Yeast expression Plasmids
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pRS418
Gene/insert name: none
Insert size (bp): Unknown
Vector backbone: pRS418
Type of vector: Yeast expression
Backbone size (bp): 5043
5' Sequencing primer: T7
3' Sequencing primer: M13R
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Selectable markers: NatMX
If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid: p4339 from Charlie Boone.
Plasmid Provided In: DH5a
Principal Investigator: Fred Cross
Comments: p4339 + pRS410 --> pRS418. Insert is NatMX (clonNAT resistance) from EcoRI-cut p4339. Backbone is NcoI-cut pRS410 (CEN plasmid).
pAG414GAL-ccdB
Gene/insert name: None
Insert size (bp): Unknown
Vector backbone: pRS414
Type of vector: Yeast expression,Gateway Destination
Backbone size (bp): Unknown
5' Sequencing primer: GAL1
Bacteria resistance: Ampicillin,Chloramphenicol
High or low copy: Don't Know
Grow in standard E. coli @ 37C: No
Please specify bacterial strain for growth and growth condition: DB3.1, ccdB Survival
Selectable markers: TRP1
Plasmid Provided In: ccdB Survival
Principal Investigator: Susan Lindquist
Comments: Type of plasmid: CEN.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction
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Copyright 2005 BVTech, Inc. All Rights Reserved
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