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Monitoring Dynamics of caspase-3 Activity in Living Cells Using FRET Technique during Apoptosis Induced by High Fluence LPLI
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WU Sheng-nan , XING Da
(MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science,
South China Normal University, Guangzhou 510631, Guangdong ,China )
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Abstract: Fluorescence resonance energy transfer ( FRET) can be used for measuring the distance between the molecules in living cells . In our study, We use FRET technique to measure the dynamics of caspase-3 activity in living cells during apoptosis induced by high fluence low-power laser irradiation (LPLI). The result showed that high fluence LPLI could induce apoptosis of ASTC-a-1 cells and FRET technique was an effective method to monitor dynamics of caspase-3 activity in apoptosis.
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