Abstract: The experiment was designed to study the mutation regulation on secondary branch fruit quality of appearance and flavor after 60Co γ ray irradiated on the loquat Jiefanghong branch. The result showed that the γ ray from 14.4Gy to 28.8Gy dose had great mutagenic effect on fruit characters, such as flavor, fresh color, pericarp color, rusty spot, fleshy, juice, et al. The frequency was from 0.48 percent to 44.06 percent. The effect was flavor, flesh color, pericarp color, fleshy, rusty, juice in turns. And the beneficial was flavor, pericarp color, juice, fleshy in turns. Compared synthetically, it was suitable to select the irradiated dose of 24.0Gy, to improve the loquat Jiefangzhong fruit quality of appearance and flavor.