Effects of Fructo-oligosaccharides and Mannan Oligosaccharides on Immune Response in Weaned Pigs

Abstract: The effects of Fructo-oligosaccharides(FOS) and Mannan-oligosaccharides (MOS) on immune response was studied in weaned pigs. Fourty-eight 35-d-old weaned Duroc×Landrace-Yorkshire pigs were divided into four groups with three replicates, each of which including 4 piglets, in a randomized block design. The four groups were fed with basic diet containing 0.5%FOS(group FOS), containing 0.3%MOS(group MOS), containing 0.45%FOS and 0.25MOS(group F+M), containing 75mg/kg Chlorcycline and 40mg/kg Roxarsone(group ABT), respectively. The results showed that: (1)The index of thymus, spleen, lymph node in group FOS, MOS, F+M were higher than that in group ABT(P﹥0.05). (2)In 7th day after weaned, the concentrations of immunoglobulin(IgA) in group FOS(0.32±0.08), MOS(0.32±0.04), F+M(0.32±0.07) significantly higher than in group ABT (0.20±0.01) (p﹤0.05), at the same time, the concentrations of IgG in group FOS(3.50±0.49) were higher than that in group ABT(2.68±0.47) (p﹤0.05); Compared with antibiotics, F+M increased the concentration of IgG markedly(p﹤0.05) in 21th day after weaned; MOS increased the concentration of IgM profoundly(p﹤0.05) in 21th day after weaned. (3)In 7th day after weaned, the percentages of CD4+ of thymus, spleen, lymph node in group FOS(37.33±4.04, 30.33±8.73, 37.67±2.52) were higher than in group ABT(21.33±6.03, 19.67±2.08, 24.67±4.16 accordingly) (p﹤0.05), the percentages of CD8+ of spleen in group F+M(22.66±3.05) were higher than in group ABT(17.00±1.00) (p﹤0.05), the percentages of CD8+ of thymus in group MOS(29.67±5.51)were higher than that in group ABT(20.67±3.51), the percentages of CD8+ of lymph node in group F+M(20.66±6.42) were lower than that in group ABT(34.33±5.03) (p﹤0.05); In 28th day after weaned, the percentages of CD4+ of thymus in group FOS(32.00±5.29), MOS(37.33±3.21), F+M(32.00±3.60) were higher than in group ABT(21.33±4.04) (p﹤0.05), there were no significant differences among group FOS, MOS, F+M and antibiotics(P﹥0.05) in CD8+ of thymus, spleen and lymph node(P﹥0.05); Though there were no significant difference among group FOS, MOS, F+M and ABT, CD4+/CD8+ ratio in group FOS, MOS,F+M was larger than that in group ABT, except that lympho node CD4+/CD8+ ratio in group FOS(1.42±0.32), MOS(1.52±0.46), F+M(1.51±0.30) was larger (p﹤0.05)than that in group ABT(0.71±0.03) in 7th day after weaned and spleen CD4+/CD8+ ratio in group FOS(1.36±0.21), MOS(1.34±0.16) was larger(p=0.01) than in group ABT(0.94±0.29).