Abstract：According to the sequence of the published delta-12 desaturase genes of maize（GeneBank ID：AB257309）, a specific cDNA fragment was amplified by RT-PCR from the total RNA of maize immature embryos. The intron-spliced hairpin RNA plant expression vector pCAMBIA1300＋UFIFN was constructed succesessfully, incorporating Ubiquitin promoter and the 120 bp specific fragment. The embryogenic calli were initiated from hybrid lines of high oil maize breeded by ourselves, and transformed by Agrobacterium tumefaciens strain LBA4404 carrying pCAMBIA1300＋UFIFN vector. Six positive transgenic plants were obtained by the PCR analysis using hpt-specific primers.