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Isolation, Purification and Structure Characterization of Polysaccharide POP¢óa from Portulace oleracea L.
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ZHU Dan1£¬N IU Guang-cai 1£¬SUN Xi-yun2£¬MENG Xian-jun2
(1.Heilongjiang August First Land Reclamation University, Daqing 163319, Helongjian, China)
(2. Food College of shenyang Agricultural University, shenyang 110161, Helongjiang, China)
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Abstract: POP¢óa, one component of Portulace oleracea L. polysaccharides(POP), was obtained from Portulace oleracea L. by a series of steps of extraction, separation and purification. POP¢óa was homogeneous molecular weight assayed by cellulose acetate film eletrophoresis and sephadex G-200 column chromatography. Monosaccharide composition analysis by TLC and NMR showed that it was composed of arabinose and galacturonic acid. There were characteristic absorption bands of polysaccharide and ¦Â-glycoside linkages as shown by IR spectrum. Meanwhile, it was pectic polysaccharide based on the results of NMR.
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