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Yeast expression Plasmids


pAG303GAL-ccdB to pGAD_GH | pGAD_T7 to pRS408 | pRS410 to yeast_2_micron_A_form | 

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Plasmids 0 - 4Descriptions
small image of pAG303GAL-ccdBpAG303GAL-ccdB - Gene/insert name: None; Vector backbone: pRS303 (Search Vector Database); 5' Sequencing primer: GAL1 (List of Sequencing Primers); Bacteria resistance: Ampicillin,Chloramphenicol; High or low copy: Don't Know; Grow in standard E. coli @ 37C: No; Please specify bacterial strain for growth and growth condition: DB3.1, ccdB Survival; Selectable markers: HIS3; Plasmid Prov...
small image of pAG414GAL-ccdBpAG414GAL-ccdB - Gene/insert name: None ; Vector backbone: pRS414 ; 5' Sequencing primer: GAL1 ; Bacteria resistance: Ampicillin,Chloramphenicol ; High or low copy: Don't Know ; Grow in standard E. coli @ 37C: No; Please specify bacterial strain for growth and growth condition: DB3.1, ccdB Survival ; Selectable markers: TRP1 ; Plasmid Provided In: ccdB Survival ; ...
small image of pAS404pAS404 - Gene/insert name: none; Fusion proteins or tags: HA; Terminal: N terminal on backbone; Vector backbone: pAS404 (Search Vector Database); Cloning site 5': See map; Site destroyed during cloning: No; Cloning site 3': See map; Site destroyed during cloning: No; 5' Sequencing primer: Gal4-Nterm (List of Sequencing Primers); Bacteria resistance: Ampicillin; High or low copy: High Cop...
small image of pBridgepBridge - Vendor:Clontech; Vector Type: Yeast; Constitutive/Inducible: Constitutive; Promoter: ADH1; Sequencing Primer: Match; Sequencing Primer Sequence: 5'd[TCATCGGAAGAGAGTAGT]3'; Bacteria Resistance: Ampicillin; Mammalian Selection: TRP1; Catalog Number: 6184-1; Comments:Used as bait (& bridge) for yeast 3-hybrid screen.
small image of pGAD_GHpGAD GH - Vendor:Clontech; Vector Type: Yeast; Promoter: ADH1; Sequencing Primer: Gal4AD; Sequencing Primer Sequence: 5'd[AATACCACTACAATGGAT]3'; Tag: Gal4-AD (Nterm); Bacteria Resistance: Ampicillin; Mammalian Selection: LEU2; Catalog Number: 6182-1; Comments:Fusion to Gal4-activation domain. Used in yeast 2-hybrid system

Plasmids 5 - 9Descriptions
small image of pGAD_T7pGAD T7 - Vendor:Clontech; Vector Type: Yeast; Promoter: ADH1; Sequencing Primer: T7; Tag: Gal4-AD (Nterm), HA Tag; Bacteria Resistance: Ampicillin; Mammalian Selection: LEU2; Catalog Number: part of MATCHMAKER 2-hybrid; Comments:Fusion to Gal4-activation domain. Used in yeast 2-hybrid system
small image of pGBK_T7pGBK T7 - Vendor:Clontech; Vector Type: Yeast; Promoter: ADH1; Sequencing Primer: T7; Tag: Gal4-DBD(Nterm); Bacteria Resistance: Kanamycin; Mammalian Selection: TRP1; Catalog Number: part of MATCHMAKER 2-hybrid; Comments:Fusion to Gal4-DNA binding domain. Used in yeast 2-hybrid
small image of pHIS-2pHIS-2 - Vendor:Clontech; Vector Type: Yeast; Promoter: none; Bacteria Resistance: Kanamycin; Mammalian Selection: TRP1 (HIS3 selection); Comments:Clone in cis-acting sequences for use with 1-hybrid system
small image of pOBD2pOBD2 - Vendor: Fields lab; Vector Type: yeast expression; Promoter: ADH1 promoter; Sequencing Primer: Gal4_N_term; Sequencing Primer Sequence: GAGTAGTAACAAAGGTCAA; Tag: GAL4-DBD; Bacteria Resistance: ampicillin; ADH1 promoter: 1-298 (at least; region of homology when searched against SGD); GAL4-DBD: 434-982; ADH1 terminator: 1221-1687 (at least; region of homology to SGD genom. seq.); beta lactamas...
small image of pRS408pRS408 - Gene/insert name: none; Vector backbone: pRS408 (Search Vector Database); 5' Sequencing primer: T7 (List of Sequencing Primers); 3' Sequencing primer: M13R; Bacteria resistance: Ampicillin; High or low copy: Low Copy; Grow in standard E. coli @ 37C: Yes; Selectable markers: NatMX; If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive...

Plasmids 10 - 14Descriptions
small image of pRS410pRS410 - Gene/insert name: none; Vector backbone: pRS410 (Search Vector Database); 5' Sequencing primer: T7 (List of Sequencing Primers); 3' Sequencing primer: M13R; Bacteria resistance: Ampicillin; High or low copy: Low Copy; Grow in standard E. coli @ 37C: Yes; Selectable markers: Neomycin; If you did not originally clone this gene, from whom and where did you receive the plasmid used to der...
small image of pRS418pRS418 - Gene/insert name: none ; Vector backbone: pRS418 ; 5' Sequencing primer: T7 ; 3' Sequencing primer: M13R ; Bacteria resistance: Ampicillin ; High or low copy: High Copy ; Grow in standard E. coli @ 37C: Yes ; Selectable markers: NatMX ; If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid: p433...
small image of pRS420pRS420 - Gene/insert name: none; Vector backbone: pRS420 (Search Vector Database); 5' Sequencing primer: T7 (List of Sequencing Primers); 3' Sequencing primer: M13R; Bacteria resistance: Ampicillin; High or low copy: High Copy; Grow in standard E. coli @ 37C: Yes; Selectable markers: Neomycin; If you did not originally clone this gene, from whom and where did you receive the plasmid used to de...
small image of pRS428pRS428 - Gene/insert name: none; Vector backbone: pRS418 (Search Vector Database); 5' Sequencing primer: T7 (List of Sequencing Primers); 3' Sequencing primer: M13R; Bacteria resistance: Ampicillin; High or low copy: High Copy; Grow in standard E. coli @ 37C: Yes; Selectable markers: NatMX; If you did not originally clone this gene, from whom and where did you receive the plasmid used to deriv...
small image of yeast_2_micron_A_formyeast 2 micron A form - GenBank Accession Number: M14593 M14594 M14595 M14596 M14597 M14598; Comments: Yeast 2 micron plasmid contains two 599 bp inverted repeats separated by a large unique (UL) and a small unique (US) region. During recombination the UL and US regions invert producing two sequence; Hosts: S.cerevisiae.; Related vectors: 2 micron. (Information source: VectorDB.)



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