|
|
Cloning of a Sequence Encoding PEP from Brassica napus and Constructing of Gene Antisense Seed-specific Expression Vector and Obtainment of Transgenic Brassica napus Plants
|
|
ZHANG Yan1,LIU Gui-zhen2,CHEN Zheng-hua2,AN Li-zhe1
(1.School of Life Science,Lanzhou University,Gansu,China;2.Postdoctoral Scientific Research Station of the Gansu Yasheng Group,Branch Station of Beijing,Beijing100101,China)
|
|
Abstract: Phosphoenolpyruvate carboxylase (PEP) plays an important role in the control of the ratio of the protein/lipid content in rape (Brassica napus)£®To modify the expression of PEP gene in rape by co-suppression may enhance the oil content of the transgenic plants£®In this research,a PEP gene fragment was amplified from Huayou5(H045) with polymerase chain reaction(PCR) and then cloned into the corresponding sites of the pBI121-B in the antisense orientation£®Antisense PEP gene was introduced into Brassica napus successfully Via laser microbeam puncture,and the transgenic plants had been obtained .
|
|
|
|
|
|
|
|
|
Making Money on the Internet
|
|
|
|
|
Register with us
|
|
BVTech provides high-quality free software.
To keep up with the latest release or help us to improve our products,
please sign our guestbook.
|
|
|
|