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The Relation of Intracellular F-actin Content and Cellular Morphology of SHEE Cell
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CAI Wei-jia1, CHEN Yao-wen2£¬XU Li-yan 1, LIN Jue-long2, LI En-min 3*
(1.Department of Tumor Pathology, Medical College, Shantou University£¬ Shantou 515031, Guangdong, China£»2. Center Laboratory, Medical College, Shantou University£¬Shantou 515031, Guangdong, China£»3. Department of Biochemistry and Molecular Biology,Medical College,Shantou University£¬Shantou 515031, Guangdong, China)
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Abstract: Study to change of SHEE cellular morphology passing by different culture medium. Using the technologies of microscopy and phalloidin-FITC labeling and flow cytometry, SHEE cell was tested passing by accordant and alterative culture medium. The result show that cell membrane display abnormity and cellular connecting structure loose and intracellular F-actin content reduce passing by alterative culture medium. This account for cytoskeleton and cellular morphology and adhering capacity could be affected by alterative culture medium in the process of the culture.
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