Abstract: SPATA17 gene, homologue to mouse MSRG-11 gene, was cloned by informatics analysis. The cDNA mapped to chromosome 1q41 in the human genome is made up of 11 exons and the exon-intron boundaries obey to the AG/GT rule. The gene contains a 1083 bp open reading frame encoding 361 amino acid sequences with theoretical molecular weight of 47.49 KD and isoelectric point of 9.75. The protein encoded by this gene contains three highly conserved IQ motifs and is a new member of IQ protein family. One CpG island is located in the gene from site -176 to -659. A typical promoter is also predicted from site -828 to -928. According to the computer-aided analysis of the putative protein encoded by the SPATA17, no transmembrane region and no signal peptides are found in the protein. Multi-tissue RT-PCR and northern results show that the SPATA17 transcript of 2.0 kb was detected strongly in adult human testis only. In conclusion, this study predicts its important role in spermatogenesis cell apoptosis