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Breeding and Characterization of Phosphorus-accumulating Strains by Ions Implantation
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TANGGui-lan1, HUA Ri-mao1, Sun Zhen-jun2 , WANG Jian1
(1.AnHui Agriculture University,HeFei 230036, China 2. College of Resources and Environmental Sciences,China Agriculture University.BeiJing 100094,china)
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Abstract£ºAfter irradiation of N+ ion beam at 15KeV on the G strain,the two mutant strain K1¡¢K2 were obtained. Their accumulate ratio of phosphorus was increased by 1.43~3.89 times than that of original.Through morphological and biochemical method,the two strains were identified as Pseudomonas spp.The tests of culture conditions showed that the optimal temperature to two strains was 30¡æ.The optimal pH to K1 was 6, it was 8 to K2.The optimal time to K1was 3d and it was 4d to K2. The optimal OD to K1was 0.6 and it was 0.4 to K2¡£
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