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Construction and Transformation of Maize Starch Branching Enzyme SBE¢òb Gene RNA Interference Expression Vector
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XIANG Yan,£¬ ZHANG Yong-Feng,£¬ JIANG Hai-Yang,£¬
HAN Guo-Min £¬ZHU Su-wen£¬ CHENG Bei-jiu*
(Anhui Agricultural University , Hefei 230036£¬Anhui, China)
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Abstract: Twentieth extron (155 bp) from genome of sbe¢òb gene have been amplified. The positive oriention and negative oriention of the fragment is ligated into pUCRNAi. Vector p1300+HMW+2F have been constructed by utilizing pHMW¡¤GUS and pCAMBIA1300 vector which respectively contain hygromycin selecting gene. Through the tri-parental mating experiment ,the RNAi expression vector pCAMBIA1300+HMW+2F has been transformed into A.tumefaciens LBA4404. Genetic transformation into maize inbred lines 178 ,four transgenic maize are obtained by hygromycin selecting and PCR analysis.
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