Abstract: Exogenous hormones in medium for propogation of Pyrus.spp plantlet caused endogenous hormones changed and led to subculture plantlet proliferating and growing. Content of ZR increased and there was a significant negative correlation between the rate of IAA/ZR and effective proliferation shoots in BA treated. A significant increase in IAA level when NAA treated, which brought a greater shoot growth. Content of GA1＋3 was found in initial subculture plantlet, which increased in suitable GA3 treated, meanwhile the content of ABA dropped significantly and thus promoting the shoot growth and area of leaves.