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Cloning of a Rice Endosperm-specific Promoter Gt1 and Its Functional Verification in Maize
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QIAO Guang-ming1,LI Zhong-cheng1©~,CHEN Zheng-hua2,
WANG Lan-lan2, CHEN Ling-na2, ZHANG Ming-hao2
(1. College of Life Science And Engineering, Southwest University of Science and Technology, Sichuan Mianyang 621010£»2.Postdoctoral Scientific Research Station of Gansu Yasheng Industrial (Group) Co.Ltd, Beijing Branch, Beijing 100101)
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Abstract Regard genome DNA of rice as the template , have cloned the upper sequence of the gluten genetic Gt1 of rice by PCR method , and construct it on the plant expression carriers with green fluorogenic albumen, shell with laster microbeam method receptor to transform maize organize, prove, should start function of son through the method that express instantly£ºIt expresses strongly in endosperm, while very weakly other£»It has proved that has the peculiar characteristic that expresses of endosperm in different species too; It offers the theoretical foundation for the use of this endosperm.-specific promoter.
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