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The Study on Oncidium PLBs Propagation in Liquid Culture

CUI Guang-rong, ZHANG Zi-xue, ZHANG Cong-yu, SUN Tao £¨Plant Science Department of Anhui Science and Technology University£¬ Fengyang 233100£¬Anhui£¬China£©


Abstract: The oncidium PLBs(protocorm-like bodies) induced from stem tips were used as explants to cultured in the liquid media which contain hormones, sucrose and coconut palm juice. The effects of different concentrations of hormones , sucrose and coconut palm juice on PLBs propagation were studied in this experiment. Under the same condition, the differences of PLB propagation and shoots regeneration in the liquid culture and solid culture were studied too. The results showed that the concentrations and combinations of hormones have great effect on oncidium PLBs propagation in the liquid culture. The best combination of hormones was 6-BA 0.5 mg/L+Ad 0.05 mg/L. The concentrations of sucrose have also great effect on PLBs propagation. The suitable medium for oncidium PLBs propagation in the liquid culture was 7.5 g/L. The medium added 5ml/l coconut palm juice can not only elevate PLBs propagation coefficient but also improve the quality of oncidium PLBs. The best medium for oncidium PLBs propagation was MS+6-BA 0.5 mg/L+Ad 0.05 mg/L+sucrose 7.5g/L+coconut palm juice 5 mL/L. The PLBs cultured in this medium grew fast, less browning. The PLBs growth curve showed "V" in this liquid medium , while seemed beeline in the solid medium. However, the growth state of shoots regenerated from PLBs cultured in the liquid medium was bad than shoots regenerated from solid medium.

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