Abstract：The Dmrt genes constitute a new gene family related to sex-determination like the doublesex gene of Drosophila and the Mab-3 gene of Caenorhabditis elegents. They regulate similar aspects of sexual development. Human doublesex-related gene DMRT1 has been identified, which also contains the conserved DM-related DNA-binding domain and plays an essential role in gonadal differentiation. And the Dmrt genes have been discovered in a wide range of animal species, such as fish, reptiles, birds and mammals. In this paper, degenerate PCR was used to amplify the conserved DM domains of Dmrt genes in Limnonectes Fujianensis. The PCR products were approximately 140bp. After DNA cloning and sequencing analysis, four different DM sequences were identified and named as LfDmrt1a，LfDmrt1b，LfDmrt3 and LfDmrt5, according to its name Limnonectes Fujianensis. The amino acid sequences encoded by Dmrt genes from different species showed a high degree of sequence homology, as revealed in phylogenic tree constructed. The results further indicated that Dmrt genes were highly conservative in phylogeny and the strong evolutionary conservation of this gene family may of importance in developmental process.