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Construction of Expression Vector with Anti-insect and Antifungal Gene and Study on Transformation Plant by Laser Microbeam
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ZHANG Nan1 , CHEN Zheng-hua2* , LIU Gui-zhen2, CHEN Ling-na1 , QU Yan-ying1
(1. Agronomy College, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China; 2. Postdoctoral Scientific Research Station of Gansu Yasheng Industrial (Group) Co. Ltd, Beijing Branch, Beijing 100101, China)
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Abstract: Antifungal protein gene and Bacillus thuringiesis gene were ligated by the linker of (Gly4Ser)3, which encode a new anti-insect and antifungal protein. The fusion gene (NAMP -Bt) were ligated into prokaryotic expression vector and plant expression vector£®These recombinant plasmids pET-NAMP-Bt and pBI-NAMP-Bt were confirmed by restriction enzyme and PCR analysis and the results showed that these recombinant plasmids were constructed successfully. The fusion gene was induced into tobacco, we had received transgenic tobacco.
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