Sequence of beta-actinGFP

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Sequence of beta-actinGFP

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Sequence of Beta-actin GFP : 8085 BP; 1877 A; 2134 C; 2028 G; 2046 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 agatcgcacc actgcactcc agcctgggag acagagagag actctatctc aaaaaaaaaa 60

1 tctagcgtgg tgacgtgagg tcggaccctc tgtctctctc tgagatagag tttttttttt 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 gataaaagat aaaaaaatac tactaataaa acaaaatcag aaagtgtgaa acctccaact 120

61 ctattttcta tttttttatg atgattattt tgttttagtc tttcacactt tggaggttga 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ttgctcttct tcaagattgt ttttgctatt cagctaagac acaactcact tttttttgtt 180

121 aacgagaaga agttctaaca aaaacgataa gtcgattctg tgttgagtga aaaaaaacaa 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gttgttttgg agacagggtc tcactcttgc ctaggctgga gtgaggtggt gcgatctcag 240

181 caacaaaacc tctgtcccag agtgagaacg gatccgacct cactccacca cgctagagtc 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ctcactgcac tgctgggttc aagtgatcct tcaacctcag cctctcaagt agctgggact 300

241 gagtgacgtg acgacccaag ttcactagga agttggagtc ggagagttca tcgaccctga 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 acaggtgtgt gccaccatgc ccagctaatt aagtatacaa tttcgtgacc atgaagcaac 360

301 tgtccacaca cggtggtacg ggtcgattaa ttcatatgtt aaagcactgg tacttcgttg 360

.......... .......... .......... .......... .......... ..........

.......... BclI(380) .......... .......... .......... ..........

361 acctatttaa caaccaacct gatcaagaaa cacaacactc cccagttcct tccctttctc 420

361 tggataaatt gttggttgga ctagttcttt gtgttgtgag gggtcaagga agggaaagag 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ctgcatcctt tcctgattac aattccctcc tttccacagg ttatcagcag tctgattttt 480

421 gacgtaggaa aggactaatg ttaagggagg aaaggtgtcc aatagtcgtc agactaaaaa 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ttggtaatta ttttcttctc tttatagttt taacacttaa ttatgcatcc tcaaacaata 540

481 aaccattaat aaaagaagag aaatatcaaa attgtgaatt aatacgtagg agtttgttat 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 taattttacc tatttttcaa cttgaattta aatcatatca tgtaatactt ttaacttgca 600

541 attaaaatgg ataaaaagtt gaacttaaat ttagtatagt acattatgaa aattgaacgt 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tatattactg aacatcatga aattcatcca tgctgtgtag cagtaattca cacattacat 660

601 atataatgac ttgtagtact ttaagtaggt acgacacatc gtcattaagt gtgtaatgta 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 atattatatt tctggctttt ggtaactatg aacagtactg ctataaattt tctttacatg 720

661 tataatataa agaccgaaaa ccattgatac ttgtcatgac gatatttaaa agaaatgtac 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tgttctggtc tgcaggtgta agagtttacc tagaggccat gcgcggtggc tcatgcctgt 780

721 acaagaccag acgtccacat tctcaaatgg atctccggta cgcgccaccg agtacggaca 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 aatcccagca cttcgggagg ccgaggcagg ccgatcactt gagtccagga attcgagact 840

781 ttagggtcgt gaagccctcc ggctccgtcc ggctagtgaa ctcaggtcct taagctctga 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 agcctggcca acatggcgaa accctgtctc tactaaaaat acaaaaaatt agctaggtgt 900

841 tcggaccggt tgtaccgctt tgggacagag atgattttta tgttttttaa tcgatccaca 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ggtggtggtg tgcctgtagt ctcagctatt tgagagggtg aggtaaggag aattgcttga 960

901 ccaccaccac acggacatca gagtcgataa actctcccac tccattcctc ttaacgaact 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 acctgggaag tggaggttgc aataagccga aatggcacca actgcactca aagtctggac 1020

961 tggacccttc acctccaacg ttattcggct ttaccgtggt tgacgtgagt ttcagacctg 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 aaaagaacaa gaatgttaaa aaaaaaaaac aaaatttaac taaagtgtat atctaaaaat 1080

1021 ttttcttgtt cttacaattt tttttttttg ttttaaattg atttcacata tagattttta 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ggaataaatc aatagtagga tacaaaatgc agaatagaaa tgtcaaaccg cttttcagag 1140

1081 ccttatttag ttatcatcct atgttttacg tcttatcttt acagtttggc gaaaagtctc 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ttgttccaat ttatacttcc accagtgcac ctgggtatcc cccaaggatc acttgagccc 1200

1141 aacaaggtta aatatgaagg tggtcacgtg gacccatagg gggttcctag tgaactcggg 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 aaaagtttga gatcagcctg ggcaacatac tgagaccccc atctgtattt aaaaaaaaaa 1260

1201 ttttcaaact ctagtcggac ccgttgtatg actctggggg tagacataaa tttttttttt 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 aaaattccaa caaataaatg aagagacaat attttgccaa aaaaggacaa aagtctttaa 1320

1261 ttttaaggtt gtttatttac ttctctgtta taaaacggtt ttttcctgtt ttcagaaatt 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 caagcagttc acacatagct aataaacatt aaaaagtgta tggcatgact ggtaagtaaa 1380

1321 gttcgtcaag tgtgtatcga ttatttgtaa tttttcacat accgtactga ccattcattt 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ccacagagag atatcattac acacccatca gccggctaaa aattaaatca tgcaatagca 1440

1381 ggtgtctctc tatagtaatg tgtgggtagt cggccgattt ttaatttagt acgttatcgt 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aaagagtatg tggggccagg aacggtggct catgcctgca attgcttaat tgcttattat 1500

1441 tttctcatac accccggtcc ttgccaccga gtacggacgt taacgaatta acgaataata 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tattattcca ttattccaca tgctcttttg tttttttgtt tgtttattgt tttttgagat 1560

1501 ataataaggt aataaggtgt acgagaaaac aaaaaaacaa acaaataaca aaaaactcta 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ggagtctcac tttgtggccc atgctggagt gcagtggcat gatctcggct cactgcaacc 1620

1561 cctcagagtg aaacaccggg tacgacctca cgtcaccgta ctagagccga gtgacgttgg 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tccacctccc aggttcaaga attctcctgc ctcagtagct gggattacag gtgcgtgcca 1680

1621 aggtggaggg tccaagttct taagaggacg gagtcatcga ccctaatgtc cacgcacggt 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cgacaccaag ctaatttttt tttttttttt tttttttttt tttgagatgg agtctcgctc 1740

1681 gctgtggttc gattaaaaaa aaaaaaaaaa aaaaaaaaaa aaactctacc tcagagcgag 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ttttgcccag gctggagtgc agtggtgtga tcttggctca ctacaatctc cacctcccag 1800

1741 aaaacgggtc cgacctcacg tcaccacact agaaccgagt gatgttagag gtggagggtc 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gttcaagtga ttctcctgcc tcagcctccc gagtagctgg aactacaggc acctgccaca 1860

1801 caagttcact aagaggacgg agtcggaggg ctcatcgacc ttgatgtccg tggacggtgt 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gcatccggct aattttttgg gtatttttag tacaggtggg gtttcagcat gttggccatg 1920

1861 cgtaggccga ttaaaaaacc cataaaaatc atgtccaccc caaagtcgta caaccggtac 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ctggtctcga actcctgacc tcaaatgatc cacccgcctc agctgcccaa agtactggga 1980

1921 gaccagagct tgaggactgg agtttactag gtgggcggag tcgacgggtt tcatgaccct 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ttacaggtgt gagccactgt tcctggcccc acatactctt ttgctattgc atgattgtag 2040

1981 aatgtccaca ctcggtgaca aggaccgggg tgtatgagaa aacgataacg tactaacatc 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cgttggcagg tcctgaggca gctggcaaga cgcctgcagc tgaaagatac aaggccaggg 2100

2041 gcaaccgtcc aggactccgt cgaccgttct gcggacgtcg actttctatg ttccggtccc 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 acaggacagt cccatcccca ggaggcaggg agtatacagg ctggggaagt ttgcccttgc 2160

2101 tgtcctgtca gggtaggggt cctccgtccc tcatatgtcc gaccccttca aacgggaacg 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gtggggtggt gatggaggag gctcagcaag tcttctggac tgtgaacctg tgtctgccac 2220

2161 caccccacca ctacctcctc cgagtcgttc agaagacctg acacttggac acagacggtg 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tgtgtgctgg gtggtggtca tctttcccac caggctgtgg cctctgcaac cttcaaggga 2280

2221 acacacgacc caccaccagt agaaagggtg gtccgacacc ggagacgttg gaagttccct 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ggagcaggtc ccattggctg agcacagcct tgtaccgtga actggaacaa gcagcctcct 2340

2281 cctcgtccag ggtaaccgac tcgtgtcgga acatggcact tgaccttgtt cgtcggagga 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tcctggccac aggttccatg tccttatatg gactcatctt tgcctattgc gacacacact 2400

2341 aggaccggtg tccaaggtac aggaatatac ctgagtagaa acggataacg ctgtgtgtga 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 caatgaacac ctactacgcg ctgcaaagag ccccgcaggc ctgaggtgcc cccacctcac 2460

2401 gttacttgtg gatgatgcgc gacgtttctc ggggcgtccg gactccacgg gggtggagtg 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cactcttcct atttttgtgt aaaaatccag cttcttgtca ccacctccaa ggagggggag 2520

2461 gtgagaagga taaaaacaca tttttaggtc gaagaacagt ggtggaggtt cctccccctc 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gaggaggaag gcaggttcct ctaggctgag ccgaatgccc ctctgtggtc ccacgccact 2580

2521 ctcctccttc cgtccaagga gatccgactc ggcttacggg gagacaccag ggtgcggtga 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gatcgctgca tgcccaccac ctgggtacac acagtctgtg attcccggag cagaacggac 2640

2581 ctagcgacgt acgggtggtg gacccatgtg tgtcagacac taagggcctc gtcttgcctg 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cctgcccacc cggtcttgtg tgctactcag tggacagacc caaggcaaga aagggtgaca 2700

2641 ggacgggtgg gccagaacac acgatgagtc acctgtctgg gttccgttct ttcccactgt 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 aggacagggt cttcccaggc tggctttgag ttcctagcac cgccccgccc ccaatcctct 2760

2701 tcctgtccca gaagggtccg accgaaactc aaggatcgtg gcggggcggg ggttaggaga 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gtggcacatg gagtcttggt ccccagagtc ccccagcggc ctccagatgg tctgggaggg 2820

2761 caccgtgtac ctcagaacca ggggtctcag ggggtcgccg gaggtctacc agaccctccc 2820

.......... .......... .......... .......... .......... ..........

.......... FspI(2838) .......... .......... .......... ..........

2821 cagttcagct gtggctgcgc atagcagaca tacaacggac ggtgggccca gacccaggct 2880

2821 gtcaagtcga caccgacgcg tatcgtctgt atgttgcctg ccacccgggt ctgggtccga 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gtgtagaccc agcccccccg ccccgcagtg cctaggtcac ccactaacgc cccaggcctg 2940

2881 cacatctggg tcgggggggc ggggcgtcac ggatccagtg ggtgattgcg gggtccggac 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gtcttggctg ggcgtgactg ttaccctcaa aagcaggcag ctccagggta aaaggtgccc 3000

2941 cagaaccgac ccgcactgac aatgggagtt ttcgtccgtc gaggtcccat tttccacggg 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tgccctgtag agcccacctt ccttcccagg gctgcggctg ggtaggtttg tagccttcat 3060

3001 acgggacatc tcgggtggaa ggaagggtcc cgacgccgac ccatccaaac atcggaagta 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cacgggccac ctccagccac tggaccgctg gcccctgccc tgtcctgggg agtgtggtcc 3120

3061 gtgcccggtg gaggtcggtg acctggcgac cggggacggg acaggacccc tcacaccagg 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tgcgacttct aagtggccgc aagccacctg actcccccaa caccacactc tacctctcaa 3180

3121 acgctgaaga ttcaccggcg ttcggtggac tgagggggtt gtggtgtgag atggagagtt 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... NheI(3219) .......... ..........

3181 gcccaggtct ctccctagtg acccacccag cacatttagc tagctgagcc ccacagccag 3240

3181 cgggtccaga gagggatcac tgggtgggtc gtgtaaatcg atcgactcgg ggtgtcggtc 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 aggtcctcag gccctgcttt cagggcagtt gctctgaagt cggcaagggg gagtgactgc 3300

3241 tccaggagtc cgggacgaaa gtcccgtcaa cgagacttca gccgttcccc ctcactgacg 3300

.......... .......... .......... .......... .......... ..........

.......... .......... SacI(3327) .......... .......... ..........

3301 ctggccactc catgccctcc aagagctcct tctgcaggag cgtacagaac ccagggccct 3360

3301 gaccggtgag gtacgggagg ttctcgagga agacgtcctc gcatgtcttg ggtcccggga 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ggcacccgtg cagaccctgg cccaccccac ctgggcgctc agtgcccaag agatgtccac 3420

3361 ccgtgggcac gtctgggacc gggtggggtg gacccgcgag tcacgggttc tctacaggtg 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 acctaggatg tcccgcggtg ggtggggggc ccgagagacg ggcaggccgg gggcaggcct 3480

3421 tggatcctac agggcgccac ccaccccccg ggctctctgc ccgtccggcc cccgtccgga 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ggccatgcgg ggccgaaccg ggcactgccc agcgtggggc gcgggggcca cggcgcgcgc 3540

3481 ccggtacgcc ccggcttggc ccgtgacggg tcgcaccccg cgcccccggt gccgcgcgcg 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ccccagcccc cgggcccagc accccaaggc ggccaacgcc aaaactctcc ctcctcctct 3600

3541 ggggtcgggg gcccgggtcg tggggttccg ccggttgcgg ttttgagagg gaggaggaga 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tcctcaatct cgctctcgct cttttttttt ttcgcaaaag gaggggagag ggggtaaaaa 3660

3601 aggagttaga gcgagagcga gaaaaaaaaa aagcgttttc ctcccctctc ccccattttt 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 aatgctgcac tgtgcggcga agccggtgag tgagcggcgc ggggccaatc agcgtgcgcc 3720

3661 ttacgacgtg acacgccgct tcggccactc actcgccgcg ccccggttag tcgcacgcgg 3720

.......... .......... .......... .......... .......... ..........

.......... .......... XhoI(3744) .......... .......... ..........

3721 gttccgaaag ttgcctttta tggctcgagc ggccgcggcg gcgccctata aaacccagcg 3780

3721 caaggctttc aacggaaaat accgagctcg ccggcgccgc cgcgggatat tttgggtcgc 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gcgcgacgcg ccaccaccgc cgagaccgcg tccgccccgc gagcacagag cctcgccttt 3840

3781 cgcgctgcgc ggtggtggcg gctctggcgc aggcggggcg ctcgtgtctc ggagcggaaa 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gccgatccgc cgcccgtcca cacccgccgc caggtaagcc cggccagccg accggggcat 3900

3841 cggctaggcg gcgggcaggt gtgggcggcg gtccattcgg gccggtcggc tggccccgta 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gcggccgcgg ccccttcgcc cgtgcagagc cgccgtctgg gccgcagcgg ggggcgcatg 3960

3901 cgccggcgcc ggggaagcgg gcacgtctcg gcggcagacc cggcgtcgcc ccccgcgtac 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gggggggaac cggaccgccg tggggggcgc gggagaagcc cctgggcctc cggagatggg 4020

3961 cccccccttg gcctggcggc accccccgcg ccctcttcgg ggacccggag gcctctaccc 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ggacacccca cgccagttcg gaggcgcgag gccgcgctcg ggaggcgcgc tccgggggtg 4080

4021 cctgtggggt gcggtcaagc ctccgcgctc cggcgcgagc cctccgcgcg aggcccccac 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ccgctctcgg ggcgggggca accggcgggg tctttgtctg agccgggctc ttgccaatgg 4140

4081 ggcgagagcc ccgcccccgt tggccgcccc agaaacagac tcggcccgag aacggttacc 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ggatcgcagg gtgggcgcgg cgaagccccc gccaggcccg gtgggggctg gggcgccatt 4200

4141 cctagcgtcc cacccgcgcc gcttcggggg cggtccgggc cacccccgac cccgcggtaa 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gcgcgtgcgc gctggtcctt tgggcgctaa ctgcgtgcgc gctgggaatt ggcgctaatt 4260

4201 cgcgcacgcg cgaccaggaa acccgcgatt gacgcacgcg cgacccttaa ccgcgattaa 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gcgcgtgcgc gctgggactc aaggcgctaa atgcgcgtgc gttctggggc ccggggtgcc 4320

4261 cgcgcacgcg cgaccctgag ttccgcgatt tacgcgcacg caagaccccg ggccccacgg 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gcggcctggg ctggggcgaa ggcgggctcg gccggaaggg gtggggtcgc cgcggctccc 4380

4321 cgccggaccc gaccccgctt ccgcccgagc cggccttccc caccccagcg gcgccgaggg 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gggcgcttgc gcgcacttcc tgcccgagcc gctggccgcc cgagggtgtg gccgctgcgt 4440

4381 cccgcgaacg cgcgtgaagg acgggctcgg cgaccggcgg gctcccacac cggcgacgca 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gcgcgcgcgc cgacccggcg ctgtttgaac cgggcggagg cggggctggc gcccggttgg 4500

4441 cgcgcgcgcg gctgggccgc gacaaacttg gcccgcctcc gccccgaccg cgggccaacc 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gagggggttg gggcctggct tcctgccgcg cgccgcgggg acgcctccga ccagtgtttg 4560

4501 ctcccccaac cccggaccga aggacggcgc gcggcgcccc tgcggaggct ggtcacaaac 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ccttttatgg taataacgcg gccggcccgg cttcctttgt ccccaatctg ggcgcgcgcc 4620

4561 ggaaaatacc attattgcgc cggccgggcc gaaggaaaca ggggttagac ccgcgcgcgg 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ggcgccccct ggcggcctaa ggactcggcg cgccggaagt ggccagggcg ggggcgacct 4680

4621 ccgcggggga ccgccggatt cctgagccgc gcggccttca ccggtcccgc ccccgctgga 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 cggctcacag cgcgcccggc tattctcgca gctcacatcg aaggtaaacg aattccccca 4740

4681 gccgagtgtc gcgcgggccg ataagagcgt cgagtgtagc ttccatttgc ttaagggggt 4740

.......... .......... .......... .......... .......... ..........

NcoI(4741) .......... .......... .......... .......... ..........

4741 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg 4800

4741 ggtaccactc gttcccgctc ctcgacaagt ggccccacca cgggtaggac cagctcgacc 4800

.......... .......... ....... EGFP Orf frame 3 ..... EGFP_N_primer

.......... .......... .......... .......... .......... ..........

4801 acggcgacgt aaacggccac aagttcagcg tgtctggcga gggcgagggc gatgccacct 4860

4801 tgccgctgca tttgccggtg ttcaagtcgc acagaccgct cccgctcccg ctacggtgga 4860

EGFP_N_primer ... EGFP Orf frame 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca 4920

4861 tgccgttcga ctgggacttc aagtagacgt ggtggccgtt cgacgggcac gggaccgggt 4920

.......... ....... EGFP Orf frame 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga 4980

4921 gggagcactg gtgggactgg atgccgcacg tcacgaagtc ggcgatgggg ctggtgtact 4980

.......... ....... EGFP Orf frame 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct 5040

4981 tcgtcgtgct gaagaagttc aggcggtacg ggcttccgat gcaggtcctc gcgtggtaga 5040

.......... ....... EGFP Orf frame 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc 5100

5041 agaagttcct gctgccgttg atgttctggg cgcggctcca cttcaagctc ccgctgtggg 5100

.......... ....... EGFP Orf frame 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc 5160

5101 accacttggc gtagctcgac ttcccgtagc tgaagttcct cctgccgttg taggaccccg 5160

.......... ....... EGFP Orf frame 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga 5220

5161 tgttcgacct catgttgatg ttgtcggtgt tgcagatata gtaccggctg ttcgtcttct 5220

.......... ....... EGFP Orf frame 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 acggcatcaa ggcgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg 5280

5221 tgccgtagtt ccgcttgaag ttctaggcgg tgttgtagct cctgccgtcg cacgtcgagc 5280

.......... ....... EGFP Orf frame 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc 5340

5281 ggctggtgat ggtcgtcttg tgggggtagc cgctgccggg gcacgacgac gggctgttgg 5340

.......... ....... EGFP Orf frame 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg 5400

5341 tgatggactc gtgggtcagg cgggactcgt ttctggggtt gctcttcgcg ctagtgtacc 5400

.......... ....... EGFP Orf frame 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt 5460

5401 aggacgacct caagcactgg cggcggccct agtgagagcc gtacctgctc gacatgttca 5460

EGFP_C_primer ... EGFP Orf frame 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 aatgaattaa ttaagaatta tcaccgcttc tattcagcca gtaaggcctg tctttaatgg 5520

5461 ttacttaatt aattcttaat agtggcgaag ataagtcggt cattccggac agaaattacc 5520

.......... .......... .......... .......... .......... ..........

.......... XbaI(5540) .......... .......... .......... ..........

5521 cctccggcat gagacacttt ctagaattcg gtaccccccc ccccaacgtt actggccgaa 5580

5521 ggaggccgta ctctgtgaaa gatcttaagc catggggggg ggggttgcaa tgaccggctt 5580

.......... .......... .......... .......... .......... IRES

.......... .......... .......... .......... .......... ..........

5581 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt 5640

5581 cggcgaacct tattccggcc acacgcaaac agatatacaa taaaaggtgg tataacggca 5640

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg 5700

5641 gaaaaccgtt acactcccgg gcctttggac cgggacagaa gaactgctcg taaggatccc 5700

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc 5760

5701 cagaaagggg agagcggttt ccttacgttc cagacaactt acagcacttc cttcgtcaag 5760

.......... .......... IRES .......... .......... ..........

HindIII(5767) ...... .......... .......... .......... ..........

5761 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc 5820

5761 gagaccttcg aagaacttct gtttgttgca gacatcgctg ggaaacgtcc gtcgccttgg 5820

.......... .......... IRES .......... .......... ..........

.......... .......... .......... PmlI(5859) .......... ..........

5821 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa 5880

5821 ggggtggacc gctgtccacg gagacgccgg ttttcggtgc acatattcta tgtggacgtt 5880

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc 5940

5881 tccgccgtgt tggggtcacg gtgcaacact caacctatca acacctttct cagtttaccg 5940

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg 6000

5941 agaggagttc gcataagttg ttccccgact tcctacgggt cttccatggg gtaacatacc 6000

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac 6060

6001 ctagactaga ccccggagcc acgtgtacga aatgtacaca aatcagctcc aatttttttg 6060

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 gtctaggccc cccgaaccac ggggacgtgg tttcctaatt tttagcaggc tgatgggtgt 6120

6061 cagatccggg gggcttggtg cccctgcacc aaaggattaa aaatcgtccg actacccaca 6120

.......... IRES .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 gtaatgatat ctctctgtgg ttaattacca gtcatgccat acgcttttta atgtttatta 6180

6121 cattactata gagagacacc aattaatggt cagtacggta tgcgaaaaat tacaaataat 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 gctatgtgtg aactgcttgt taaagacttt tgcccattgt tggcaaaagg ttgtctcttc 6240

6181 cgatacacac ttgacgaaca atttctgaaa acgggtaaca accgttttcc aacagagaag 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 gtttatttgt tggaattttt tgtttgtttg tttgttttta aatacaggtg ggggtctcag 6300

6241 caaataaaca accttaaaaa acaaacaaac aaacaaaaat ttatgtccac ccccagagtc 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 tatgttaccc aggctggtct caaacttctg ggctcaagtg atcctcctgc ctctgccttc 6360

6301 atacaatggg tccgaccaga gtttgaagac ccgagttcac taggaggacg gagacggaag 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 tcaaagaact gggattacag gcatgagcca ttatgcccag cctattggta gaagttcttt 6420

6361 agtttcttga ccctaatgtc cgtactcggt aatacgggtc ggataaccat cttcaagaaa 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 atatgttttg aatatttatc gcacatttag gcatccagtt ttgtgaaagc cctcagtttt 6480

6421 tatacaaaac ttataaatag cgtgtaaatc cgtaggtcaa aacactttcg ggagtcaaaa 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 ggatcacttt tatattagtt ttttttttag atcagtttgt agaagttctt tatatatttt 6540

6481 cctagtgaaa atataatcaa aaaaaaaatc tagtcaaaca tcttcaagaa atatataaaa 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 tgatatgact gctttatcag acccatgtac agcaaatatt ttctcccact ctttggtttg 6600

6541 actatactga cgaaatagtc tgggtacatg tcgtttataa aagagggtga gaaaccaaac 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 cctttccact ctttcggtga tgtcttttgg tgaacagaag tttttaattt tactacagtc 6660

6601 ggaaaggtga gaaagccact acagaaaacc acttgtcttc aaaaattaaa atgatgtcag 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 ccatgtatga attgttttaa ttatgattac tgcctttcaa aaacatttaa gaaagtttta 6720

6661 ggtacatact taacaaaatt aatactaatg acggaaagtt tttgtaaatt ctttcaaaat 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 cctactcaaa gtttatgaaa atattcttct cctatgctct cttctaaaat atgtattgct 6780

6721 ggatgagttt caaatacttt tataagaaga ggatacgaga gaagatttta tacataacga 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 ttacctttcc aattttaagg ataatacaac tgaaactaac tttttttaaa agtaactttt 6840

6781 aatggaaagg ttaaaattcc tattatgttg actttgattg aaaaaaattt tcattgaaaa 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 aggctgggcg tggtggctca cgcctgtaat cccagtactt caggaggccg acattgggga 6900

6841 tccgacccgc accaccgagt gcggacatta gggtcatgaa gtcctccggc tgtaacccct 6900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 tatcacctga ggtcaggagt tcgagaccag cctggccaac atggtgaaac tccatatcta 6960

6901 atagtggact ccagtcctca agctctggtc ggaccggttg taccactttg aggtatagat 6960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 ctaaaaacaa aacttagcca ggtgtggtgg cagacacctg taatcccagc tactcgggag 7020

6961 gatttttgtt ttgaatcggt ccacaccacc gtctgtggac attagggtcg atgagccctc 7020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 gctgaggcag gagaatcact tgaacccagg aggcggaggt tgcagtgagc cgagattatg 7080

7021 cgactccgtc ctcttagtga acttgggtcc tccgcctcca acgtcactcg gctctaatac 7080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 ccattgcact ccagcctggg tgacagagca agactccatc tcaaaaagaa ataaataaat 7140

7081 ggtaacgtga ggtcggaccc actgtctcgt tctgaggtag agtttttctt tatttattta 7140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 aaaaaataaa agtaactttt aaatgattgt gaggtaggga tcaagtttaa tgtttttcca 7200

7141 ttttttattt tcattgaaaa tttactaaca ctccatccct agttcaaatt acaaaaaggt 7200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 tagggatatt caattggccc atcccaaaaa gaccggcctt tctccactac acagcagtgt 7260

7201 atccctataa gttaaccggg tagggttttt ctggccggaa agaggtgatg tgtcgtcaca 7260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 tgactttgtc atctatcagg tgacccttca gcctgtctgt ggactctttg ttgtgttcca 7320

7261 actgaaacag tagatagtcc actgggaagt cggacagaca cctgagaaac aacacaaggt 7320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 ttggcctgtt tgtctattgt tggaacaaaa ccacactgtc tgaattgcca cagctttata 7380

7321 aaccggacaa acagataaca accttgtttt ggtgtgacag acttaacggt gtcgaaatat 7380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 atagggcatg ctatcaagca gtcagtgtca ttttacaggt ttgttcttct ttaggtttgc 7440

7381 tatcccgtac gatagttcgt cagtcacagt aaaatgtcca aacaagaaga aatccaaacg 7440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 cttagctatt ctttggtctt tgcattgcca tctaaatttt agaatcaact aagtcaaaat 7500

7441 gaatcgataa gaaaccagaa acgtaacggt agatttaaaa tcttagttga ttcagtttta 7500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 gttgtatcac tttccacacc aaaaatctgc tggaattttg attgggatta tattaaattt 7560

7501 caacatagtg aaaggtgtgg tttttagacg accttaaaac taaccctaat ataatttaaa 7560

.......... .......... .......... .......... .......... ..........

.......... ......... BglII(7584) ......... .......... ..........

7561 gtagatcaat ttgggaagaa ataagatctt tacaacatta actcttccaa tctataaatg 7620

7561 catctagtta aacccttctt tattctagaa atgttgtaat tgagaaggtt agatatttac 7620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 tggtatttat tttttcttta tttttttctc agtggtttgt agtttctagt gcaaaggtct 7680

7621 accataaata aaaaagaaat aaaaaaagag tcaccaaaca tcaaagatca cgtttccaga 7680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 ttgacatcgt tagatttaat gttttttgat atattttggt gttataagtg atacttttca 7740

7681 aactgtagca atctaaatta caaaaaacta tataaaacca caatattcac tatgaaaagt 7740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 aaatttcatt ttctatttgt ttcttgttga cacagcaaca caactggttt tagtgtaata 7800

7741 tttaaagtaa aagataaaca aagaacaact gtgtcgttgt gttgaccaaa atcacattat 7800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 atcttatatt tagagccctt gctaattaaa cctattaatt ataatagttc ttctatagat 7860

7801 tagaatataa atctcgggaa cgattaattt ggataattaa tattatcaag aagatatcta 7860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7861 tctttcagct tttttgcata tacgatcatg tcatctgtga gtaatgacat gaacccagga 7920

7861 agaaagtcga aaaaacgtat atgctagtac agtagacact cattactgta cttgggtcct 7920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7921 ggcgttcttt ccaggctttc tttttatagt catgtactgc ttaacgacgt ttttgtcgat 7980

7921 ccgcaagaaa ggtccgaaag aaaaatatca gtacatgacg aattgctgca aaaacagcta 7980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 gatgaactgc atatacaaca gtggtcccat aagattataa agaggctgaa aaattcttac 8040

7981 ctacttgacg tatatgttgt caccagggta ttctaatatt tctccgactt tttaagaatg 8040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8041 tgcctagtga cactgtagcc atcataatgt agcacaattc agtac..... .......... 8085

8041 acggatcact gtgacatcgg tagtattaca tcgtgttaag tcatg..... .......... 8085

.......... .......... .......... .......... .......... ..........