^W ^W $1 agatcgcaccactgcactccagcctgggagacagagagagactctatctc 51 aaaaaaaaaagataaaagataaaaaaatactactaataaaacaaaatcag 101 aaagtgtgaaacctccaactttgctcttcttcaagattgtttttgctatt 151 cagctaagacacaactcacttttttttgttgttgttttggagacagggtc 201 tcactcttgcctaggctggagtgaggtggtgcgatctcagctcactgcac 251 tgctgggttcaagtgatccttcaacctcagcctctcaagtagctgggact 301 acaggtgtgtgccaccatgcccagctaattaagtatacaatttcgtgacc 351 atgaagcaacacctatttaacaaccaacctgatcaagaaacacaacactc 401 cccagttccttccctttctcctgcatcctttcctgattacaattccctcc 451 tttccacaggttatcagcagtctgatttttttggtaattattttcttctc 501 tttatagttttaacacttaattatgcatcctcaaacaatataattttacc 551 tatttttcaacttgaatttaaatcatatcatgtaatacttttaacttgca 601 tatattactgaacatcatgaaattcatccatgctgtgtagcagtaattca 651 cacattacatatattatatttctggcttttggtaactatgaacagtactg 701 ctataaattttctttacatgtgttctggtctgcaggtgtaagagtttacc 751 tagaggccatgcgcggtggctcatgcctgtaatcccagcacttcgggagg 801 ccgaggcaggccgatcacttgagtccaggaattcgagactagcctggcca 851 acatggcgaaaccctgtctctactaaaaatacaaaaaattagctaggtgt 901 ggtggtggtgtgcctgtagtctcagctatttgagagggtgaggtaaggag 951 aattgcttgaacctgggaagtggaggttgcaataagccgaaatggcacca 1001 actgcactcaaagtctggacaaaagaacaagaatgttaaaaaaaaaaaac 1051 aaaatttaactaaagtgtatatctaaaaatggaataaatcaatagtagga 1101 tacaaaatgcagaatagaaatgtcaaaccgcttttcagagttgttccaat 1151 ttatacttccaccagtgcacctgggtatcccccaaggatcacttgagccc 1201 aaaagtttgagatcagcctgggcaacatactgagacccccatctgtattt 1251 aaaaaaaaaaaaaattccaacaaataaatgaagagacaatattttgccaa 1301 aaaaggacaaaagtctttaacaagcagttcacacatagctaataaacatt 1351 aaaaagtgtatggcatgactggtaagtaaaccacagagagatatcattac 1401 acacccatcagccggctaaaaattaaatcatgcaatagcaaaagagtatg 1451 tggggccaggaacggtggctcatgcctgcaattgcttaattgcttattat 1501 tattattccattattccacatgctcttttgtttttttgtttgtttattgt 1551 tttttgagatggagtctcactttgtggcccatgctggagtgcagtggcat 1601 gatctcggctcactgcaacctccacctcccaggttcaagaattctcctgc 1651 ctcagtagctgggattacaggtgcgtgccacgacaccaagctaatttttt 1701 tttttttttttttttttttttttgagatggagtctcgctcttttgcccag 1751 gctggagtgcagtggtgtgatcttggctcactacaatctccacctcccag 1801 gttcaagtgattctcctgcctcagcctcccgagtagctggaactacaggc 1851 acctgccacagcatccggctaattttttgggtatttttagtacaggtggg 1901 gtttcagcatgttggccatgctggtctcgaactcctgacctcaaatgatc 1951 cacccgcctcagctgcccaaagtactgggattacaggtgtgagccactgt 2001 tcctggccccacatactcttttgctattgcatgattgtagcgttggcagg 2051 tcctgaggcagctggcaagacgcctgcagctgaaagatacaaggccaggg 2101 acaggacagtcccatccccaggaggcagggagtatacaggctggggaagt 2151 ttgcccttgcgtggggtggtgatggaggaggctcagcaagtcttctggac 2201 tgtgaacctgtgtctgccactgtgtgctgggtggtggtcatctttcccac 2251 caggctgtggcctctgcaaccttcaagggaggagcaggtcccattggctg 2301 agcacagccttgtaccgtgaactggaacaagcagcctccttcctggccac 2351 aggttccatgtccttatatggactcatctttgcctattgcgacacacact 2401 caatgaacacctactacgcgctgcaaagagccccgcaggcctgaggtgcc 2451 cccacctcaccactcttcctatttttgtgtaaaaatccagcttcttgtca 2501 ccacctccaaggagggggaggaggaggaaggcaggttcctctaggctgag 2551 ccgaatgcccctctgtggtcccacgccactgatcgctgcatgcccaccac 2601 ctgggtacacacagtctgtgattcccggagcagaacggaccctgcccacc 2651 cggtcttgtgtgctactcagtggacagacccaaggcaagaaagggtgaca 2701 aggacagggtcttcccaggctggctttgagttcctagcaccgccccgccc 2751 ccaatcctctgtggcacatggagtcttggtccccagagtcccccagcggc 2801 ctccagatggtctgggagggcagttcagctgtggctgcgcatagcagaca 2851 tacaacggacggtgggcccagacccaggctgtgtagacccagcccccccg 2901 ccccgcagtgcctaggtcacccactaacgccccaggcctggtcttggctg 2951 ggcgtgactgttaccctcaaaagcaggcagctccagggtaaaaggtgccc 3001 tgccctgtagagcccaccttccttcccagggctgcggctgggtaggtttg 3051 tagccttcatcacgggccacctccagccactggaccgctggcccctgccc 3101 tgtcctggggagtgtggtcctgcgacttctaagtggccgcaagccacctg 3151 actcccccaacaccacactctacctctcaagcccaggtctctccctagtg 3201 acccacccagcacatttagctagctgagccccacagccagaggtcctcag 3251 gccctgctttcagggcagttgctctgaagtcggcaagggggagtgactgc 3301 ctggccactccatgccctccaagagctccttctgcaggagcgtacagaac 3351 ccagggccctggcacccgtgcagaccctggcccaccccacctgggcgctc 3401 agtgcccaagagatgtccacacctaggatgtcccgcggtgggtggggggc 3451 ccgagagacgggcaggccgggggcaggcctggccatgcggggccgaaccg 3501 ggcactgcccagcgtggggcgcgggggccacggcgcgcgcccccagcccc 3551 cgggcccagcaccccaaggcggccaacgccaaaactctccctcctcctct 3601 tcctcaatctcgctctcgctctttttttttttcgcaaaaggaggggagag 3651 ggggtaaaaaaatgctgcactgtgcggcgaagccggtgagtgagcggcgc 3701 ggggccaatcagcgtgcgccgttccgaaagttgccttttatggctcgagc 3751 ggccgcggcggcgccctataaaacccagcggcgcgacgcgccaccaccgc 3801 cgagaccgcgtccgccccgcgagcacagagcctcgcctttgccgatccgc 3851 cgcccgtccacacccgccgccaggtaagcccggccagccgaccggggcat 3901 gcggccgcggccccttcgcccgtgcagagccgccgtctgggccgcagcgg 3951 ggggcgcatggggggggaaccggaccgccgtggggggcgcgggagaagcc 4001 cctgggcctccggagatgggggacaccccacgccagttcggaggcgcgag 4051 gccgcgctcgggaggcgcgctccgggggtgccgctctcggggcgggggca 4101 accggcggggtctttgtctgagccgggctcttgccaatggggatcgcagg 4151 gtgggcgcggcgaagcccccgccaggcccggtgggggctggggcgccatt 4201 gcgcgtgcgcgctggtcctttgggcgctaactgcgtgcgcgctgggaatt 4251 ggcgctaattgcgcgtgcgcgctgggactcaaggcgctaaatgcgcgtgc 4301 gttctggggcccggggtgccgcggcctgggctggggcgaaggcgggctcg 4351 gccggaaggggtggggtcgccgcggctcccgggcgcttgcgcgcacttcc 4401 tgcccgagccgctggccgcccgagggtgtggccgctgcgtgcgcgcgcgc 4451 cgacccggcgctgtttgaaccgggcggaggcggggctggcgcccggttgg 4501 gagggggttggggcctggcttcctgccgcgcgccgcggggacgcctccga 4551 ccagtgtttgccttttatggtaataacgcggccggcccggcttcctttgt 4601 ccccaatctgggcgcgcgccggcgccccctggcggcctaaggactcggcg 4651 cgccggaagtggccagggcgggggcgacctcggctcacagcgcgcccggc 4701 tattctcgcagctcacatcgaaggtaaacgaattcccccaccatggtgag 4751 caagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctgg 4801 acggcgacgtaaacggccacaagttcagcgtgtctggcgagggcgagggc 4851 gatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaa 4901 gctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgc 4951 agtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaag 5001 tccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaagga 5051 cgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccc 5101 tggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaac 5151 atcctggggcacaagctggagtacaactacaacagccacaacgtctatat 5201 catggccgacaagcagaagaacggcatcaaggcgaacttcaagatccgcc 5251 acaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaac 5301 acccccatcggcgacggccccgtgctgctgcccgacaaccactacctgag 5351 cacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatgg 5401 tcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgag 5451 ctgtacaagtaatgaattaattaagaattatcaccgcttctattcagcca 5501 gtaaggcctgtctttaatggcctccggcatgagacactttctagaattcg 5551 gtacccccccccccaacgttactggccgaagccgcttggaataaggccgg 5601 tgtgcgtttgtctatatgttattttccaccatattgccgtcttttggcaa 5651 tgtgagggcccggaaacctggccctgtcttcttgacgagcattcctaggg 5701 gtctttcccctctcgccaaaggaatgcaaggtctgttgaatgtcgtgaag 5751 gaagcagttcctctggaagcttcttgaagacaaacaacgtctgtagcgac 5801 cctttgcaggcagcggaaccccccacctggcgacaggtgcctctgcggcc 5851 aaaagccacgtgtataagatacacctgcaaaggcggcacaaccccagtgc 5901 cacgttgtgagttggatagttgtggaaagagtcaaatggctctcctcaag 5951 cgtattcaacaaggggctgaaggatgcccagaaggtaccccattgtatgg 6001 gatctgatctggggcctcggtgcacatgctttacatgtgtttagtcgagg 6051 ttaaaaaaacgtctaggccccccgaaccacggggacgtggtttcctaatt 6101 tttagcaggctgatgggtgtgtaatgatatctctctgtggttaattacca 6151 gtcatgccatacgctttttaatgtttattagctatgtgtgaactgcttgt 6201 taaagacttttgcccattgttggcaaaaggttgtctcttcgtttatttgt 6251 tggaattttttgtttgtttgtttgtttttaaatacaggtgggggtctcag 6301 tatgttacccaggctggtctcaaacttctgggctcaagtgatcctcctgc 6351 ctctgccttctcaaagaactgggattacaggcatgagccattatgcccag 6401 cctattggtagaagttctttatatgttttgaatatttatcgcacatttag 6451 gcatccagttttgtgaaagccctcagttttggatcacttttatattagtt 6501 ttttttttagatcagtttgtagaagttctttatatatttttgatatgact 6551 gctttatcagacccatgtacagcaaatattttctcccactctttggtttg 6601 cctttccactctttcggtgatgtcttttggtgaacagaagtttttaattt 6651 tactacagtcccatgtatgaattgttttaattatgattactgcctttcaa 6701 aaacatttaagaaagttttacctactcaaagtttatgaaaatattcttct 6751 cctatgctctcttctaaaatatgtattgctttacctttccaattttaagg 6801 ataatacaactgaaactaactttttttaaaagtaacttttaggctgggcg 6851 tggtggctcacgcctgtaatcccagtacttcaggaggccgacattgggga 6901 tatcacctgaggtcaggagttcgagaccagcctggccaacatggtgaaac 6951 tccatatctactaaaaacaaaacttagccaggtgtggtggcagacacctg 7001 taatcccagctactcgggaggctgaggcaggagaatcacttgaacccagg 7051 aggcggaggttgcagtgagccgagattatgccattgcactccagcctggg 7101 tgacagagcaagactccatctcaaaaagaaataaataaataaaaaataaa 7151 agtaacttttaaatgattgtgaggtagggatcaagtttaatgtttttcca 7201 tagggatattcaattggcccatcccaaaaagaccggcctttctccactac 7251 acagcagtgttgactttgtcatctatcaggtgacccttcagcctgtctgt 7301 ggactctttgttgtgttccattggcctgtttgtctattgttggaacaaaa 7351 ccacactgtctgaattgccacagctttataatagggcatgctatcaagca 7401 gtcagtgtcattttacaggtttgttcttctttaggtttgccttagctatt 7451 ctttggtctttgcattgccatctaaattttagaatcaactaagtcaaaat 7501 gttgtatcactttccacaccaaaaatctgctggaattttgattgggatta 7551 tattaaatttgtagatcaatttgggaagaaataagatctttacaacatta 7601 actcttccaatctataaatgtggtatttattttttctttatttttttctc 7651 agtggtttgtagtttctagtgcaaaggtctttgacatcgttagatttaat 7701 gttttttgatatattttggtgttataagtgatacttttcaaaatttcatt 7751 ttctatttgtttcttgttgacacagcaacacaactggttttagtgtaata 7801 atcttatatttagagcccttgctaattaaacctattaattataatagttc 7851 ttctatagattctttcagcttttttgcatatacgatcatgtcatctgtga 7901 gtaatgacatgaacccaggaggcgttctttccaggctttctttttatagt 7951 catgtactgcttaacgacgtttttgtcgatgatgaactgcatatacaaca 8001 gtggtcccataagattataaagaggctgaaaaattcttactgcctagtga 8051 cactgtagccatcataatgtagcacaattcagtac Beta-actin GFP "Times New Roman^W C EGFP_N_primer"Times New Roman<^W CSEGFPTimes New Roman^W CɆV Orf frame 3"Times New Roman^W ) EGFP_C_primer"Times New Roman<^W CIRES"Times New Roman ^W BclI"Times New Roman| fS?0? ^W FspI"Times New Roman  ,=MM ^W NheI"Times New Roman  X ^W SacI"Times New Roman  ^W XhoI"Times New Roman,,; ^W NcoI"Times New Roman ^W XbaI"Times New Roman U ^W HindIII"Times New Romanimq:jx ^W PmlI"Times New RomanZ]`FYg ^W BglII"Times New Roman *o#\I:;I