Abstract: In order to obtain a large number of wheat haploids to establish the DH (Double Haploid) groups, wheat and maize cross were used to induce wheat haploid embryos and then the haploid plants were produced via young embryo culture. The factors affecting the regeneration of wheat young plants from young embryos，such as different period of peeling embryo，size and development stage of young embryos，the treatment time at 4 ℃ and dark treatment time were studied．The results showed: there was not significant difference on the frequency of young plants production when haploid embryos was peeled in different time from 12 days to 16 days; the frequency of young plants production of the size of 0.5 mm～1.0 mm is much higher than that of the size of 0 mm～0.5 mm and 1.0 mm～1.5 mm; the short time treatment between 1 days～3 days at 4 ℃ would accelerate the regeneration of young embryo, but after 3 days the frequency of callus induction and plants production would decrease; dark treatment of all 24 h for 12 days could effectively improve the frequency of plants production during the process of young embryo culture.