8"C- Bluescribe "Times New RomanC-C 3 pGEX_3_primer"Times New RomanC-C lacZ_a"Times New Roman<C-CɆ lM13_pUC_fwd_primer"Times New RomanxC- {M13_forward20_primer"Times New RomanxC-C  T7_promoter"Times New RomanC-C  T3_promoter"Times New RomanxC-CɆ M13_reverse_primer"Times New Roman<C- 0M13_pUC_rev_primer"Times New RomanC-C >[ lac_promoter"Times New Roman<C-C  pBR322_origin"Times New RomanC-CɆ   Orf frame 3"Times New Roman<C-   Ampicillin"Times New RomanC-C  8  AmpR_promoter"Times New Roman C-NdeI"Times New Roman XB/ / C-NarI"Times New Roman & cR!@!1X@ C-eEcorI(423), SacI(433), KpnI(439),SmaI(441), BamHI(444), Xbal(450), SalI(456), PstI(466), HindIII(474)"Times New Roman, `  C-AatII"Times New Romany  lF]:K. K.