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The Observation of the Influence of Ionizing Radiation on the Image of F-actin in Vascular Endothelial Cells with Confocal Laser Scanning Microscope
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CHEN Shi-sheng1, YAO Xiao-wu1, YANG Li-he1, LIN Jue-Long2, YANG Hai-wei2
(1.The Second Affiliated Hospital of Shantou University, Shantou 515041, Guangdong, China; 2.Shantou University Medical college Central Laboratory,Shantou 515041, Guangdong, China)
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Abstract:Objective:To observe the influence of ionizibg radiation on the F-actin in vascular endothelial cells and investigate mechanism of ionizing radiation injury on vascular endothelial cells. Method: HUVE-12 cells was cultured in vitro and exposed to Co60 γ ray with 0、2、4、6、8、10、12Gy. The image of F-actin in HUVE-12 cells was observed with Confocal Laser Scanning Microscope at 6 hours after radiation. Results: radiation-induced depolymerization of F-actin microfilament stress fibres was dependent on radiation dose. Conclusion: radiation-induced depolymerization of F-actin microfilament stress fibres may be one of the mechanism of the radiation injury on vascular endothelial cells.
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