Abstract: The complete sequences of mitochondrial ND1 genes were determined for L. kuhlii and L. fragilis, and they are 978 (codes 325 amino acids) and 958 (codes 319 amino acids) nucleotides long, respectively. Two sequences were deposited in GenBank (accession No. AY899241 and AY899242 ). The authors aligned the ND1 gene sequences of them and L. fujianensis (the published sequence in GenBank, AY974191) using Clustal X software program. By comparing three sequences of Limnonectes, the divergences and base substitutions among these sequences were analyzed with the MEGA3.1 software. The alignment of the ND1 gene contained 978 nucleotide positions with gaps, including 664 constant sites and 294 variable sites. The ND1 gene sequence of L. kuhlii showed minor differences (92.62﹪ similarity at the amino acid level and 78.77﹪ similarity at the nucleotides level) to the homologous sequence of L. fujianensis mt DNA. To further confirm the Phylogenetic relationships among 3 Chinese species of Limnonectes, another 2 mt ND1 genes of frogs (F. limnocharis and R. nigromaculata) were retrieved from GenBank, the salamander (Andrias davidianus, AJ492192) was used the outgroup species. DNA molecular phylogenies were derived using MEGA3.1, with neighbor-joining analyses, based on the amino acid sequence and the nucleotides sequences data, respectively. The phylogenetic analyses results show that two trees of NJ both strongly support a monophyletic group of 3 Chinese species of Limnonectes and a sister group relationship for L. kuhlii and L. fujianensis. A close relationship between L. kuhlii and L. fujianensis was confired.