Abstract: The Sox gene family and Dmrt gene family are act important roles on the development processes of early embryogenesis and sex-determination .The technique of PCR were used in this paper to amplify the Sox gene and Dmrt gene of Rana livida and obtain the fragments about 220 bp and 150 bp. The sequences analysis indicated that the Sox gene and Dmrt gene of Rana livida are no difference between male and female, and have high identities with other animals including human being. The results suggested that the Sox gene and Dmrt gene is high conservative in phylogenesis. The paper supplies molecular data for study the sex-determining mechanism of Rana livida and the evolution of Sox gene and Dmrt gene.