Abstract：14 cry1Ac genes from GenBank were aligned and the consensus regions in the upstream of promoter and in the downstream of terminator were found. Based on the consensus sequences, a pair of primers was designed and a 4.2 kb element was amplified that includes the dual overlapping promoter and the whole termination-associated sequence from Bacillus thuringiensis strain 4.0718, and the amplified 4.2 kb element was confirmed to contain the cry1Ac gene by cryI sub-genetype PCR-RFLP cry gene typing system. The 4.2 kb element was cloned into Bt-E.coli shuttle vector pHT304 and the cry1Ac gene was also expressed in E.coli DH5α and acrystalliferous mutant XZM-101. Meanwhile, rhombic crystal was observed from recombinant strain BXZM34 by atomic force microscope.