Abstract: Five male boar goats with 3-4 years old were selected to study the influence of trehalose in Semen Diluents on function and membrane integrity of goat spermatozoa. Goat spermatozoa were diluted(1:4) and frozen in Tris-citric acid-glucose (TCG) diluents with different concentrations of trehalose(0, 6.6, 13.2, 19.8, 26.4, 39.6, 52.9, 66.1, 79.3 mmol/L) respectively. Final concentrations of 18% (v/v) egg yolk and 6% (v/v) glycerol were employed in the diluents. The results showed that, compared with control(0 mmol/L ), sperm motilities of 39.6, 52.9, 66.1, 79.3 mmol/L groups were decreased significantly(P<0.05), abnormal sperm rates of 52.9, 66.1, 79.3 mmol/L groups and swollen sperm rate of 39.6 mmol/L group were increased significantly(P<0.05) after cooling (P<0.05). Compared with control, sperm motilities of 24.6 mmol/L and 39.6mmol/L groups were increased significantly(P<0.05), sperm motility, abnormal sperm rate of 66.1 mmol/L and 79.3 mmol/L groups were decreased and increased significantly(P<0.05) after freezing and thawing respectively. Frozen-thawed sperm capacitation rates that developed from sperm with trehalose were 19.8, 26.4 and 39.6 mmol/L, which were significantly(P<0.05) higher than sperm without trehalose. Frozen-thawed intact acrosomes rate and swollen sperm rate of 39.6 mmol/L trehalose group were significantly(P<0.05) higher than control , but their rates of 66.1 mmol/L, 79.3 mmol/L trehalose groups were decreased significantly(P<0.05) than control proup. Compared with control, frozen-thawed conception rate of 39.6 mmol/L group was increased significantly(P<0.05), but its rate of 66.1 mmol/L and 79.3 mmol/L groups were decreased significantly(P<0.05). The results showed that goat sperm function and membrane integrity were significantly(P<0.05) improved, while adding suitable concentration(26.4 mmol/L or 39.6 mmol/L) trehalose in the TCG diluents with 18% (v/v) egg yolk and 6% (v/v) glycerol.