Abstract: Objective: observe that if there is difference in micro-emulsion Hycoprellin B (HB) and lipidosome HB in their effectiveness of photodynamic therapy on port wine stains' animal model, and observe the effect and side-effect of photodynamic therapy with these two dosage form. Method: 32 leghorn cock were divided into control group, HMME group, lipidosome 0.5 mg/kg group, lipidosome 1.0 mg/kg group, micro-emulsion 0.5 mg/kg group and micro-emulsion 1.0 mg/kg group. A spot with diameter 1 cm on cock comb was irradiated by 532 nm laser with power density 100 mW/cm2 and energy density 100 J/cm2 after photosensitiser intravenous injection immediately. Visual observation and photos were recorded every day, and pathology was determined 14 days later after photodynamic therapy. Result: 6 cases in HMME group show medium reaction which is decreasing of microvasculature in shallow dermis and light scab. 5 cases in lipidosome-HB 0.5mg/kg group and 6 cases in micro-emulsion 0.5 mg/kg group show severe reaction which is epidermis hyperplasia, decreasing of microvasculature in shallow dermis and medium dark scab. 6 cases in lipidosome-HB 1.0 mg/kg group and 5 cases in micro-emulsion 1.0 mg/kg group show very severe reaction which is necrosis under scar, epidermis hyperplasia, almost no microvasculature in shallow dermis and very thick dark scab. Conclusion: The photodynamic therapy effectiveness with both micro-emulsion HB and lipidosome HB is similar, and has severe damage on microvasculature. But the damage of normal epidermis and dermis is severe too.