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Construction of DNA Vaccine for Swine Hepatitis E Virusand Immunology Effect on Mice
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ZHAO Yu-jun1£¬ZHU Yuan-mao2£¬XIANG Wen-hua2
(1£®College of Animal Science of Shanxi Agriculture University , Taigu 030801, Shanxi, China; 2.Haerbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Haerbin 050001, Heilongjiang, China)
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Abstract: Using swine HEV DQ1strain ORF2 as template, and a pair of primer with kozak sequence , by PCR we got whole ORF2 of swine HEV DQ1. By Inserting the amplified part into vaccine vector pcDNA3.1, we constructed the DNA vaccine for swine HEV DQ1. After translated DNA vaccine into vero cell and immunized BAL B/C mice ,we found that the vaccine could produce protein detected by immunofluorscence assay in vero cells and induce immunology reaction in mice.
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