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Effects of Free Calcium in H292 Cells through Protease-activated Receptor-2
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WANG Hai-yan, HE Shao-heng, LIN Jue-long
(Allergy and Inflammation Research Institute, Shantou University Medical College, Shantou 515041, China)
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Abstract£ºObjective: To study the effects of PAR-2 agonists SLIGKV and tc-LIGRLO, trypsin and its inhibitors on intracellular calcium concentration [Ca2+]i in the H292 lung epithelial cell. Methods: Fluo-3/AM fluorescence technique and laser scanning confocal microscope (LSCM) were used to measure the intracellular calcium level after different reagents were added in Hanks of H292 cells. Results: Trypsin£¬SLIGKV, tc-LIGRLO could increase the intracellular calcium concentration [Ca2+]i in H292 cells. Compared with control, the average fluorescent intensities were increased 267£¥,60£¥,37£¥, respectively.Trypsin inhibitors soybean trypsin inhibitor(SBTI) and ¦Á1-antitrypsin(¦Á1-AT) could inhibit trypsin induced the increase of [Ca2+]i in H292 cells. Conclusion: Activation of protease-activated receptor-2 induce the increase of [Ca2+]i in H292 cells. Trypsin inhibitors could inhibit trypsin induced the increase of [Ca2+]i in H292 cells.
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