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Subcellular Location of GAS41 by its Expression and Preparation of Antiserum
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DING Xiao-feng, HU Xiang, ZHANG Jian*
（College of Life Science, Hunan Normal University， Changsha 410081, Hunan, China）
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Abstract:GAS41 is a recently identified gene slightly related to glioma. In order to study the expression, subcellular distribution and the function of GAS41 protein，the coding region of human GAS41 gene was successfully subcloned into the prokaryotic expression vector pQE-N3，The vector was then introduced into E. coli BL21（DE3）cells for highly efficient expression. His-fusion protein of 30 kD was detected in the inclusion body. After refolded and purified，this protein was used to immunize new Zealand white rabbit to produce polyclonal antibodies. The specificity of the anti-GAS41 antibody was successfully proved by Western blotting and analysis on both of the prokaryotic and eukaryotic expressed products of GAS41 protein.Moreover, the GAS41 immunoreactive cells were found to be uniformly distributed in the nucleolus,this suggests GAS41 may be an important transcription factor.
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