgaagt tcctcctgcc gttgtaggac cccgtgttcg 1260

.......... .......... ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tggagtacaa ctacatcagc cacaacgtct atatcaccgc cgacaagcag aagaacggca 1320

1261 acctcatgtt gatgtagtcg gtgttgcaga tatagtggcg gctgttcgtc ttcttgccgt 1320

.......... .......... ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc 1380

1321 agttccggtt gaagttctag gcggtgttgt agctcctgcc gtcgcacgtc gagcggctgg 1380

.......... .......... ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc 1440

1381 tgatggtcgt cttgtggggg tagccgctgc cggggcacga cgacgggctg ttggtgatgg 1440

.......... .......... ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc 1500

1441 actcgtgggt caggcgggac tcgtttctgg ggttgctctt cgcgctagtg taccaggacg 1500

.......... .......... ECFP .......... ........ EGFP_C_primer

.......... .......... .......... .......... .......... XbaI(1557)

1501 tggagttcgt gaccgccgcc gggatcactc tcggcgtaga cgagctgtac aagtaatcta 1560

1501 acctcaagca ctggcggcgg ccctagtgag agccgcatct gctcgacatg ttcattagat 1560

EGFP_C_primer ...... ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca 1620

1561 ctagtattag tcggtatggt gtaaacatct ccaaaatgaa cgaaattttt tggagggtgt 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... HpaI(1666) ..........

1621 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc 1680

1621 ggagggggac ttggactttg tattttactt acgttaacaa caacaattga acaaataacg 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 1740

1681 tcgaatatta ccaatgttta tttcgttatc gtagtgttta aagtgtttat ttcgtaaaaa 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... AflII(1785) .........

1741 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg 1800

1741 aagtgacgta agatcaacac caaacaggtt tgagtagtta catagaattc cgcatttaac 1800

.......... ........ EBV_rev_primer ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta 1860

1801 attcgcaatt ataaaacaat tttaagcgca atttaaaaac aatttagtcg agtaaaaaat 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt 1920

1861 tggttatccg gctttagccg ttttagggaa tatttagttt tcttatctgg ctctatccca 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca 1980

1921 actcacaaca aggtcaaacc ttgttctcag gtgataattt cttgcacctg aggttgcagt 1980

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

1981 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa 2040

1981 ttcccgcttt ttggcagata gtcccgctac cgggtgatgc acttggtagt gggattagtt 2040

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat 2100

2041 caaaaaaccc cagctccacg gcatttcgtg atttagcctt gggatttccc tcgggggcta 2100

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag 2160

2101 aatctcgaac tgcccctttc ggccgcttgc accgctcttt ccttcccttc tttcgctttc 2160

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg 2220

2161 ctcgcccgcg atcccgcgac cgttcacatc gccagtgcga cgcgcattgg tggtgtgggc 2220

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg 2280

2221 ggcgcgaatt acgcggcgat gtcccgcgca gtccaccgtg aaaagcccct ttacacgcgc 2280

f1_origin .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 2340

2281 cttggggata aacaaataaa aagatttatg taagtttata cataggcgag tactctgtta 2340

.......... .......... .......... ........ AmpR_promoter ........

.......... .......... .......... .......... .......... ..........

2341 aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca 2400

2341 ttgggactat ttacgaagtt attataactt tttccttctc aggactccgc ctttcttggt 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag 2460

2401 cgacacctta cacacagtca atcccacacc tttcaggggt ccgaggggtc gtccgtcttc 2460

........ pBABE_3_primer .... SV40_enhancer SV40_promoter ......

.......... .......... .......... .......... .......... ..........

2461 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 2520

2461 atacgtttcg tacgtagagt taatcagtcg ttggtccaca cctttcaggg gtccgagggg 2520

.......... . SV40_enhancer SV40_promoter . .......... ..........

.......... .......... .......... .......... .......... ..........

2521 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 2580

2521 tcgtccgtct tcatacgttt cgtacgtaga gttaatcagt cgttggtatc agggcgggga 2580

.......... . SV40_enhancer SV40_promoter . .......... ..........

.......... .......... .......... .......... .......... ..........

2581 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 2640

2581 ttgaggcggg tagggcgggg attgaggcgg gtcaaggcgg gtaagaggcg gggtaccgac 2640

.......... SV40_enhancer SV40_promoter SV40_origin ..........

.......... .......... .......... .......... .......... ..........

2641 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa 2700

2641 tgattaaaaa aaataaatac gtctccggct ccggcggagc cggagactcg ataaggtctt 2700

SV40_origin SV40_promoter SV40pro_F_primer ........ ..........

.......... .......... .......... .......... .......... ..........

2701 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat 2760

2701 catcactcct ccgaaaaaac ctccggatcc gaaaacgttt ctagctagtt ctctgtccta 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... EagI(2809) ..........

2761 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg 2820

2761 ctcctagcaa agcgtactaa cttgttctac ctaacgtgcg tccaagaggc cggcgaaccc 2820

.......... .......... ..... Orf frame 3 Neomycin ..... ..........

.......... .......... .......... .......... .......... ..........

2821 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg 2880

2821 acctctccga taagccgata ctgacccgtg ttgtctgtta gccgacgaga ctacggcggc 2880

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... NarI(2903) .......... .......... ..........

2881 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg 2940

2881 acaaggccga cagtcgcgtc cccgcgggcc aagaaaaaca gttctggctg gacaggccac 2940

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... MscI(2985) ..........

2941 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 3000

2941 gggacttact tgacgttctg ctccgtcgcg ccgatagcac cgaccggtgc tgcccgcaag 3000

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg 3060

3001 gaacgcgtcg acacgagctg caacagtgac ttcgcccttc cctgaccgac gataacccgc 3060

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca 3120

3061 ttcacggccc cgtcctagag gacagtagag tggaacgagg acggctcttt cataggtagt 3120

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc 3180

3121 accgactacg ttacgccgcc gacgtatgcg aactaggccg atggacgggt aagctggtgg 3180

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg 3240

3181 ttcgctttgt agcgtagctc gctcgtgcat gagcctacct tcggccagaa cagctagtcc 3240

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 3300

3241 tactagacct gcttctcgta gtccccgagc gcggtcggct tgacaagcgg tccgagttcc 3300

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata 3360

3301 gctcgtacgg gctgccgctc ctagagcagc actgggtacc gctacggacg aacggcttat 3360

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 3420

3361 agtaccacct tttaccggcg aaaagaccta agtagctgac accggccgac ccacaccgcc 3420

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 3480

3421 tggcgatagt cctgtatcgc aaccgatggg cactataacg acttctcgaa ccgccgctta 3480

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 3540

3481 cccgactggc gaaggagcac gaaatgccat agcggcgagg gctaagcgtc gcgtagcgga 3540

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca 3600

3541 agatagcgga agaactgctc aagaagactc gccctgagac cccaagcttt actggctggt 3600

..... Orf frame 3 Neomycin ..... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt 3660

3601 tcgctgcggg ttggacggta gtgctctaaa gctaaggtgg cggcggaaga tactttccaa 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat 3720

3661 cccgaagcct tagcaaaagg ccctgcggcc gacctactag gaggtcgcgc ccctagagta 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc 3780

3721 cgacctcaag aagcgggtgg gatccccctc cgattgactt tgtgccttcc tctgttatgg 3780

.......... .......... .......... ....... TK_PA_terminator .......

.......... .......... .......... .......... .......... ..........

3781 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc 3840

3781 ccttccttgg gcgcgatact gccgttattt ttctgtctta ttttgcgtgc cacaacccag 3840

.......... ....... TK_PA_terminator ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag 3900

3841 caaacaagta tttgcgcccc aagccagggt cccgaccgtg agacagctat ggggtggctc 3900

.......... ....... TK_PA_terminator ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc 3960

3901 tggggtaacc ccggttatgc gggcgcaaag aaggaaaagg ggtggggtgg ggggttcaag 3960

.......... ....... TK_PA_terminator ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg 4020

3961 cccacttccg ggtcccgagc gtcggttgca gccccgccgt ccgggacggt atcggagtcc 4020

.......... ....... TK_PA_terminator ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 4080

4021 aatgagtata tatgaaatct aactaaattt tgaagtaaaa attaaatttt cctagatcca 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 4140

4081 cttctaggaa aaactattag agtactggtt ttagggaatt gcactcaaaa gcaaggtgac 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 4200

4141 tcgcagtctg gggcatcttt tctagtttcc tagaagaact ctaggaaaaa aagacgcgca 4200

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

4201 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 4260

4201 ttagacgacg aacgtttgtt tttttggtgg cgatggtcgc caccaaacaa acggcctagt 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 4320

4261 tctcgatggt tgagaaaaag gcttccattg accgaagtcg tctcgcgtct atggtttatg 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 4380

4321 acaggaagat cacatcggca tcaatccggt ggtgaagttc ttgagacatc gtggcggatg 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 4440

4381 tatggagcga gacgattagg acaatggtca ccgacgacgg tcaccgctat tcagcacaga 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 4500

4441 atggcccaac ctgagttctg ctatcaatgg cctattccgc gtcgccagcc cgacttgccc 4500

.......... ........ pBR322_origin ........ .......... ..........

ApaLI(4507) ........ .......... .......... .......... ..........

4501 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 4560

4501 cccaagcacg tgtgtcgggt cgaacctcgc ttgctggatg tggcttgact ctatggatgt 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 4620

4561 cgcactcgat actctttcgc ggtgcgaagg gcttccctct ttccgcctgt ccataggcca 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 4680

4621 ttcgccgtcc cagccttgtc ctctcgcgtg ctccctcgaa ggtccccctt tgcggaccat 4680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 4740

4681 agaaatatca ggacagccca aagcggtgga gactgaactc gcagctaaaa acactacgag 4740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 4800

4741 cagtcccccc gcctcggata cctttttgcg gtcgttgcgc cggaaaaatg ccaaggaccg 4800

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 4860

4801 gaaaacgacc ggaaaacgag tgtacaagaa aggacgcaat aggggactaa gacacctatt 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ccgtattacc gccatgcat. .......... .......... .......... .......... 4879

4861 ggcataatgg cggtacgta. .......... .......... .......... .......... 4879

.......... .......... .......... .......... .......... ..........