Abstract: In order to study the relationship of prolactin receptor gene（PRLR） with reproduction capability of alpacas, and to analyze the homology to other mammals, blood samples from 60 Huacaya alpacas, breed in China Alpacas Base, were taken and their genomic DNAs were extracted by the method of hydroxybenzene / chloroform. The primers were designed to amplify exon8-exon9 of PRLR fragment of alpaca. Sequencing results showed that the fragment covered 622 bp (GenBank accession No. DQ198164), which included partial sequence of exon8 (82 bp), intron8 (472 bp) and partial exon9 (68 bp). And the alignments of the sequence with the corresponding part of other animals (such as cow, sheep, pig, dog, rabbit, rat and so on) was performed, and revealed that the homology of exon8 and exon9 of alpacas PRLR gene was above 92 %. Meanwhile, a single base mutation was detected in the nucleotide of PRLR gene of alpacas, which were G→A at the position of 19 after sense primer, and it lead to amino acid substitution of I→L; and another mutation was at the position of 22 before anti-sense primer, but which located the third base of codon and coding P amino acid. Alpacas are single embryo animals, if the mutation of amino acid is relation to the reproduction capability of alpacas is needed to study further.