Abstract: The biomacromolecule of bovine serum albumin fractionⅤpowder or it's solution was radiated separately by XeCl excimer laser(308nm)in the condition of export out power 25mJ/per pulse or 33.3~34.4 mJ/per pulse, pulse frequency 2 times/s. The radiation samples were set on difference distance:150 mm,250mm,290mm,or 340mm. Far from laser source, and went through different radiation time:15s,30s,45s or 60s. The radiated samples of BSA(Ⅴ) and their control were measured by FT-IR, Vis-UV, and FR, then they were observed and analyzed, and compared with the above two data, The results showed that；realative BSAⅤSample protein structure(confomation)spectra, which is either protein main-chain FT-IR spectra lines(peaks),special for theamide plate-Ⅰ,1 652cm-1,or the protein side-chain amino acid residuce groups, Tyr, Phe, Trp, spectra lines 277.6 nm,216 nm(UV),340 nm,680 nm(FR),their peak intensity and the peak position all appeared great changers, and the changing degree of the radiating samples spectra is corresponding to the changing of the radiation parameters on the samples. It shows that laser is an important environmental factor to the cause the structure(comformation change)of protein in the microconic structure area, and make bio-signal transfer.