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Cloning and Sequence Analysis of Serratia marcescens Chitinase chiB Gene
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YE Hui1, CHENG Bei-jiu2*, ZHU Su-wen2, GAN De-fang3
(1. Biotechnology Center of Anhui Agricultural University, Hefei 230036, Anhui, China;
2.School of Life Science, Anhui Agricultural University, Hefei 230036, Anhui, China;
3.School of Horticulture, Anhui Agricultural University, Hefei 230036, Anhui, China;)
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Abstract: The genome DNA was extracted from Serratia marcescens by improved method. A special fragment about 1500bp length was cloned from Serratia marcescens genome DNA by Polymerase Chain Reaction(PCR) amplification. Vector pUC-chiB was constructed through ligating the fragment into the plasmid pUC18 and transformed into E.coli DH5¦Á. Through screening of recombinants and sequence analysis of it, the result showed that the cloned DNA fragment was same to the reported.
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