Abstract: The purpose of this study was analysis and tracking the heredity stability of Alpaca through amplifying special fragment from mitochondrion genome. A pair of primer was designed according to those sequences of Camelidae animal submitted in GaneBank and a fragment of 1014 bp was cloned. Analyzing by blastn program of NCBI, the fragment includes: 30bp 12srRNA partial sequence, 71bp tRNA-Val complete sequence and 913bp 16srRNA partial sequence, and the homolog is more than 95 % compared with other Camelidae animals. The secondary structure of tRNA-Val was mapped using RNAstructure 4.2. The results showed that the amino acid arm and anticodon loop of tRNA-Val is conserved among genus.