Abstract: The Dmrt genes constitute a new gene family related to sex-determination. They encoded transcription factors characterized by a conserved zinc finger-like DNA-binding motif, the DM domain. In this paper, degenerate PCR was used to amplify the conserved DM domains of Dmrt genes in Eremias breuchleyi. Then , seven different DM sequences were identified and named as EbDmrt1a, EbDmrt1b, EbDmrt1c, EbDmrt1d, EbDmrt3, EbDmrt4 and EbDmrt5 according to its name Eremias breuchleyi. The results indicated that Dmrt genes were highly conservative in phylogeny and have several members.